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Folding DNA Origami from a Double-Stranded Source of Scaffold

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, and Department of Cancer Biology, Dana Farber Cancer Institute, Boston, Massachusetts 02115
J. Am. Chem. Soc., 2009, 131 (26), pp 9154–9155
DOI: 10.1021/ja902569x
Publication Date (Web): June 16, 2009
Copyright © 2009 American Chemical Society
Cite this:J. Am. Chem. Soc.  131, 26, 9154-9155

Abstract

Abstract Image

Combined heat and chemical denaturation of double-stranded DNA scaffold strands in the presence of staple strands, followed by a sudden temperature drop and then stepwise dialysis to remove the chemical denaturant, leads to self-assembly of two distinct DNA-origami structures.

Additional TEM and gel images, computer code, sequences, and protocols. This material is available free via the Internet at http://pubs.acs.org.

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Article Views: 2,985 Times
Received 31 March 2009
Published online 16 June 2009
Published in print 8 July 2009
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