Variation in Phenolic Content and Antioxidant Activity of Fermented Rooibos Herbal Tea Infusions: Role of Production Season and Quality Grade
- Elizabeth Joubert ,
- Theresa Beelders ,
- Dalene de Beer ,
- Christiaan J. Malherbe ,
- André J. de Villiers , and
- Gunnar O. Sigge
Abstract

Data are required to calculate the dietary exposure to rooibos herbal tea flavonoids and phenolic acids. Representative content values for the principal phenolic compounds and total antioxidant capacity of fermented rooibos infusion, taking into account variation caused by production seasons (2009, 2010, and 2011) and quality grades (A, B, C, and D), were determined for samples (n = 114) from different geographical areas and producers. The major phenolic constituents were isoorientin and orientin (>10 mg/L), with quercetin-3-O-robinobioside, phenylpyruvic acid glucoside, and aspalathin present at >5 mg/L. Isovitexin, vitexin, and hyperoside were present at <3 mg/L. Rutin, ferulic acid, and isoquercitrin were present at <2 mg/L. Nothofagin was present at <1 mg/L. Only traces of luteolin-7-O-glucoside and the aglycones quercetin, luteolin, and chrysoeriol were present. Substantial variation was observed in the individual content values of the phenolic compounds and total antioxidant capacity within production seasons and quality grades.
Cited By
This article is cited by 37 publications.
- Bernd Nidetzky, Alexander Gutmann, Chao Zhong. Leloir Glycosyltransferases as Biocatalysts for Chemical Production. ACS Catalysis 2018, 8 (7) , 6283-6300. https://doi.org/10.1021/acscatal.8b00710
- Khanyisani Ziqubu, Phiwayinkosi V. Dludla, Elizabeth Joubert, Christo J.F. Muller, Johan Louw, Luca Tiano, Bongani B. Nkambule, Abidemi P. Kappo, Sithandiwe E. Mazibuko-Mbeje. Isoorientin: A dietary flavone with the potential to ameliorate diverse metabolic complications. Pharmacological Research 2020, 158 , 104867. https://doi.org/10.1016/j.phrs.2020.104867
- Amsha Viraragavan, Nokulunga Hlengwa, Dalene de Beer, Sylvia Riedel, Neil Miller, Sandra Bowles, Beata Walczak, Christo Muller, Elizabeth Joubert. Model development for predicting in vitro bio-capacity of green rooibos extract based on composition for application as screening tool in quality control. Food & Function 2020, 11 (4) , 3084-3094. https://doi.org/10.1039/C9FO02480H
- Zuzanna Malyjurek, Dalene de Beer, Elizabeth Joubert, Beata Walczak. Working with log-ratios. Analytica Chimica Acta 2019, 1059 , 16-27. https://doi.org/10.1016/j.aca.2019.01.041
- Peng Zhou, Ou Hu, Haiyan Fu, Liqun Ouyang, Xuedong Gong, Peng Meng, Zheng Wang, Ming Dai, Xiaoming Guo, Ying Wang. UPLC–Q-TOF/MS-based untargeted metabolomics coupled with chemometrics approach for Tieguanyin tea with seasonal and year variations. Food Chemistry 2019, 283 , 73-82. https://doi.org/10.1016/j.foodchem.2019.01.050
- Manzimase Sishi, Magdalena Muller, Dalene de Beer, Marieta van der Rijst, Elizabeth Joubert. Rooibos agro‐processing waste as herbal tea products: optimisation of soluble solids extraction from dust and application to improve sensory profile, colour and flavonoid content of stem infusions. Journal of the Science of Food and Agriculture 2019, 99 (7) , 3653-3661. https://doi.org/10.1002/jsfa.9587
