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    Triterpene Saponin Content in the Roots of Red Beet (Beta vulgaris L.) Cultivars
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    † Department of Plant Biochemistry, Faculty of Biology, University of Warsaw, Miecznikowa Street 1, 02-960 Warsaw, Poland
    ‡ Department of Biochemistry, Institute of Soil Science and Plant Cultivation, State Research Institute, Czartoryskich Street 8, 24-100 Puławy, Poland
    *Tel: +48 22554 3318. Fax: +48 22554 3221. E-mail: [email protected]
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    Journal of Agricultural and Food Chemistry

    Cite this: J. Agric. Food Chem. 2012, 60, 50, 12397–12402
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    https://doi.org/10.1021/jf303952x
    Published December 1, 2012
    Copyright © 2012 American Chemical Society
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    Triterpene saponins in the roots of Beta vulgaris cultivars Red Sphere, Rocket, and Wodan were profiled and quantitated using reverse-phase liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-ESI/MS/MS). Results obtained indicated that the roots of all three cultivars contained 11 saponins consisting of oleanolic acid or hederagenin aglycone and varying numbers of sugars, with the dominant triglycoside derivative of oleanolic acid. The relative proportions of derivatives of these two aglycones were similar in the three subspecies: cv. Red Sphere contained 99.1 and 0.9%; cv. Rocket, 98.2 and 1.8%; and Wodan 98.8 and 1.2% of oleanolic acid and hederagenin glycosides, respectively. To the best of our knowledge this is the first report on the occurrence, structure, and content of triterpenoid saponins in red beet.

