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Virus-Inspired Membrane Encapsulation of DNA Nanostructures To Achieve In Vivo Stability

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†Wyss Institute for Biologically Inspired Engineering and ‡Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, United States,
§ Department of Cancer Biology, Dana Farber Cancer Institute, Boston, Massachusetts 02115, United States
*Address correspondence to [email protected]
Cite this: ACS Nano 2014, 8, 5, 5132–5140
Publication Date (Web):April 2, 2014
https://doi.org/10.1021/nn5011914
Copyright © 2014 American Chemical Society
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DNA nanotechnology enables engineering of molecular-scale devices with exquisite control over geometry and site-specific functionalization. This capability promises compelling advantages in advancing nanomedicine; nevertheless, instability in biological environments and innate immune activation remain as obstacles for in vivo application. Natural particle systems (i.e., viruses) have evolved mechanisms to maintain structural integrity and avoid immune recognition during infection, including encapsulation of their genome and protein capsid shell in a lipid envelope. Here we introduce virus-inspired enveloped DNA nanostructures as a design strategy for biomedical applications. Achieving a high yield of tightly wrapped unilamellar nanostructures, mimicking the morphology of enveloped virus particles, required precise control over the density of attached lipid conjugates and was achieved at 1 per ∼180 nm2. Envelopment of DNA nanostructures in PEGylated lipid bilayers conferred protection against nuclease digestion. Immune activation was decreased 2 orders of magnitude below controls, and pharmacokinetic bioavailability improved by a factor of 17. By establishing a design strategy suitable for biomedical applications, we have provided a platform for the engineering of sophisticated, translation-ready DNA nanodevices.

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Methods, additional data, and a nanostructure design schematic and oligonucleotide sequences are available as Supporting Information. This material is available free of charge via the Internet at http://pubs.acs.org.

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  1. Shai Zilberzwige-Tal, Dan Mark Alon, Danielle Gazit, Shahar Zachariah, Amit Hollander, Ehud Gazit, Johann Elbaz. Genetically Encoding Ultrastable Virus-like Particles Encapsulating Functional DNA Nanostructures in Living Bacteria. ACS Synthetic Biology 2021, 10 (8) , 1798-1807. https://doi.org/10.1021/acssynbio.0c00586
  2. Fan Xu, Baijun Dong, Xue Li, Fei Gao, Donglei Yang, Wei Xue, Pengfei Wang. Profiling and Regulating Proteins That Adsorb to DNA Materials in Human Serum. Analytical Chemistry 2021, 93 (24) , 8671-8679. https://doi.org/10.1021/acs.analchem.1c02075
  3. Leo Y. T. Chou. Design Verification as Foundation for Advancing DNA Nanotechnology Applications. ACS Nano 2021, 15 (6) , 9222-9228. https://doi.org/10.1021/acsnano.1c04304
  4. Aurélie Lacroix, Hanadi F. Sleiman. DNA Nanostructures: Current Challenges and Opportunities for Cellular Delivery. ACS Nano 2021, 15 (3) , 3631-3645. https://doi.org/10.1021/acsnano.0c06136
