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Enhanced Catalytic Activity of Lipase Encapsulated in PCL Nanofibers

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Interdisciplinary Nanoscience Center (iNANO) and §Department of Engineering, Aarhus University, Aarhus C DK-8000, Denmark
School of Physical Science and Technology, Lanzhou University, Lanzhou 730000, People’s Republic of China
Cite this: Langmuir 2012, 28, 14, 6157–6162
Publication Date (Web):March 7, 2012
https://doi.org/10.1021/la300469s
Copyright © 2012 American Chemical Society
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Abstract

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Use of biocatalysis for industrial synthetic chemistry is on the verge of significant growth. Enzyme immobilization as an effective strategy for improving the enzyme activity has emerged from developments especially in nanoscience and nanotechnology. Here, lipase from Burkholderia cepacia (LBC), as an example of the luxuriant enzymes, was successfully encapsulated in polycaprolactone (PCL) nanofibers, proven by X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). Evaluated in both organic and aqueous medium, the activation factor of the encapsulated enzymes in the hydrolysis reaction was generally higher than that in the transesterification reaction. Enhanced catalytic activities were found when 5–20 w/w % of LBC was loaded. The effect of different solvents pretreatment on the activity of immobilized LBC was also investigated. The highest activation factor was found up to 14 for the sample containing acetone-treated LBC/PCL (10 w/w %). The encapsulated lipase reserved 50% of its original activity after the 10th run in the transesterification reaction in hexane medium. The mechanism of activation of lipase catalytic ability based on active PCL nanofiberous matrix is proposed.

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  7. Jing An, Galong Li, Yifan Zhang, Tingbin Zhang, Xiaoli Liu, Fei Gao, Mingli Peng, Yuan He, Haiming Fan. Recent Advances in Enzyme-Nanostructure Biocatalysts with Enhanced Activity. Catalysts 2020, 10 (3) , 338. https://doi.org/10.3390/catal10030338
  8. Ceyhun Işik, Gökmen Arabaci, Yasemin Ispirli Doğaç, İlyas Deveci, Mustafa Teke. Synthesis and characterization of electrospun PVA/Zn2+ metal composite nanofibers for lipase immobilization with effective thermal, pH stabilities and reusability. Materials Science and Engineering: C 2019, 99 , 1226-1235. https://doi.org/10.1016/j.msec.2019.02.031
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  22. Matthew S. Muroski, Om V. Singh. Implications of Nanotechnology into Next Generation Biofuel Industry. 2015,,, 452-476. https://doi.org/10.4018/978-1-4666-6363-3.ch021
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