Programmable Transcriptional Modulation with a Structured RNA-Mediated CRISPR-dCas9 Complex
- Miao HeMiao HeSchool of Chemistry and Materials Science, Department of Polymer Science and Engineering, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), University of Science and Technology of China, Hefei, Anhui 230026, ChinaMore by Miao He
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- Xiang Zhou*Xiang Zhou*Email: [email protected]School of Chemistry and Materials Science, Department of Polymer Science and Engineering, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), University of Science and Technology of China, Hefei, Anhui 230026, ChinaMore by Xiang Zhou
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- Zhigang LiZhigang LiSchool of Chemistry and Materials Science, Department of Polymer Science and Engineering, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), University of Science and Technology of China, Hefei, Anhui 230026, ChinaMore by Zhigang Li
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- Xue YinXue YinSchool of Chemistry and Materials Science, Department of Polymer Science and Engineering, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), University of Science and Technology of China, Hefei, Anhui 230026, ChinaMore by Xue Yin
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- Wenjie HanWenjie HanSchool of Chemistry and Materials Science, Department of Polymer Science and Engineering, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), University of Science and Technology of China, Hefei, Anhui 230026, ChinaMore by Wenjie Han
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- Junxiang ZhouJunxiang ZhouSchool of Chemistry and Materials Science, Department of Polymer Science and Engineering, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), University of Science and Technology of China, Hefei, Anhui 230026, ChinaMore by Junxiang Zhou
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- Xiaoyun SunXiaoyun SunSchool of Chemistry and Materials Science, Department of Polymer Science and Engineering, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), University of Science and Technology of China, Hefei, Anhui 230026, ChinaMore by Xiaoyun Sun
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- Xiaoyu LiuXiaoyu LiuSchool of Chemistry and Materials Science, Department of Polymer Science and Engineering, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), University of Science and Technology of China, Hefei, Anhui 230026, ChinaMore by Xiaoyu Liu
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- Dongbao Yao*Dongbao Yao*Email: [email protected]School of Chemistry and Materials Science, Department of Polymer Science and Engineering, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), University of Science and Technology of China, Hefei, Anhui 230026, ChinaMore by Dongbao Yao
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- Haojun Liang*Haojun Liang*Email: [email protected]School of Chemistry and Materials Science, Department of Polymer Science and Engineering, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), University of Science and Technology of China, Hefei, Anhui 230026, ChinaMore by Haojun Liang
Abstract
Multi-module dCas9 engineering systems have been developed for controllable transcriptional manipulation such as chemical- or light-induced systems. However, there is still a need for a separate module that can be used for internal control over the CRISPR-dCas9 system. Here, we describe a multi-module CRISPR-dCas9 system in which a separate structured RNA was applied as a programmable component that could control dCas9-based gene regulation and achieved a higher activation efficiency than dCas9-VPR that is traditionally used. By introducing a microRNA sensor, we generated a dCas9-based transcriptional regulation platform that responded to endogenous microRNAs and allowed controllable activation of endogenous genes. Moreover, we applied the platform to selectively identify HCT116 cells in a cell mixture. This work provides a flexible platform for efficient and controllable gene regulation based on CRISPR-dCas9.
Cited By
This article is cited by 1 publications.
- Junxiang Zhou, Miao He, Xue Yin, Yue Yu, Dongbao Yao, Haojun Liang. Unexploited Performance of NLS in the dCas9-VPR-Mediated Transcriptional Activation. ACS Chemical Biology 2023, 18
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, 1246-1253. https://doi.org/10.1021/acschembio.3c00195