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Formation of Supported Lipid Bilayers by Vesicle Fusion: Effect of Deposition Temperature

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Nano-Science Center and Institute of Chemistry, Copenhagen University, Copenhagen, Denmark
European Spallation Source ESS AB, Lund, Sweden
§ Malmoe University, Health & Society, 20506 Malmoe, Sweden
Cite this: Langmuir 2014, 30, 25, 7259–7263
Publication Date (Web):June 16, 2014
https://doi.org/10.1021/la500897x
Copyright © 2014 American Chemical Society
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Abstract

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We have investigated the effect of deposition temperature on supported lipid bilayer formation via vesicle fusion. By using several complementary surface-sensitive techniques, we demonstrate that despite contradicting literature on the subject, high-quality bilayers can be formed below the main phase-transition temperature of the lipid. We have carefully studied the formation mechanism of supported DPPC bilayers below and above the lipid melting temperature (Tm) by quartz crystal microbalance and atomic force microscopy under continuous flow conditions. We also measured the structure of lipid bilayers formed below or above Tm by neutron reflection and investigated the effect of subsequent cooling to below the Tm. Our results clearly show that a continuous supported bilayer can be formed with high surface coverage below the lipid Tm. We also demonstrate that the high dissipation responses observed during the deposition process by QCM-D correspond to vesicles absorbed on top of a continuous bilayer and not to a surface-supported vesicular layer as previously reported.

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QCM-D traces for the deposition of DPPC bilayers in buffer and in pure water at 25 and at 50 °C. Additional information on neutron reflection measurements, fitting procedures, and models. SAXS data of multilamellar DPPC vesicles used to obtain the lamellar repeat distance. This material is available free of charge via the Internet at http://pubs.acs.org.

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