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Synthetic Protein Targeting by the Intrinsic Biorecognition Functionality of Poly(ethylene glycol) Using PEG Antibodies as Biohybrid Molecular Adaptors

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Biozentrum and the Swiss Nanoscience Institute, University of Basel, Klingelbergstrasse 70, Basel 4056, Switzerland
Institute of Materials Research and Engineering, A*STAR (Agency for Science, Technology and Research), 3 Research Link, Singapore 117602
Address correspondence to [email protected]
Cite this: ACS Nano 2011, 5, 6, 5180–5187
Publication Date (Web):May 31, 2011
https://doi.org/10.1021/nn201327y
Copyright © 2011 American Chemical Society
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Abstract

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Biointerfaces capable of biological recognition and specificity are sought after for conferring bioinspired functionality onto synthetic biomaterials systems. This is important for biosensing, bioseparations, and biomedical materials. Here, we demonstrate how intrinsic polymer–protein interactions between highly localized polyethylene glycol (PEG) brushes and PEG-binding antibodies can be used for sorting specific biomolecules from complex bulk biological fluids to synthetic nanoscale targets. A principal feature lies with the antifouling property of PEG that prevents unspecific binding. Exclusive access is provided by anti-PEG, which acts as a biohybrid molecular adaptor that sifts out and targets specific IgG “cargo” from solution to the PEG. The PEG can be reversibly washed and targeted in blood serum, which suggests potential benefits in technological applications. Moreover, anti-PEG binding triggers a stimuli-responsive conformational collapse in the PEG brush, thereby imparting an intrinsic “smart” biorecognition functionality to the PEG that can considerably impact its use as an antifouling biomaterial.

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Figure S1, immunoblot and binding affinity measurements by surface plasmon resonance; Figure S2, representative force curves; and Figure S3, sequential binding by time-lapse TIRF. This material is available free of charge via the Internet at http://pubs.acs.org.

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Cited By


This article is cited by 9 publications.

  1. Gustav Emilsson, Yusuke Sakiyama, Bita Malekian, Kunli Xiong, Zeynep Adali-Kaya, Roderick Y. H. Lim, Andreas B. Dahlin. Gating Protein Transport in Solid State Nanopores by Single Molecule Recognition. ACS Central Science 2018, 4 (8) , 1007-1014. https://doi.org/10.1021/acscentsci.8b00268
  2. Bing-Mae Chen, Yu-Cheng Su, Chia-Jung Chang, Pierre-Alain Burnouf, Kuo-Hsiang Chuang, Chien-Hsiun Chen, Tian-Lu Cheng, Yuan-Tsong Chen, Jer-Yuarn Wu, and Steve R. Roffler . Measurement of Pre-Existing IgG and IgM Antibodies against Polyethylene Glycol in Healthy Individuals. Analytical Chemistry 2016, 88 (21) , 10661-10666. https://doi.org/10.1021/acs.analchem.6b03109
  3. Rafael L. Schoch and Roderick Y. H. Lim . Non-Interacting Molecules as Innate Structural Probes in Surface Plasmon Resonance. Langmuir 2013, 29 (12) , 4068-4076. https://doi.org/10.1021/la3049289
  4. Tian-Lu Cheng, Kuo-Hsiang Chuang, Bing-Mae Chen, and Steve R. Roffler . Analytical Measurement of PEGylated Molecules. Bioconjugate Chemistry 2012, 23 (5) , 881-899. https://doi.org/10.1021/bc200478w
  5. Gustav Emilsson, Kunli Xiong, Yusuke Sakiyama, Bita Malekian, Viktor Ahlberg Gagnér, Rafael L. Schoch, Roderick Y. H. Lim, Andreas B. Dahlin. Polymer brushes in solid-state nanopores form an impenetrable entropic barrier for proteins. Nanoscale 2018, 10 (10) , 4663-4669. https://doi.org/10.1039/C7NR09432A
  6. Jin Zhang, Kelan Liu, Klaus Müllen, Meizhen Yin. Self-assemblies of amphiphilic homopolymers: synthesis, morphology studies and biomedical applications. Chemical Communications 2015, 51 (58) , 11541-11555. https://doi.org/10.1039/C5CC03016A
  7. Basit Yameen, Aleeza Farrukh. Polymer Brushes: Promises and Challenges. Chemistry - An Asian Journal 2013, 8 (8) , 1736-1753. https://doi.org/10.1002/asia.201300149
  8. Szczepan Zapotoczny. Surface-Grafted Polymer Brushes. 2013,,, 27-43. https://doi.org/10.1002/9783527649600.ch2
  9. Janne T. Hyotyla, Roderick Y. H. Lim. Atomic Force Microscopy (AFM). 2012,,https://doi.org/10.1002/9780470661345.smc043

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