  5. Nishkantha Arulkumaran, Conor Lanphere, Charlotte Gaupp, Jonathan R. Burns, Mervyn Singer, Stefan Howorka. DNA Nanodevices with Selective Immune Cell Interaction and Function. ACS Nano 2021, 15 (3) , 4394-4404. https://doi.org/10.1021/acsnano.0c07915
  6. Sampa Sarkar, Nhiem Tran, Sarvesh Kumar Soni, Charlotte E. Conn, Calum J. Drummond. Size-Dependent Encapsulation and Release of dsDNA from Cationic Lyotropic Liquid Crystalline Cubic Phases. ACS Biomaterials Science & Engineering 2020, 6 (8) , 4401-4413. https://doi.org/10.1021/acsbiomaterials.0c00085
  7. Xiaoli Sun, Haipeng Liu. Nucleic Acid Nanostructure Assisted Immune Modulation. ACS Applied Bio Materials 2020, 3 (5) , 2765-2778. https://doi.org/10.1021/acsabm.9b01195
  8. Arun Richard Chandrasekaran, Javier Vilcapoma, Paromita Dey, Siu Wah Wong-Deyrup, Bijan K. Dey, Ken Halvorsen. Exceptional Nuclease Resistance of Paranemic Crossover (PX) DNA and Crossover-Dependent Biostability of DNA Motifs. Journal of the American Chemical Society 2020, 142 (14) , 6814-6821. https://doi.org/10.1021/jacs.0c02211
  9. Kai Xia, Jianlei Shen, Qian Li, Chunhai Fan, Hongzhou Gu. Near-Atomic Fabrication with Nucleic Acids. ACS Nano 2020, 14 (2) , 1319-1337. https://doi.org/10.1021/acsnano.9b09163
  10. Frances M. Anastassacos, Zhao Zhao, Yang Zeng, William M. Shih. Glutaraldehyde Cross-Linking of Oligolysines Coating DNA Origami Greatly Reduces Susceptibility to Nuclease Degradation. Journal of the American Chemical Society 2020, 142 (7) , 3311-3315. https://doi.org/10.1021/jacs.9b11698
  11. Mengying Deng, Min Li, Fan Li, Xiuhai Mao, Qian Li, Jianlei Shen, Chunhai Fan, Xiaolei Zuo. Programming Accessibility of DNA Monolayers for Degradation-Free Whole-Blood Biosensors. ACS Materials Letters 2019, 1 (6) , 671-676. https://doi.org/10.1021/acsmaterialslett.9b00404
  12. Job A. L. Roodhuizen, Philip J. T. M. Hendrikx, Peter A. J. Hilbers, Tom F. A. de Greef, Albert J. Markvoort. Counterion-Dependent Mechanisms of DNA Origami Nanostructure Stabilization Revealed by Atomistic Molecular Simulation. ACS Nano 2019, 13 (9) , 10798-10809. https://doi.org/10.1021/acsnano.9b05650
  13. Ting Zhang, Jia Nong, Nouf Alzahrani, Zhicheng Wang, Sung Won Oh, Tristan Meier, Dong Gyu Yang, Yonggang Ke, Yinghui Zhong, Jinglin Fu. Self-Assembly of DNA–Minocycline Complexes by Metal Ions with Controlled Drug Release. ACS Applied Materials & Interfaces 2019, 11 (33) , 29512-29521. https://doi.org/10.1021/acsami.9b08126
  14. William L. Whitehouse, James E. Noble, Maxim G. Ryadnov, Stefan Howorka. Cholesterol Anchors Enable Efficient Binding and Intracellular Uptake of DNA Nanostructures. Bioconjugate Chemistry 2019, 30 (7) , 1836-1844. https://doi.org/10.1021/acs.bioconjchem.9b00036
  15. Yi Li, Rebecca Schulman. DNA Nanostructures that Self-Heal in Serum. Nano Letters 2019, 19 (6) , 3751-3760. https://doi.org/10.1021/acs.nanolett.9b00888
  16. Qinqin Hu, Hua Li, Lihua Wang, Hongzhou Gu, Chunhai Fan. DNA Nanotechnology-Enabled Drug Delivery Systems. Chemical Reviews 2019, 119 (10) , 6459-6506. https://doi.org/10.1021/acs.chemrev.7b00663
  17. Mikael Madsen, Kurt V. Gothelf. Chemistries for DNA Nanotechnology. Chemical Reviews 2019, 119 (10) , 6384-6458. https://doi.org/10.1021/acs.chemrev.8b00570
  18. Haijing Shen, Yingqian Wang, Jie Wang, Zhihao Li, Quan Yuan. Emerging Biomimetic Applications of DNA Nanotechnology. ACS Applied Materials & Interfaces 2019, 11 (15) , 13859-13873. https://doi.org/10.1021/acsami.8b06175
