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Creation of a Synthetic Ligand for Mitochondrial DNA Sequence Recognition and Promoter-Specific Transcription Suppression

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    Creation of a Synthetic Ligand for Mitochondrial DNA Sequence Recognition and Promoter-Specific Transcription Suppression
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    † Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo, Kyoto 606-8502, Japan
    ‡ Institute for Integrated Cell-Material Science (WPI-iCeMS), Kyoto University, Sakyo, Kyoto 606-8501, Japan
    § Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, Imizu, Toyama 939-0398, Japan
    *[email protected]
    *[email protected]
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    Journal of the American Chemical Society

    Cite this: J. Am. Chem. Soc. 2017, 139, 25, 8444–8447
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    https://doi.org/10.1021/jacs.7b05230
    Published June 14, 2017
    Copyright © 2017 American Chemical Society
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    Abstract

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    Synthetic ligands capable of recognizing the specific DNA sequences inside human mitochondria and modulating gene transcription are in increasing demand because of the surge in evidence linking mitochondrial genome and diseases. In the work described herein, we created a new type of mitochondria-specific synthetic ligand, termed MITO-PIPs, by conjugating a mitochondria-penetrating peptide with pyrrole-imidazole polyamides (PIPs). The designed MITO-PIPs showed specific localization inside mitochondria in HeLa cells and recognized the target DNA in a sequence-specific manner. Furthermore, MITO-PIPs that inhibit the binding of mitochondrial transcription factor A to the light-strand promoter (LSP) also triggered targeted transcriptional suppression. The tunability of PIPs’ properties suggests the potential of the MITO-PIPs as potent modulators of not only mitochondrial gene transcription but also its DNA mutations.

    Copyright © 2017 American Chemical Society

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    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/jacs.7b05230.

    • Experimental procedures, characterization data for 1–4, primer list for qPCR experiments, and representative denaturation profiles in the Tm analyses, including Figures S1 and S2 and Table S1 (PDF)

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    38. Giacomo Padroni, John A Parkinson, Keith R Fox, Glenn A Burley. Structural basis of DNA duplex distortion induced by thiazole-containing hairpin polyamides. Nucleic Acids Research 2018, 46 (1) , 42-53. https://doi.org/10.1093/nar/gkx1211
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    Cite this: J. Am. Chem. Soc. 2017, 139, 25, 8444–8447
    Click to copy citationCitation copied!
    https://doi.org/10.1021/jacs.7b05230
    Published June 14, 2017
    Copyright © 2017 American Chemical Society
    Request reuse permissions

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