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Identification of Metabolites in Human Plasma and Urine after Consumption of a Polyphenol-Rich Juice Drink

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    Article

    Identification of Metabolites in Human Plasma and Urine after Consumption of a Polyphenol-Rich Juice Drink
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    † Division of Ecology and Evolutionary Biology, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, United Kingdom
    ‡ Division of Developmental Medicine, Faculty of Medicine, University of Glasgow, Glasgow G12 8QQ, United Kingdom
    § Unit of Human Nutrition, Division of Developmental Medicine, Queen Elizabeth Building, Royal Infirmary, University of Glasgow, Glasgow G31 2ER, United Kingdom
    # Global Scientific and Regulatory Affairs, The Coca-Cola Company, Atlanta, Georgia 30313
    *Author to whom correspondence should be addressed (telephone +44-141-330-4613; fax +44-141-330-5394; e-mail [email protected]).
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    Journal of Agricultural and Food Chemistry

    Cite this: J. Agric. Food Chem. 2010, 58, 4, 2586–2595
    Click to copy citationCitation copied!
    https://doi.org/10.1021/jf904096v
    Published January 20, 2010
    Copyright © 2010 American Chemical Society
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    Abstract

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    A polyphenol-rich (P-R) juice drink was developed as a potential approach to increase intake of dietary polyphenols. Analysis of the beverage by HPLC with PDA, fluorescence, and MS detection facilitated the identification/partial identification of 40 flavonoids and related phenolic compounds. The main constituents were (−)-epigallocatechin and other green tea flavan-3-ols, phloretin-2′-O-glucoside, gallic acid, hesperetin-7-O-rutinoside, 5-O-caffeoylquinic acid, and procyanidins, with trace levels of several flavonols and purple grape juice anthocyanins also being present. Healthy human subjects (n = 10) consumed 350 mL of the P-R juice drink, after which plasma and urine samples were collected over a 0−24 h period. HPLC-MS analysis identified 13 metabolites in plasma and a further 20 in urine. Qualitatively, the profiles of the glucuronide, sulfated, and methylated metabolites were very similar to those detected in earlier investigations when the main components in the juice drink were consumed separately in feeding studies with coffee, green tea, orange juice, and apple cider.

    Copyright © 2010 American Chemical Society

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    This article is cited by 42 publications.

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    Journal of Agricultural and Food Chemistry

    Cite this: J. Agric. Food Chem. 2010, 58, 4, 2586–2595
    Click to copy citationCitation copied!
    https://doi.org/10.1021/jf904096v
    Published January 20, 2010
    Copyright © 2010 American Chemical Society
    Request reuse permissions

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