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Evaluation of the in Vivo Anti-inflammatory Effects of Extracts from Pyrus bretschneideri Rehd.

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School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China
School of Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
§ Institute of Chinese Matetria Medica, China Academy of Chinese Medicinal Sciences, Beijing 100700, China
Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China
*To whom correspondence should be addressed: School of Pharmaceutical Science and Technology, Tianjin University, Number 92, Weijin Road, Nankai District, Tianjin 300072, China. Telephone: 022-8740-1895. Fax: 022-8740-1895. E-mail: [email protected]
Cite this: J. Agric. Food Chem. 2010, 58, 16, 8983–8987
Publication Date (Web):July 30, 2010
https://doi.org/10.1021/jf101390q
Copyright © 2010 American Chemical Society

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    Abstract

    Pyrus bretschneideri Rehd., as a pharmaceutical supplement, is widely used in northern China to treat respiratory diseases. Our previous studies showed the ethanol extract of P. bretschneideri had significant anti-inflammatory activity. To isolate and identify the active ingredients, the ethanol extract was separated into petroleum ether, ethyl acetate, n-butanol, and aqueous fractions. The bioactivity of each fraction was investigated using an in vivo model. Results showed that the ethyl acetate fraction exhibited the strongest anti-inflammatory effect. Subsequently, this fraction was subjected to separation and purification using silica gel column chromatography, C18−ODS, and recrystallization, leading to two sterols and two triterpenes, which were identified as β-sitosterol, daucosterol, oleanolic acid, and ursolic acid. Moreover, all of the isolated compounds could significantly inhibit the ear edema induced by xylene. These results indicated that P. bretschneideri had good anti-inflammatory effects and the constituents β-sitosterol, daucosterol, oleanolic acid, and ursolic acid might well account for it.

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