  19. Stefano Palazzolo, Mohamad Hadla, Concetta Russo Spena, Samer Bayda, Vinit Kumar, Francesco Lo Re, Muhammad Adeel, Isabella Caligiuri, Flavio Romano, Giuseppe Corona, Vincenzo Canzonieri, Giuseppe Toffoli, Flavio Rizzolio. Proof-of-Concept Multistage Biomimetic Liposomal DNA Origami Nanosystem for the Remote Loading of Doxorubicin. ACS Medicinal Chemistry Letters 2019, 10 (4) , 517-521. https://doi.org/10.1021/acsmedchemlett.8b00557
  20. Hyungmin Jun, Tyson R. Shepherd, Kaiming Zhang, William P. Bricker, Shanshan Li, Wah Chiu, Mark Bathe. Automated Sequence Design of 3D Polyhedral Wireframe DNA Origami with Honeycomb Edges. ACS Nano 2019, 13 (2) , 2083-2093. https://doi.org/10.1021/acsnano.8b08671
  21. Pongphak Chidchob, Daniel Offenbartl-Stiegert, Dillon McCarthy, Xin Luo, Jianing Li, Stefan Howorka, Hanadi F. Sleiman. Spatial Presentation of Cholesterol Units on a DNA Cube as a Determinant of Membrane Protein-Mimicking Functions. Journal of the American Chemical Society 2019, 141 (2) , 1100-1108. https://doi.org/10.1021/jacs.8b11898
  22. Zhilei Ge, Hongzhou Gu, Qian Li, Chunhai Fan. Concept and Development of Framework Nucleic Acids. Journal of the American Chemical Society 2018, 140 (51) , 17808-17819. https://doi.org/10.1021/jacs.8b10529
  23. Anand Lopez, Juewen Liu. DNA Oligonucleotide-Functionalized Liposomes: Bioconjugate Chemistry, Biointerfaces, and Applications. Langmuir 2018, 34 (49) , 15000-15013. https://doi.org/10.1021/acs.langmuir.8b01368
  24. Boxuan Shen, Mauri A. Kostiainen, Veikko Linko. DNA Origami Nanophotonics and Plasmonics at Interfaces. Langmuir 2018, 34 (49) , 14911-14920. https://doi.org/10.1021/acs.langmuir.8b01843
  25. Shoya Takidani, Kanna Aoki, Minoru Fujii. One-Step Discrete Symmetric Arrangement of Magnetic Microspheres with Nanoscale Spacing Immobilized by Ultraviolet Irradiation toward Plasmonic Resonators. ACS Applied Nano Materials 2018, 1 (11) , 6055-6062. https://doi.org/10.1021/acsanm.8b01166
  26. Lin Zhong, Shuxian Cai, Yuqing Huang, Litian Yin, Yuling Yang, Chunhua Lu, Huanghao Yang. DNA Octahedron-Based Fluorescence Nanoprobe for Dual Tumor-Related mRNAs Detection and Imaging. Analytical Chemistry 2018, 90 (20) , 12059-12066. https://doi.org/10.1021/acs.analchem.8b02847
  27. Fabian Kollmann, Saminathan Ramakrishnan, Boxuan Shen, Guido Grundmeier, Mauri A. Kostiainen, Veikko Linko, Adrian Keller. Superstructure-Dependent Loading of DNA Origami Nanostructures with a Groove-Binding Drug. ACS Omega 2018, 3 (8) , 9441-9448. https://doi.org/10.1021/acsomega.8b00934
  28. Maartje M. C. Bastings, Frances M. Anastassacos, Nandhini Ponnuswamy, Franziska G. Leifer, Garry Cuneo, Chenxiang Lin, Donald E. Ingber, Ju Hee Ryu, William M. Shih. Modulation of the Cellular Uptake of DNA Origami through Control over Mass and Shape. Nano Letters 2018, 18 (6) , 3557-3564. https://doi.org/10.1021/acs.nanolett.8b00660
  29. Jonathan R. Burns, Baptiste Lamarre, Alice L. B. Pyne, James E. Noble, Maxim G. Ryadnov. DNA Origami Inside-Out Viruses. ACS Synthetic Biology 2018, 7 (3) , 767-773. https://doi.org/10.1021/acssynbio.7b00278
  30. Tian Tian, Jiang Li, Cao Xie, Yanhong Sun, Haozhi Lei, Xinyi Liu, Jiaoyun Xia, Jiye Shi, Lihua Wang, Weiyue Lu, and Chunhai Fan . Targeted Imaging of Brain Tumors with a Framework Nucleic Acid Probe. ACS Applied Materials & Interfaces 2018, 10 (4) , 3414-3420. https://doi.org/10.1021/acsami.7b17927