- Sithandiwe Mazibuko-Mbeje, Phiwayinkosi Dludla, Candice Roux, Rabia Johnson, Samira Ghoor, Elizabeth Joubert, Johan Louw, Andy Opoku, Christo Muller. Aspalathin-Enriched Green Rooibos Extract Reduces Hepatic Insulin Resistance by Modulating PI3K/AKT and AMPK Pathways. International Journal of Molecular Sciences 2019, 20 (3) , 633. https://doi.org/10.3390/ijms20030633
- Solomon Habtemariam. The chemical and pharmacological basis of rooibos (Aspalathus linearis (Burm. F.) R. Dahlgren) as potential therapy for type 2 diabetes and metabolic syndrome. 2019,,, 907-941. https://doi.org/10.1016/B978-0-08-102922-0.00023-7
- Carine Smith, Amanda Swart. Aspalathus linearis (Rooibos) – a functional food targeting cardiovascular disease. Food & Function 2018, 9 (10) , 5041-5058. https://doi.org/10.1039/C8FO01010B
- Katharina Schmölzer, Martin Lemmerer, Bernd Nidetzky. Glycosyltransferase cascades made fit for chemical production: Integrated biocatalytic process for the natural polyphenol C -glucoside nothofagin. Biotechnology and Bioengineering 2018, 115 (3) , 545-556. https://doi.org/10.1002/bit.26491
- Hong Xie, Xican Li, Zhenxing Ren, Weimin Qiu, Jianlan Chen, Qian Jiang, Ban Chen, Dongfeng Chen. Antioxidant and Cytoprotective Effects of Tibetan Tea and Its Phenolic Components. Molecules 2018, 23 (2) , 179. https://doi.org/10.3390/molecules23020179
- Christo J. F. Muller, Christiaan J. Malherbe, Nireshni Chellan, Kazumi Yagasaki, Yutaka Miura, Elizabeth Joubert. Potential of rooibos, its major C -glucosyl flavonoids, and Z -2-( β -D-glucopyranosyloxy)-3-phenylpropenoic acid in prevention of metabolic syndrome. Critical Reviews in Food Science and Nutrition 2018, 58 (2) , 227-246. https://doi.org/10.1080/10408398.2016.1157568
- Phiwayinkosi V. Dludla, Elizabeth Joubert, Christo J.F. Muller, Johan Louw, Rabia Johnson. Hyperglycemia-induced oxidative stress and heart disease-cardioprotective effects of rooibos flavonoids and phenylpyruvic acid-2-O-β-D-glucoside. Nutrition & Metabolism 2017, 14 (1) https://doi.org/10.1186/s12986-017-0200-8
- Élisabeth Millara. Survivre au régime alimentaire occidental. Pratiques en nutrition 2017, 13 (51) , 35-38. https://doi.org/10.1016/j.pranut.2017.06.009
- Leslie A. Nash, Wendy E. Ward. Tea and bone health: Findings from human studies, potential mechanisms, and identification of knowledge gaps. Critical Reviews in Food Science and Nutrition 2017, 57 (8) , 1603-1617. https://doi.org/10.1080/10408398.2014.1001019
- W.C.A. Gelderblom, E. Joubert, K. Gamieldien, L. Sissing, C.J. Malherbe, G. Maritz. Rooibos (Aspalathus linearis), honeybush (Cyclopia intermedia) and cancer bush (Sutherlandia frutescens subsp. microphylla) protect against tobacco-specific mutagenesis in vitro. South African Journal of Botany 2017, 110 , 194-200. https://doi.org/10.1016/j.sajb.2016.06.004
- Tao Zhang, Zhijie Fang. The concise synthesis and biological evaluation of C-glycosyl chalcone analogues inspired by the natural product aspalathin. RSC Advances 2017, 7 (5) , 3021-3024. https://doi.org/10.1039/C6RA26969A
- Carine Smith, Amanda C. Swart. Rooibos ( Aspalathus linearis ) facilitates an anti-inflammatory state, modulating IL-6 and IL-10 while not inhibiting the acute glucocorticoid response to a mild novel stressor in vivo. Journal of Functional Foods 2016, 27 , 42-54. https://doi.org/10.1016/j.jff.2016.08.055
