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Comparison of Gold Nanoparticle Mediated Photoporation: Vapor Nanobubbles Outperform Direct Heating for Delivering Macromolecules in Live Cells

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Laboratory of General Biochemistry and Physical Pharmacy, Ghent University, Harelbekestraat 72, 9000 Ghent, Belgium
Centre for Nano- and Biophotonics, Ghent University, Harelbekestraat 72, 9000 Ghent, Belgium
§ Department of Molecular Biotechnology, Ghent University, 9000 Ghent, Belgium
Max-Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany
*Address correspondence to [email protected]
Cite this: ACS Nano 2014, 8, 6, 6288–6296
Publication Date (Web):May 28, 2014
https://doi.org/10.1021/nn5017742
Copyright © 2014 American Chemical Society
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Abstract

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There is a great interest in delivering macromolecular agents into living cells for therapeutic purposes, such as siRNA for gene silencing. Although substantial effort has gone into designing nonviral nanocarriers for delivering macromolecules into cells, translocation of the therapeutic molecules from the endosomes after endocytosis into the cytoplasm remains a major bottleneck. Laser-induced photoporation, especially in combination with gold nanoparticles, is an alternative physical method that is receiving increasing attention for delivering macromolecules in cells. By allowing gold nanoparticles to bind to the cell membrane, nanosized membrane pores can be created upon pulsed laser illumination. Depending on the laser energy, pores are created through either direct heating of the AuNPs or by vapor nanobubbles (VNBs) that can emerge around the AuNPs. Macromolecules in the surrounding cell medium can then diffuse through the pores directly into the cytoplasm. Here we present a systematic evaluation of both photoporation mechanisms in terms of cytotoxicity, cell loading, and siRNA transfection efficiency. We find that the delivery of macromolecules under conditions of VNBs is much more efficient than direct photothermal disturbance of the plasma membrane without any noticeable cytotoxic effect. Interestingly, by tuning the laser energy, the pore size could be changed, allowing control of the amount and size of molecules that are delivered in the cytoplasm. As only a single nanosecond laser pulse is required, we conclude that VNBs are an interesting photoporation mechanism that may prove very useful for efficient high-throughput macromolecular delivery in live cells.

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  2. Nuri Oh, Sooyeon Park, Jin Woo Kim, Ji-Ho Park. Photothermal Transfection for Effective Nonviral Genome Editing. ACS Applied Bio Materials 2021, 4 (7) , 5678-5685. https://doi.org/10.1021/acsabm.1c00465
  3. Qing Wu, Rui Peng, Yufeng Luo, Qianling Cui, Shuxian Zhu, Lidong Li. Antibacterial Activity of Porous Gold Nanocomposites via NIR Light-Triggered Photothermal and Photodynamic Effects. ACS Applied Bio Materials 2021, 4 (6) , 5071-5079. https://doi.org/10.1021/acsabm.1c00318
  4. Gail A. Vinnacombe-Willson, Naihao Chiang, Leonardo Scarabelli, Yuan Hu, Liv K. Heidenreich, Xi Li, Yao Gong, Derek T. Inouye, Timothy S. Fisher, Paul S. Weiss, Steven J. Jonas. In Situ Shape Control of Thermoplasmonic Gold Nanostars on Oxide Substrates for Hyperthermia-Mediated Cell Detachment. ACS Central Science 2020, 6 (11) , 2105-2116. https://doi.org/10.1021/acscentsci.0c01097
  5. Chuanzhen Zhao, Tianxing Man, Xiaobin Xu, Qing Yang, Wenfei Liu, Steven J. Jonas, Michael A. Teitell, Pei-Yu Chiou, Paul S. Weiss. Photothermal Intracellular Delivery Using Gold Nanodisk Arrays. ACS Materials Letters 2020, 2 (11) , 1475-1483. https://doi.org/10.1021/acsmaterialslett.0c00428