  31. Fan Hong, Fei Zhang, Yan Liu, and Hao Yan . DNA Origami: Scaffolds for Creating Higher Order Structures. Chemical Reviews 2017, 117 (20) , 12584-12640. https://doi.org/10.1021/acs.chemrev.6b00825
  32. Kunihiko Morihiro, Nicholas Ankenbruck, Bradley Lukasak, and Alexander Deiters . Small Molecule Release and Activation through DNA Computing. Journal of the American Chemical Society 2017, 139 (39) , 13909-13915. https://doi.org/10.1021/jacs.7b07831
  33. Kosti Tapio, Jenni Leppiniemi, Boxuan Shen, Vesa P. Hytönen, Wolfgang Fritzsche, and J. Jussi Toppari . Toward Single Electron Nanoelectronics Using Self-Assembled DNA Structure. Nano Letters 2016, 16 (11) , 6780-6786. https://doi.org/10.1021/acs.nanolett.6b02378
  34. Aradhana Chopra, Swati Krishnan, and Friedrich C. Simmel . Electrotransfection of Polyamine Folded DNA Origami Structures. Nano Letters 2016, 16 (10) , 6683-6690. https://doi.org/10.1021/acs.nanolett.6b03586
  35. Michael Matthies, Nayan P. Agarwal, and Thorsten L. Schmidt . Design and Synthesis of Triangulated DNA Origami Trusses. Nano Letters 2016, 16 (3) , 2108-2113. https://doi.org/10.1021/acs.nanolett.6b00381
  36. Dawei Jiang, Yanhong Sun, Jiang Li, Qian Li, Min Lv, Bing Zhu, Tian Tian, Dengfeng Cheng, Jiaoyun Xia, Lan Zhang, Lihua Wang, Qing Huang, Jiye Shi, and Chunhai Fan . Multiple-Armed Tetrahedral DNA Nanostructures for Tumor-Targeting, Dual-Modality in Vivo Imaging. ACS Applied Materials & Interfaces 2016, 8 (7) , 4378-4384. https://doi.org/10.1021/acsami.5b10792
  37. Benjamin Kick, Florian Praetorius, Hendrik Dietz, and Dirk Weuster-Botz . Efficient Production of Single-Stranded Phage DNA as Scaffolds for DNA Origami. Nano Letters 2015, 15 (7) , 4672-4676. https://doi.org/10.1021/acs.nanolett.5b01461
  38. Chor Yong Tay, Liang Yuan, and David Tai Leong . Nature-Inspired DNA Nanosensor for Real-Time in Situ Detection of mRNA in Living Cells. ACS Nano 2015, 9 (5) , 5609-5617. https://doi.org/10.1021/acsnano.5b01954
  39. Samet Kocabey, Susanne Kempter, Jonathan List, Yongzheng Xing, Wooli Bae, Daniel Schiffels, William M. Shih, Friedrich C. Simmel, and Tim Liedl . Membrane-Assisted Growth of DNA Origami Nanostructure Arrays. ACS Nano 2015, 9 (4) , 3530-3539. https://doi.org/10.1021/acsnano.5b00161
  40. Yuki Suzuki, Masayuki Endo, and Hiroshi Sugiyama . Mimicking Membrane-Related Biological Events by DNA Origami Nanotechnology. ACS Nano 2015, 9 (4) , 3418-3420. https://doi.org/10.1021/acsnano.5b01723
  41. Ravish Malik, Jan Genzer, and Carol K. Hall . Proteinlike Copolymers as Encapsulating Agents for Small-Molecule Solutes. Langmuir 2015, 31 (11) , 3518-3526. https://doi.org/10.1021/acs.langmuir.5b00032
  42. Jaeseung Hahn, Shelley F. J. Wickham, William M. Shih, and Steven D. Perrault . Addressing the Instability of DNA Nanostructures in Tissue Culture. ACS Nano 2014, 8 (9) , 8765-8775. https://doi.org/10.1021/nn503513p
  43. Martin Langecker, Vera Arnaut, Jonathan List, and Friedrich C. Simmel . DNA Nanostructures Interacting with Lipid Bilayer Membranes. Accounts of Chemical Research 2014, 47 (6) , 1807-1815. https://doi.org/10.1021/ar500051r
  44. Swarup Dey, Chunhai Fan, Kurt V. Gothelf, Jiang Li, Chenxiang Lin, Longfei Liu, Na Liu, Minke A. D. Nijenhuis, Barbara Saccà, Friedrich C. Simmel, Hao Yan, Pengfei Zhan. DNA origami. Nature Reviews Methods Primers 2021, 1 (1) https://doi.org/10.1038/s43586-020-00009-8