- Gustavo Costa, Helena Grangeia, Artur Figueirinha, Isabel Vitória Figueiredo, Maria Teresa Batista. Influence of harvest date and material quality on polyphenolic content and antioxidant activity of Cymbopogon citratus infusion. Industrial Crops and Products 2016, 83 , 738-745. https://doi.org/10.1016/j.indcrop.2015.12.008
- E. Joubert, B. Jolley, I.S. Koch, M. Muller, M. Van der Rijst, D. de Beer. Major production areas of rooibos ( Aspalathus linearis ) deliver herbal tea of similar phenolic and phenylpropenoic acid glucoside content. South African Journal of Botany 2016, 103 , 162-169. https://doi.org/10.1016/j.sajb.2015.08.015
- André de Villiers, Pieter Venter, Harald Pasch. Recent advances and trends in the liquid-chromatography–mass spectrometry analysis of flavonoids. Journal of Chromatography A 2016, 1430 , 16-78. https://doi.org/10.1016/j.chroma.2015.11.077
- Alexandra E. Schulze, Theresa Beelders, Ilona S. Koch, Lené M. Erasmus, Dalene De Beer, Elizabeth Joubert. Honeybush herbal teas (Cyclopia spp.) contribute to high levels of dietary exposure to xanthones, benzophenones, dihydrochalcones and other bioactive phenolics. Journal of Food Composition and Analysis 2015, 44 , 139-148. https://doi.org/10.1016/j.jfca.2015.08.002
- Johanna van der Merwe, Dalene de Beer, Elizabeth Joubert, Wentzel Gelderblom. Short-Term and Sub-Chronic Dietary Exposure to Aspalathin-Enriched Green Rooibos (Aspalathus linearis) Extract Affects Rat Liver Function and Antioxidant Status. Molecules 2015, 20 (12) , 22674-22690. https://doi.org/10.3390/molecules201219868
- Federico Marini, Dalene de Beer, Elizabeth Joubert, Beata Walczak. Analysis of variance of designed chromatographic data sets: The analysis of variance-target projection approach. Journal of Chromatography A 2015, 1405 , 94-102. https://doi.org/10.1016/j.chroma.2015.05.060
- Leslie A. Nash, Philip J. Sullivan, Sandra J. Peters, Wendy E. Ward. Rooibos flavonoids, orientin and luteolin, stimulate mineralization in human osteoblasts through the Wnt pathway. Molecular Nutrition & Food Research 2015, 59 (3) , 443-453. https://doi.org/10.1002/mnfr.201400592
- Dalene de Beer, Christiaan J. Malherbe, Theresa Beelders, Elize L. Willenburg, D. Jacobus Brand, Elizabeth Joubert. Isolation of aspalathin and nothofagin from rooibos (Aspalathus linearis) using high-performance countercurrent chromatography: Sample loading and compound stability considerations. Journal of Chromatography A 2015, 1381 , 29-36. https://doi.org/10.1016/j.chroma.2014.12.078
- Nana Mikami, Junko Tsujimura, Ayumi Sato, Akiko Narasada, Mayumi Shigeta, Motoshi Kato, Shuichi Hata, Eri Hitomi. Green Rooibos Extract from Aspalathus linearis, and its Component, Aspalathin, Suppress Elevation of Blood Glucose Levels in Mice and Inhibit α-amylase and α-glucosidase Activities in vitro. Food Science and Technology Research 2015, 21 (2) , 231-240. https://doi.org/10.3136/fstr.21.231