    Copyright © 2012 American Chemical Society

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    9. Azza Hashim Abbas, Ramzi A. Abd Alsaheb, Jaafar Kamil Abdullah. Comparative study of natural chemical for enhanced oil recovery: Focus on extraction and adsorption at quartz sand surface. Petroleum 2023, 9 (1) , 83-93. https://doi.org/10.1016/j.petlm.2022.01.007
    10. Dóra Székely, Mónika Máté. Red Beetroot ( Beta Vulgaris L.). 2023https://doi.org/10.5772/intechopen.106692
    11. Pritam Kalia, Raman Selvakumar. Root Vegetables for Nutrition and Entrepreneurship. 2023, 481-532. https://doi.org/10.1007/978-981-19-9016-8_22
    12. Milad Norouzpour, Amin Azdarpour, Moein Nabipour, Rafael M. Santos, Abbas Khaksar Manshad, Stefan Iglauer, Hamed Akhondzadeh, Alireza Keshavarz. Red beet plant as a novel source of natural surfactant combined with ‘Smart Water’ for EOR purposes in carbonate reservoirs. Journal of Molecular Liquids 2023, 370 , 121051. https://doi.org/10.1016/j.molliq.2022.121051
    13. Julian Szymański, Dominik Szwajgier, Ewa Baranowska-Wójcik. The Role of Beetroot Ingredients in the Prevention of Alzheimer’s Disease. Applied Sciences 2023, 13 (2) , 1044. https://doi.org/10.3390/app13021044
    14. Ali Hosseini Motlagh, Ali Nasirpour, Sima Saeidy, Javad Keramat. Physicochemical and sensory properties of malt beverage containing sugar beet saponins. Journal of Food Science and Technology 2022, 59 (11) , 4380-4389. https://doi.org/10.1007/s13197-022-05517-x
    15. Sneh Punia Bangar, Nitya Sharma, Nikita Sanwal, Jose M. Lorenzo, J.K. Sahu. Bioactive potential of beetroot (Beta vulgaris). Food Research International 2022, 158 , 111556. https://doi.org/10.1016/j.foodres.2022.111556
    16. Aneta Spórna-Kucab, Anna Tekieli, Krystyna Skalicka-Woźniak, Agnieszka Grzegorczyk, Tomasz Świergosz, Sławomir Wybraniec. Characterization of Triterpene Saponin Composition of White, Yellow and Red Beetroot (Beta vulgaris L.). Polish Journal of Food and Nutrition Sciences 2022, , 159-170. https://doi.org/10.31883/pjfns/149515
    17. Wafa Grati, Sonda Samet, Bouthaina Bouzayani, Amani Ayachi, Michel Treilhou, Nathan Téné, Raoudha Mezghani-Jarraya. HESI-MS/MS Analysis of Phenolic Compounds from Calendula aegyptiaca Fruits Extracts and Evaluation of Their Antioxidant Activities. Molecules 2022, 27 (7) , 2314. https://doi.org/10.3390/molecules27072314
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    28. Aneta Spórna-Kucab, Sławomir Wybraniec. High-Speed Counter-Current Chromatography in Separation and Identification of Saponins from Beta vulgaris L. Cultivar Red Sphere. Polish Journal of Food and Nutrition Sciences 2020, 70 (1) , 67-74. https://doi.org/10.31883/pjfns/116416
    29. Marta Oleszek, Wieslaw Oleszek. Saponins in Food. 2020, 1-40. https://doi.org/10.1007/978-981-13-1745-3_34-1
    30. Deepu Pandita, Anu Pandita, Ramachandra Reddy Pamuru, Gulzar Ahmad Nayik. Beetroot. 2020, 45-74. https://doi.org/10.1007/978-981-15-7470-2_3
    31. Agnieszka Mroczek, Ireneusz Kapusta, Anna Stochmal, Wirginia Janiszowska. MS/MS and UPLC-MS profiling of triterpenoid saponins from leaves and roots of four red beet (Beta vulgaris L.) cultivars. Phytochemistry Letters 2019, 30 , 333-337. https://doi.org/10.1016/j.phytol.2019.02.015
    32. Theo Ralla, Hanna Salminen, Timo Wolfangel, Matthias Edelmann, Corinna Dawid, Thomas Hofmann, Jochen Weiss. Value addition of red beet ( Beta vulgaris L.) by‐products: Emulsion formation and stability. International Journal of Food Science & Technology 2019, 54 (3) , 619-625. https://doi.org/10.1111/ijfs.13886
    33. Noha Said Hussein, Azza S. Helmy, Naglaa M. Sherif, Hassan Z. Ghanem, Nabaweya A. Ibrahim, Abdel Nasser G. El Gendy, Abdel-Hamid Z. Abdel-Hamid. Lipidomic analysis reveals the efficiency of Eclipta prostrata on diet-induced nonalcoholic fatty liver disease in rats. Journal of Pharmaceutical and Biomedical Analysis 2019, 165 , 224-232. https://doi.org/10.1016/j.jpba.2018.11.060
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    35. Navnidhi Chhikara, Komal Kushwaha, Paras Sharma, Yogesh Gat, Anil Panghal. Bioactive compounds of beetroot and utilization in food processing industry: A critical review. Food Chemistry 2019, 272 , 192-200. https://doi.org/10.1016/j.foodchem.2018.08.022
    36. Bingjun Qian, Lirong Yin, Xiaomin Yao, Yaoguang Zhong, Juan Gui, Feifeng Lu, Fuming Zhang, Jianhua Zhang. Effects of fermentation on the hemolytic activity and degradation of Camellia oleifera saponins by Lactobacillus crustorum and Bacillus subtilis. FEMS Microbiology Letters 2018, 365 (7) https://doi.org/10.1093/femsle/fny014
    37. Raquel Mateos, Gema Baeza, Sara Martínez-López, Beatriz Sarriá, Laura Bravo. LC–MS n characterization of saponins in mate ( Ilex paraguariens, St. Hil) and their quantification by HPLC-DAD. Journal of Food Composition and Analysis 2017, 63 , 164-170. https://doi.org/10.1016/j.jfca.2017.08.003
    38. Diego dos S. Baião, Davi V.T. da Silva, Eduardo M. Del Aguila, Vânia M. Flosi Paschoalin. Nutritional, Bioactive and Physicochemical Characteristics of Different Beetroot Formulations. 2017https://doi.org/10.5772/intechopen.69301
    39. Katarzyna Mikołajczyk-Bator, Alfred Błaszczyk, Mariusz Czyżniejewski, Piotr Kachlicki. Identification of saponins from sugar beet ( Beta vulgaris ) by low and high-resolution HPLC–MS/MS. Journal of Chromatography B 2016, 1029-1030 , 36-47. https://doi.org/10.1016/j.jchromb.2016.06.038
    40. Katarzyna Mikołajczyk-Bator, Alfred Błaszczyk, Mariusz Czyżniejewski, Piotr Kachlicki. Characterisation and identification of triterpene saponins in the roots of red beets (Beta vulgaris L.) using two HPLC–MS systems. Food Chemistry 2016, 192 , 979-990. https://doi.org/10.1016/j.foodchem.2015.07.111
    41. T. K. Lim. Beta vulgaris. 2016, 26-68. https://doi.org/10.1007/978-94-017-7276-1_2
    42. Fusun Oztay, Ozlem Sacan, Ozgecan Kayalar, Sehnaz Bolkent, Yesim Ipci, Levent Kabasakal, Goksel Sener, Refiye Yanardag. Chard ( Beta vulgaris var. cicla ) extract improved hyperglycemia-induced oxidative stress and surfactant-associated protein alterations in rat lungs. Pharmaceutical Biology 2015, 53 (11) , 1639-1646. https://doi.org/10.3109/13880209.2014.997252
    43. Agnieszka Mroczek. Phytochemistry and bioactivity of triterpene saponins from Amaranthaceae family. Phytochemistry Reviews 2015, 14 (4) , 577-605. https://doi.org/10.1007/s11101-015-9394-4
    44. Yun Zhao, Lu Peng, Wei Lu, Yiqing Wang, Xuefeng Huang, Chen Gong, Lin He, Junhao Hong, Songsong Wu, Xin Jin. Effect of Eclipta prostrata on lipid metabolism in hyperlipidemic animals. Experimental Gerontology 2015, 62 , 37-44. https://doi.org/10.1016/j.exger.2014.12.017
    45. Beaudelaire K. Ponou, Raymond N. Nono, Rémy B. Teponno, Azefack L. Tapondjou, Marie-Aleth Lacaille-Dubois, Luana Quassinti, Massimo Bramucci, Luciano Barboni. Bafouoside C, a new triterpenoid saponin from the roots of Cussonia bancoensis Aubrev. & Pellegr.. Phytochemistry Letters 2014, 10 , 255-259. https://doi.org/10.1016/j.phytol.2014.10.007
    46. Irma Podolak, Paweł Żmudzki, Paulina Koczurkiewicz, Marta Michalik, Paweł Zajdel, Agnieszka Galanty. Minor Triterpene Saponins from Underground Parts of Lysimachia thyrsiflora : Structure elucidation, LC-ESI-MS/MS Quantification, and Biological Activity. Natural Product Communications 2013, 8 (12) https://doi.org/10.1177/1934578X1300801208
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    Cite this: J. Agric. Food Chem. 2012, 60, 50, 12397–12402
    Click to copy citationCitation copied!
    https://doi.org/10.1021/jf303952x
    Published December 1, 2012
    Copyright © 2012 American Chemical Society
    Request reuse permissions

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