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Decorated Rods:  A “Bottom-Up” Self-Assembly of Monomolecular DNA Complexes

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    Decorated Rods:  A “Bottom-Up” Self-Assembly of Monomolecular DNA Complexes
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    Departments of Physics and Pharmaceutical Biology-Biotechnology, Ludwig-Maximilians-Universität, D-80539 Munich, Germany
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    The Journal of Physical Chemistry B

    Cite this: J. Phys. Chem. B 2006, 110, 10, 4548–4554
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    https://doi.org/10.1021/jp053760a
    Published February 18, 2006
    Copyright © 2006 American Chemical Society
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    Abstract

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    Fluorescence correlation spectroscopy (FCS) and gel electrophoresis measurements are performed to investigate both the number and size of complexes of linear double-stranded DNA (dsDNA) fragments with 1:1 diblock copolymers consisting of a cationic moiety, branched polyethyleneimine (bPEI) of 2, 10, or 25 kDa, covalently bound to a neutral shielding moiety, poly(ethylene glycol) (PEG; 20 kDa). By systematically decreasing the bPEI length, the PEG grafting density along the DNA chain can be directly controlled. For 25 and 10 kDa bPEI−PEG copolymers, severe aggregation is observed despite the presence of the shielding PEG. Upon decreasing the bPEI length to 2 kDa, controlled self-assembly of monomolecular DNA nanoparticles is observed. The resulting complexes are in quantitative agreement with a theoretical model based on a single DNA encased in a dense PEG polymer brush layer. The resulting PEGylated complexes show high stability against both salt and protein and hence are of potential use for in vivo gene delivery studies.

    Copyright © 2006 American Chemical Society

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     To whom correspondence should be addressed. Current address:  Laboratory of Physical and Structural Biology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892-0924. E-mail:  [email protected].

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    Cite this: J. Phys. Chem. B 2006, 110, 10, 4548–4554
    Click to copy citationCitation copied!
    https://doi.org/10.1021/jp053760a
    Published February 18, 2006
    Copyright © 2006 American Chemical Society
    Request reuse permissions

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