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Inhibitory Effects of Wild Blueberry Anthocyanins and Other Flavonoids on Biomarkers of Acute and Chronic Inflammation in Vitro

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Plants for Human Health Institute, North Carolina State University, 600 Laureate Way, Kannapolis, North Carolina 28081, United States
Department of Food, Bioprocessing, and Nutrition Sciences, North Carolina State University, 400 Dan Allen Drive, Raleigh, North Carolina 27695, United States
§ Department of Biology, Duke University, 125 Science Drive, Durham, North Carolina 27708, United States
*(M.A.L.) Phone: (704) 250-5407. Fax: (704) 250-5409. E-mail: [email protected]
Cite this: J. Agric. Food Chem. 2014, 62, 29, 7022–7028
Publication Date (Web):January 8, 2014
https://doi.org/10.1021/jf4051599
Copyright © 2014 American Chemical Society
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Abstract

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Wild lowbush blueberries (Vaccinium angustifolium Ait) are a rich source of anthocyanins and other flavonoids with anti-inflammatory activities; however, their individual effects on cellular signaling remain to be elucidated. This study determined the capacity of blueberry bioactives to protect murine RAW 264.7 macrophages from lipopolysaccharide-induced inflammation. Fractionation of the crude extract (CE) into polyphenol-rich (PPR), anthocyanin-rich (ANC), and proanthocyanidin-rich (PAC) fractions and an ethyl acetate fraction (EA) revealed that PPR, ANC, and PAC components most effectively suppressed mRNA biomarkers of acute inflammation (Cox-2, iNOS, and IL-1β). Among major polyphenols found in the wild blueberries, malvidin-3-glucoside was significantly more effective than epicatechin or chlorogenic acid in reducing the expression of pro-inflammatory genes in vitro.

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