  45. Sisi Jia, Siew Cheng Phua, Yuta Nihongaki, Yizeng Li, Michael Pacella, Yi Li, Abdul M. Mohammed, Sean Sun, Takanari Inoue, Rebecca Schulman. Growth and site-specific organization of micron-scale biomolecular devices on living mammalian cells. Nature Communications 2021, 12 (1) https://doi.org/10.1038/s41467-021-25890-z
  46. Yunqi Yang, Qinyi Lu, Chao‐Min Huang, Hongji Qian, Yunlong Zhang, Sonal Deshpande, Gaurav Arya, Yonggang Ke, Stefan Zauscher. Programmable Site‐Specific Functionalization of DNA Origami with Polynucleotide Brushes. Angewandte Chemie 2021, 133 (43) , 23429-23435. https://doi.org/10.1002/ange.202107829
  47. Yunqi Yang, Qinyi Lu, Chao‐Min Huang, Hongji Qian, Yunlong Zhang, Sonal Deshpande, Gaurav Arya, Yonggang Ke, Stefan Zauscher. Programmable Site‐Specific Functionalization of DNA Origami with Polynucleotide Brushes. Angewandte Chemie International Edition 2021, 60 (43) , 23241-23247. https://doi.org/10.1002/anie.202107829
  48. Matthew R. Lakin, Darko Stefanovic, Milan N. Stojanovic. Diverse Applications of DNAzymes in Computing and Nanotechnology. 2021,,, 633-660. https://doi.org/10.1002/9783527814527.ch25
  49. Luyao Shen, Pengfei Wang, Yonggang Ke. DNA Nanotechnology‐Based Biosensors and Therapeutics. Advanced Healthcare Materials 2021, 10 (15) , 2002205. https://doi.org/10.1002/adhm.202002205
  50. Samet Kocabey, Aslihan Ekim Kocabey, Roger Schneiter, Curzio Rüegg. Membrane-Interacting DNA Nanotubes Induce Cancer Cell Death. Nanomaterials 2021, 11 (8) , 2003. https://doi.org/10.3390/nano11082003
  51. Zhiyong Zhao, Yuanchen Dong, Zongze Duan, Dou Jin, Wei Yuan, Dongsheng Liu. DNA‐organic molecular amphiphiles: Synthesis, self‐assembly, and hierarchical aggregates. Aggregate 2021, 2 (4) https://doi.org/10.1002/agt2.95
  52. Wei Yuan, Jiafang Piao, Yuanchen Dong. Advancements in the preparation methods of artificial cell membranes with lipids. Materials Chemistry Frontiers 2021, 5 (14) , 5233-5246. https://doi.org/10.1039/D1QM00501D
  53. Ankush Garg, Sharmistha Sinha. Factors deciding the assembly and thermostability of the DmrB cage. International Journal of Biological Macromolecules 2021, 182 , 959-967. https://doi.org/10.1016/j.ijbiomac.2021.04.040
  54. Bo-Young Lee, Jaewon Lee, Dong June Ahn, Seungwoo Lee, Min-Kyu Oh. Optimizing protein V untranslated region sequence in M13 phage for increased production of single-stranded DNA for origami. Nucleic Acids Research 2021, 49 (11) , 6596-6603. https://doi.org/10.1093/nar/gkab455
  55. Dong-Xia Wang, Jing Wang, Ya-Xin Wang, Yi-Chen Du, Yan Huang, An-Na Tang, Yun-Xi Cui, De-Ming Kong. DNA nanostructure-based nucleic acid probes: construction and biological applications. Chemical Science 2021, 12 (22) , 7602-7622. https://doi.org/10.1039/D1SC00587A
  56. Ruixin Li, Haorong Chen, Hyeongwoon Lee, Jong Hyun Choi. Conformational Control of DNA Origami by DNA Oligomers, Intercalators and UV Light. Methods and Protocols 2021, 4 (2) , 38. https://doi.org/10.3390/mps4020038
  57. Sofia Ojasalo, Petteri Piskunen, Boxuan Shen, Mauri A. Kostiainen, Veikko Linko. Hybrid Nanoassemblies from Viruses and DNA Nanostructures. Nanomaterials 2021, 11 (6) , 1413. https://doi.org/10.3390/nano11061413
