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    Yielding Behavior in Injectable Hydrogels from Telechelic Proteins
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    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, United States
    *To whom correspondence should be addressed. E-mail: [email protected] (D.A.T.) or [email protected] (J.A.K.).
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    Macromolecules

    Cite this: Macromolecules 2010, 43, 21, 9094–9099
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    https://doi.org/10.1021/ma101434a
    Published October 13, 2010
    Copyright © 2010 American Chemical Society
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    Abstract

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    Injectable hydrogels show substantial promise for use in minimally invasive tissue engineering and drug delivery procedures. A new injectable hydrogel material, developed from recombinant telechelic proteins expressed in E. coli, demonstrates shear thinning by 3 orders of magnitude at large strains. Large-amplitude oscillatory shear illustrates that shear thinning is due to yielding within the bulk of the gel, and the rheological response and flow profiles are consistent with a shear-banding mechanism for yielding. The sharp yielding transition and large magnitude of the apparent shear thinning allow gels to be injected through narrow gauge needles with only gentle hand pressure. After injection the gels reset to full elastic strength in seconds due to rapid re-formation of the physical network junctions, allowing self-supporting structures to be formed. The shear thinning and recovery behavior is largely independent of the midblock length, enabling genetic engineering to be used to control the equilibrium modulus of the gel without loss of the characteristic yielding behavior. The shear-banding mechanism localizes deformation during flow into narrow regions of the gels, allowing more than 95% of seeded cells to survive the injection process.

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    Macromolecules

    Cite this: Macromolecules 2010, 43, 21, 9094–9099
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    https://doi.org/10.1021/ma101434a
    Published October 13, 2010
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