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  7. Yangcui Qu, Yanxia Zhang, Qian Yu, Hong Chen. Surface-Mediated Intracellular Delivery by Physical Membrane Disruption. ACS Applied Materials & Interfaces 2020, 12 (28) , 31054-31078. https://doi.org/10.1021/acsami.0c06978
  8. Peiyuan Kang, Xiaoqing Li, Yaning Liu, Stephanie I. Shiers, Hejian Xiong, Monica Giannotta, Elisabetta Dejana, Theodore John Price, Jaona Randrianalisoa, Steven O. Nielsen, Zhenpeng Qin. Transient Photoinactivation of Cell Membrane Protein Activity without Genetic Modification by Molecular Hyperthermia. ACS Nano 2019, 13 (11) , 12487-12499. https://doi.org/10.1021/acsnano.9b01993
  9. Tianxing Man, Xiongfeng Zhu, Yu Ting Chow, Emma R. Dawson, Ximiao Wen, Alexander N. Patananan, Tingyi Leo Liu, Chuanzhen Zhao, Cong Wu, Jason S. Hong, Pei-Shan Chung, Daniel L. Clemens, Bai-Yu Lee, Paul S. Weiss, Michael A. Teitell, Pei-Yu Chiou. Intracellular Photothermal Delivery for Suspension Cells Using Sharp Nanoscale Tips in Microwells. ACS Nano 2019, 13 (9) , 10835-10844. https://doi.org/10.1021/acsnano.9b06025
  10. Wei Wang, Zhiguang Wu, Xiankun Lin, Tieyan Si, Qiang He. Gold-Nanoshell-Functionalized Polymer Nanoswimmer for Photomechanical Poration of Single-Cell Membrane. Journal of the American Chemical Society 2019, 141 (16) , 6601-6608. https://doi.org/10.1021/jacs.8b13882
  11. Jingxian Wu, Yanjun Zheng, Shuaibing Jiang, Yangcui Qu, Ting Wei, Wenjun Zhan, Lei Wang, Qian Yu, Hong Chen. Two-in-One Platform for High-Efficiency Intracellular Delivery and Cell Harvest: When a Photothermal Agent Meets a Thermoresponsive Polymer. ACS Applied Materials & Interfaces 2019, 11 (13) , 12357-12366. https://doi.org/10.1021/acsami.9b01586
  12. Siqi Wang, Lei Fu, Yong Zhang, Jing Wang, Zhenxi Zhang. Quantitative Evaluation and Optimization of Photothermal Bubble Generation around Overheated Nanoparticles Excited by Pulsed Lasers. The Journal of Physical Chemistry C 2018, 122 (42) , 24421-24435. https://doi.org/10.1021/acs.jpcc.8b07672
  13. Gaëlle Houthaeve, Ranhua Xiong, Joke Robijns, Bert Luyckx, Yasmine Beulque, Toon Brans, Coen Campsteijn, Sangram K. Samal, Stephan Stremersch, Stefaan C. De Smedt, Kevin Braeckmans, Winnok H. De Vos. Targeted Perturbation of Nuclear Envelope Integrity with Vapor Nanobubble-Mediated Photoporation. ACS Nano 2018, 12 (8) , 7791-7802. https://doi.org/10.1021/acsnano.8b01860
  14. Martin P. Stewart, Robert Langer, Klavs F. Jensen. Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts. Chemical Reviews 2018, 118 (16) , 7409-7531. https://doi.org/10.1021/acs.chemrev.7b00678
  15. Junyeob Song, Wei Zhou. Multiresonant Composite Optical Nanoantennas by Out-of-plane Plasmonic Engineering. Nano Letters 2018, 18 (7) , 4409-4416. https://doi.org/10.1021/acs.nanolett.8b01467
  16. Saya Otake, Kou Okuro, Davide Bochicchio, Giovanni M. Pavan, Takuzo Aida. Nitrobenzoxadiazole-Appended Cell Membrane Modifiers for Efficient Optoporation with Noncoherent Light. Bioconjugate Chemistry 2018, 29 (6) , 2068-2073. https://doi.org/10.1021/acs.bioconjchem.8b00270
  17. Andrea Torchi, Federica Simonelli, Riccardo Ferrando, and Giulia Rossi . Local Enhancement of Lipid Membrane Permeability Induced by Irradiated Gold Nanoparticles. ACS Nano 2017, 11 (12) , 12553-12561. https://doi.org/10.1021/acsnano.7b06690
  18. Afroditi Maria Zaki and Paola Carbone . How the Incorporation of Pluronic Block Copolymers Modulates the Response of Lipid Membranes to Mechanical Stress. Langmuir 2017, 33 (46) , 13284-13294. https://doi.org/10.1021/acs.langmuir.7b02244
  19. Sebastian Salassi, Federica Simonelli, Davide Bochicchio, Riccardo Ferrando, and Giulia Rossi . Au Nanoparticles in Lipid Bilayers: A Comparison between Atomistic and Coarse-Grained Models. The Journal of Physical Chemistry C 2017, 121 (20) , 10927-10935. https://doi.org/10.1021/acs.jpcc.6b12148
  20. Nabiha Saklayen, Marinus Huber, Marinna Madrid, Valeria Nuzzo, Daryl I. Vulis, Weilu Shen, Jeffery Nelson, Arthur A. McClelland, Alexander Heisterkamp, and Eric Mazur . Intracellular Delivery Using Nanosecond-Laser Excitation of Large-Area Plasmonic Substrates. ACS Nano 2017, 11 (4) , 3671-3680. https://doi.org/10.1021/acsnano.6b08162