  58. Skylar J. W. Henry, Nicholas Stephanopoulos. Functionalizing DNA nanostructures for therapeutic applications. WIREs Nanomedicine and Nanobiotechnology 2021, 14 https://doi.org/10.1002/wnan.1729
  59. Wouter Engelen, Hendrik Dietz. Advancing Biophysics Using DNA Origami. Annual Review of Biophysics 2021, 50 (1) , 469-492. https://doi.org/10.1146/annurev-biophys-110520-125739
  60. Jiafang Piao, Wei Yuan, Yuanchen Dong. Recent Progress of DNA Nanostructures on Amphiphilic Membranes. Macromolecular Bioscience 2021, 21 (5) , 2000440. https://doi.org/10.1002/mabi.202000440
  61. Shuoxing Jiang, Zhilei Ge, Shan Mou, Hao Yan, Chunhai Fan. Designer DNA nanostructures for therapeutics. Chem 2021, 7 (5) , 1156-1179. https://doi.org/10.1016/j.chempr.2020.10.025
  62. Hui Li, Kaiming Zhang, Daniel W. Binzel, Lyudmila S. Shlyakhtenko, Yuri L. Lyubchenko, Wah Chiu, Peixuan Guo. RNA nanotechnology to build a dodecahedral genome of single-stranded RNA virus. RNA Biology 2021, 141 , 1-11. https://doi.org/10.1080/15476286.2021.1915620
  63. Jorieke Weiden, Maartje M.C. Bastings. DNA origami nanostructures for controlled therapeutic drug delivery. Current Opinion in Colloid & Interface Science 2021, 52 , 101411. https://doi.org/10.1016/j.cocis.2020.101411
  64. Xue Li, Fan Xu, Donglei Yang, Pengfei Wang. A DNA-binding, albumin-targeting fusion protein promotes the cellular uptake and bioavailability of framework DNA nanostructures. Nanoscale 2021, 13 (12) , 6038-6042. https://doi.org/10.1039/D0NR07967G
  65. Youli Jia, Liman Chen, Jin Liu, Wei Li, Hongzhou Gu. DNA-catalyzed efficient production of single-stranded DNA nanostructures. Chem 2021, 7 (4) , 959-981. https://doi.org/10.1016/j.chempr.2020.12.001
  66. Arun Richard Chandrasekaran. Nuclease resistance of DNA nanostructures. Nature Reviews Chemistry 2021, 5 (4) , 225-239. https://doi.org/10.1038/s41570-021-00251-y
  67. Yang Yang, Zhenyong Wu, Laurie Wang, Kaifeng Zhou, Kai Xia, Qiancheng Xiong, Longfei Liu, Zhao Zhang, Edwin R. Chapman, Yong Xiong, Thomas J. Melia, Erdem Karatekin, Hongzhou Gu, Chenxiang Lin. Sorting sub-150-nm liposomes of distinct sizes by DNA-brick-assisted centrifugation. Nature Chemistry 2021, 13 (4) , 335-342. https://doi.org/10.1038/s41557-021-00667-5
  68. Lizhi Dai, Peng Liu, Xiaoxue Hu, Xiaozhi Zhao, Guoqiang Shao, Ye Tian. DNA origami: an outstanding platform for functions in nanophotonics and cancer therapy. The Analyst 2021, 146 (6) , 1807-1819. https://doi.org/10.1039/D0AN02160A
  69. Jiang Li, Chunhai Fan. Programming Molecular Circuitry and Intracellular Computing with Framework Nucleic Acids. 2021,,, 77-103. https://doi.org/10.1002/9783527825424.ch6
  70. Dhiraj Bhatia, Christian Wunder, Ludger Johannes. Self‐assembled, Programmable DNA Nanodevices for Biological and Biomedical Applications. ChemBioChem 2021, 22 (5) , 763-778. https://doi.org/10.1002/cbic.202000372
  71. Wen Shang Saw, Theebaa Anasamy, Yiing Yee Foo, Yee Chu Kwa, Chin Siang Kue, Chai Hong Yeong, Lik Voon Kiew, Hong Boon Lee, Lip Yong Chung. Delivery of Nanoconstructs in Cancer Therapy: Challenges and Therapeutic Opportunities. Advanced Therapeutics 2021, 4 (3) , 2000206. https://doi.org/10.1002/adtp.202000206
  72. Youngdo Yoon, Chungha Lee, Seung Joong Kim. Structural DNA nanotechnology towards therapeutic applications. Journal of the Korean Physical Society 2021, 78 (5) , 343-350. https://doi.org/10.1007/s40042-020-00049-z