- Joanna Orzel, Michal Daszykowski. A rapid validation of the antioxidant capacity of food commodities based on their fluorescence excitation emission spectra as applicable to coffee and peppermint extracts. Chemometrics and Intelligent Laboratory Systems 2014, 137 , 74-81. https://doi.org/10.1016/j.chemolab.2014.06.014
- Joanna Orzel, Michal Daszykowski, Malgorzata Kazura, Dalene de Beer, Elizabeth Joubert, Alexandra E. Schulze, Theresa Beelders, André de Villiers, Christiaan J. Malherbe, Beata Walczak. Modeling of the total antioxidant capacity of rooibos (Aspalathus linearis) tea infusions from chromatographic fingerprints and identification of potential antioxidant markers. Journal of Chromatography A 2014, 1366 , 101-109. https://doi.org/10.1016/j.chroma.2014.09.030
- Sha Li, Shu-Ke Li, Hua-Bin Li, Xiang-Rong Xu, Gui-Fang Deng, Dong-Ping Xu. Antioxidant Capacities of Herbal Infusions. 2014,,, 41-50. https://doi.org/10.1016/B978-0-12-404738-9.00005-2
- Micheline Sanderson, Sithandiwe E. Mazibuko, Elizabeth Joubert, Dalene de Beer, Rabia Johnson, Carmen Pheiffer, Johan Louw, Christo J.F. Muller. Effects of fermented rooibos (Aspalathus linearis) on adipocyte differentiation. Phytomedicine 2014, 21 (2) , 109-117. https://doi.org/10.1016/j.phymed.2013.08.011
- Christo J. F. Muller, Elizabeth Joubert, Carmen Pheiffer, Samira Ghoor, Micheline Sanderson, Nireshni Chellan, Stephen J. Fey, Johan Louw. Z -2-(β -d -glucopyranosyloxy)-3-phenylpropenoic acid, an α-hydroxy acid from rooibos ( Aspalathus linearis ) with hypoglycemic activity. Molecular Nutrition & Food Research 2013, 57 (12) , 2216-2222. https://doi.org/10.1002/mnfr.201300294
- Makoto Kondo, Yoshiaki Hirano, Masahiro Nishio, Yutaka Furuya, Hiromichi Nakamura, Tsuyoshi Watanabe. Xanthine Oxidase Inhibitory Activity and Hypouricemic Effect of Aspalathin from Unfermented Rooibos. Journal of Food Science 2013, 78 (12) , H1935-H1939. https://doi.org/10.1111/1750-3841.12304
- I. Stanimirova, M. Kazura, D. de Beer, E. Joubert, A.E. Schulze, T. Beelders, A. de Villiers, B. Walczak. High-dimensional nested analysis of variance to assess the effect of production season, quality grade and steam pasteurization on the phenolic composition of fermented rooibos herbal tea. Talanta 2013, 115 , 590-599. https://doi.org/10.1016/j.talanta.2013.06.023
- Madeline Simpson, Daisy Hjelmqvist, Camilo López-Alarcón, Nadja Karamehmedovic, Thomas Minehan, Akop Yepremyan, Baback Salehani, Eduardo Lissi, Elizabeth Joubert, Klas Udekwu, Emilio Alarcon. Anti-Peroxyl Radical Quality and Antibacterial Properties of Rooibos Infusions and Their Pure Glycosylated Polyphenolic Constituents. Molecules 2013, 18 (9) , 11264-11280. https://doi.org/10.3390/molecules180911264
- Piotr Zerzucha, Małgorzata Kazura, Dalene de Beer, Elizabeth Joubert, Alexandra E. Schulze, Theresa Beelders, André de Villiers, Beata Walczak. A new concept for variance analysis of hyphenated chromatographic data avoiding signal warping. Journal of Chromatography A 2013, 1291 , 64-72. https://doi.org/10.1016/j.chroma.2013.03.059
- Dalene de Beer, Alexandra Schulze, Elizabeth Joubert, André de Villiers, Christiaan Malherbe, Maria Stander. Food Ingredient Extracts of Cyclopia subternata (Honeybush): Variation in Phenolic Composition and Antioxidant Capacity. Molecules 2012, 17 (12) , 14602-14624. https://doi.org/10.3390/molecules171214602