  73. Daniel Fu, John Reif. 3D DNA Nanostructures: The Nanoscale Architect. Applied Sciences 2021, 11 (6) , 2624. https://doi.org/10.3390/app11062624
  74. Michael Scheckenbach, Tom Schubert, Carsten Forthmann, Viktorija Glembockyte, Philip Tinnefeld. Selbstregeneration und Selbstheilung in DNA‐Origami‐Nanostrukturen. Angewandte Chemie 2021, 133 (9) , 4982-4990. https://doi.org/10.1002/ange.202012986
  75. Michael Scheckenbach, Tom Schubert, Carsten Forthmann, Viktorija Glembockyte, Philip Tinnefeld. Self‐Regeneration and Self‐Healing in DNA Origami Nanostructures. Angewandte Chemie International Edition 2021, 60 (9) , 4931-4938. https://doi.org/10.1002/anie.202012986
  76. Henri G. Franquelim, Hendrik Dietz, Petra Schwille. Reversible membrane deformations by straight DNA origami filaments. Soft Matter 2021, 17 (2) , 276-287. https://doi.org/10.1039/D0SM00150C
  77. Xiangang Huang, Nicholas Thomas Blum, Jing Lin, Jinjun Shi, Chuan Zhang, Peng Huang. Chemotherapeutic drug–DNA hybrid nanostructures for anti-tumor therapy. Materials Horizons 2021, 8 (1) , 78-101. https://doi.org/10.1039/D0MH00715C
  78. Sofia Julin, Nonappa, Boxuan Shen, Veikko Linko, Mauri A. Kostiainen. DNA‐Origami‐Templated Growth of Multilamellar Lipid Assemblies. Angewandte Chemie International Edition 2021, 60 (2) , 827-833. https://doi.org/10.1002/anie.202006044
  79. Sofia Julin, Nonappa, Boxuan Shen, Veikko Linko, Mauri A. Kostiainen. DNA‐Origami‐Templated Growth of Multilamellar Lipid Assemblies. Angewandte Chemie 2021, 133 (2) , 840-846. https://doi.org/10.1002/ange.202006044
  80. V. Dilna, Chinnu Sabu, K. Pramod. DNA-Based Nanopharmaceuticals. 2021,,, 159-179. https://doi.org/10.1007/978-3-030-44925-4_4
  81. Celal Ulger, Esra Örenlili Yaylagül, Aykut Bilir, Necdet Saglam. Recent Advances in Nanomaterial-Based Diagnosis and Treatment: Next-Generation Sequencing, Microarray and DNA Origami. 2021,,, 135-146. https://doi.org/10.1007/978-3-030-64410-9_7
  82. Marina A. Dobrovolskaia, Mark Bathe. Opportunities and challenges for the clinical translation of structured DNA assemblies as gene therapeutic delivery and vaccine vectors. WIREs Nanomedicine and Nanobiotechnology 2021, 13 (1) https://doi.org/10.1002/wnan.1657
  83. Qihang Zhou, Xiao Wang, Changjun Zhou. DNA Design Based on Improved Ant Colony Optimization Algorithm With Bloch Sphere. IEEE Access 2021, 9 , 104513-104521. https://doi.org/10.1109/ACCESS.2021.3094876
  84. Tao Zhang. DNA origami-based microtubule analogue. Nanotechnology 2020, 31 (50) , 50LT01. https://doi.org/10.1088/1361-6528/abb395
  85. Fay Nicolson, Akbar Ali, Moritz F. Kircher, Suchetan Pal. DNA Nanostructures and DNA‐Functionalized Nanoparticles for Cancer Theranostics. Advanced Science 2020, 7 (23) , 2001669. https://doi.org/10.1002/advs.202001669
  86. Anjali Rajwar, Vinod Morya, Sumit Kharbanda, Dhiraj Bhatia. DNA Nanodevices to Probe and Program Membrane Organization, Dynamics, and Applications. The Journal of Membrane Biology 2020, 253 (6) , 577-587. https://doi.org/10.1007/s00232-020-00154-x
  87. Shelley F. J. Wickham, Alexander Auer, Jianghong Min, Nandhini Ponnuswamy, Johannes B. Woehrstein, Florian Schueder, Maximilian T. Strauss, Jörg Schnitzbauer, Bhavik Nathwani, Zhao Zhao, Steven D. Perrault, Jaeseung Hahn, Seungwoo Lee, Maartje M. Bastings, Sarah W. Helmig, Anne Louise Kodal, Peng Yin, Ralf Jungmann, William M. Shih. Complex multicomponent patterns rendered on a 3D DNA-barrel pegboard. Nature Communications 2020, 11 (1) https://doi.org/10.1038/s41467-020-18910-x
  88. Ceren Kimna, Oliver Lieleg. Molecular micromanagement: DNA nanotechnology establishes spatio-temporal control for precision medicine. Biophysics Reviews 2020, 1 (1) , 011305. https://doi.org/10.1063/5.0033378
  89. Sriram Kumar, Alexander Pearse, Ying Liu, Rebecca E. Taylor. Modular self-assembly of gamma-modified peptide nucleic acids in organic solvent mixtures. Nature Communications 2020, 11 (1) https://doi.org/10.1038/s41467-020-16759-8
  90. Christian Heck, Dmitry Torchinsky, Gil Nifker, Felix Gularek, Yael Michaeli, Elmar Weinhold, Yuval Ebenstein. Label as you fold: methyltransferase-assisted functionalization of DNA nanostructures. Nanoscale 2020, 12 (39) , 20287-20291. https://doi.org/10.1039/D0NR03694C
  91. Mohsen Khodadadi Yazdi, Ali Taghizadeh, Mohsen Taghizadeh, Florian J. Stadler, Mehdi Farokhi, Fatemeh Mottaghitalab, Payam Zarrintaj, Joshua D. Ramsey, Farzad Seidi, Mohammad Reza Saeb, Masoud Mozafari. Agarose-based biomaterials for advanced drug delivery. Journal of Controlled Release 2020, 326 , 523-543. https://doi.org/10.1016/j.jconrel.2020.07.028
  92. Wooram Park, Heejun Shin, Bogyu Choi, Won-Kyu Rhim, Kun Na, Dong Keun Han. Advanced hybrid nanomaterials for biomedical applications. Progress in Materials Science 2020, 114 , 100686. https://doi.org/10.1016/j.pmatsci.2020.100686
  93. Fan Xu, Qing Xia, Pengfei Wang. Rationally Designed DNA Nanostructures for Drug Delivery. Frontiers in Chemistry 2020, 8 https://doi.org/10.3389/fchem.2020.00751
  94. Adrian Keller, Veikko Linko. Herausforderungen und Perspektiven von DNA‐Nanostrukturen in der Biomedizin. Angewandte Chemie 2020, 132 (37) , 15950-15966. https://doi.org/10.1002/ange.201916390
  95. Adrian Keller, Veikko Linko. Challenges and Perspectives of DNA Nanostructures in Biomedicine. Angewandte Chemie International Edition 2020, 59 (37) , 15818-15833. https://doi.org/10.1002/anie.201916390
  96. Kosti Tapio, Ilko Bald. The potential of DNA origami to build multifunctional materials. Multifunctional Materials 2020, 3 (3) , 032001. https://doi.org/10.1088/2399-7532/ab80d5
  97. Arun Richard Chandrasekaran, Ken Halvorsen. Nuclease Degradation Analysis of DNA Nanostructures Using Gel Electrophoresis. Current Protocols in Nucleic Acid Chemistry 2020, 82 (1) https://doi.org/10.1002/cpnc.115
  98. Chao Wang, Jiafang Piao, Yujie Li, Xiancheng Tian, Yuanchen Dong, Dongsheng Liu. Construction of Liposomes Mimicking Cell Membrane Structure through Frame‐Guided Assembly. Angewandte Chemie 2020, 132 (35) , 15288-15292. https://doi.org/10.1002/ange.202005334
  99. Chao Wang, Jiafang Piao, Yujie Li, Xiancheng Tian, Yuanchen Dong, Dongsheng Liu. Construction of Liposomes Mimicking Cell Membrane Structure through Frame‐Guided Assembly. Angewandte Chemie International Edition 2020, 59 (35) , 15176-15180. https://doi.org/10.1002/anie.202005334
  100. Sybil Obuobi, Kjersti Julin, Elizabeth G.A. Fredheim, Mona Johannessen, Nataša Škalko-Basnet. Liposomal delivery of antibiotic loaded nucleic acid nanogels with enhanced drug loading and synergistic anti-inflammatory activity against S. aureus intracellular infections. Journal of Controlled Release 2020, 324 , 620-632. https://doi.org/10.1016/j.jconrel.2020.06.002
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