  21. Hana Tarábková and Pavel Janda . Single-Step Nanoporation of Water-Immersed Polystyrene Film by Gaseous Nanobubbles. Langmuir 2016, 32 (43) , 11221-11229. https://doi.org/10.1021/acs.langmuir.6b01645
  22. Ranhua Xiong, Freya Joris, Sayuan Liang, Riet De Rycke, Saskia Lippens, Jo Demeester, Andre Skirtach, Koen Raemdonck, Uwe Himmelreich, Stefaan C. De Smedt, and Kevin Braeckmans . Cytosolic Delivery of Nanolabels Prevents Their Asymmetric Inheritance and Enables Extended Quantitative in Vivo Cell Imaging. Nano Letters 2016, 16 (10) , 5975-5986. https://doi.org/10.1021/acs.nanolett.6b01411
  23. Miao Li, Theobald Lohmüller, and Jochen Feldmann . Optical Injection of Gold Nanoparticles into Living Cells. Nano Letters 2015, 15 (1) , 770-775. https://doi.org/10.1021/nl504497m
  24. Gaëlle Houthaeve, Stefaan C. De Smedt, Kevin Braeckmans, Winnok H. De Vos. The cellular response to plasma membrane disruption for nanomaterial delivery. Nano Convergence 2022, 9 (1) https://doi.org/10.1186/s40580-022-00298-7
  25. Jelter Van Hoeck, Kevin Braeckmans, Stefaan C. De Smedt, Koen Raemdonck. Non-viral siRNA delivery to T cells: Challenges and opportunities in cancer immunotherapy. Biomaterials 2022, 26 , 121510. https://doi.org/10.1016/j.biomaterials.2022.121510
  26. Félix Sauvage, Van Phuc Nguyen, Yanxiu Li, Aranit Harizaj, J. Sebag, Dimitri Roels, Viktor Van Havere, Karen Peynshaert, Ranhua Xiong, Juan C. Fraire, Marie-José Tassignon, Katrien Remaut, Yannis M. Paulus, Kevin Braeckmans, Stefaan C. De Smedt. Laser-induced nanobubbles safely ablate vitreous opacities in vivo. Nature Nanotechnology 2022, 75 https://doi.org/10.1038/s41565-022-01086-4
  27. Jelter Van Hoeck, Christian Vanhove, Stefaan C. De Smedt, Koen Raemdonck. Non-invasive cell-tracking methods for adoptive T cell therapies. Drug Discovery Today 2022, 27 (3) , 793-807. https://doi.org/10.1016/j.drudis.2021.10.012
  28. Wrenit Gem Pearl, Elena V. Perevedentseva, Artashes V. Karmenyan, Vitaly A. Khanadeev, Sheng‐Yun Wu, Yuan‐Ron Ma, Nikolai G. Khlebtsov, Chia‐Liang Cheng. Multifunctional plasmonic gold nanostars for cancer diagnostic and therapeutic applications. Journal of Biophotonics 2022, 15 (3) https://doi.org/10.1002/jbio.202100264
  29. Ashwini Shinde, Srabani Kar, Moeto Nagai, Fan-Gang Tseng, Tuhin Subhra Santra. Light-Induced Cellular Delivery and Analysis. 2022,,, 3-30. https://doi.org/10.1007/978-981-10-8953-4_4
  30. Gaëlle Houthaeve, Gerardo García-Díaz Barriga, Stephan Stremersch, Herlinde De Keersmaecker, Juan Fraire, Jo Vandesompele, Pieter Mestdagh, Stefaan De Smedt, Kevin Braeckmans, Winnok H. De Vos. Transient nuclear lamin A/C accretion aids in recovery from vapor nanobubble-induced permeabilisation of the plasma membrane. Cellular and Molecular Life Sciences 2022, 79 (1) https://doi.org/10.1007/s00018-021-04099-9
  31. Elke De Schutter, Jana Ramon, Benjamin Pfeuty, Caroline De Tender, Stephan Stremersch, Koen Raemdonck, Ken Op de Beeck, Wim Declercq, Franck B. Riquet, Kevin Braeckmans, Peter Vandenabeele. Plasma membrane perforation by GSDME during apoptosis-driven secondary necrosis. Cellular and Molecular Life Sciences 2022, 79 (1) https://doi.org/10.1007/s00018-021-04078-0
  32. Shuichi Hashimoto, Takayuki Uwada. Laser-Induced Bubble Generation on Excitation of Gold Nanoparticles. 2022,,, 3-20. https://doi.org/10.1007/978-981-16-7798-4_1
  33. Ester Canepa, Sebastian Salassi, Federica Simonelli, Riccardo Ferrando, Ranieri Rolandi, Chiara Lambruschini, Fabio Canepa, Silvia Dante, Annalisa Relini, Giulia Rossi. Non-disruptive uptake of anionic and cationic gold nanoparticles in neutral zwitterionic membranes. Scientific Reports 2021, 11 (1) https://doi.org/10.1038/s41598-020-80953-3
  34. Yi Xuan, Subhadip Ghatak, Andrew Clark, Zhigang Li, Savita Khanna, Dongmin Pak, Mangilal Agarwal, Sashwati Roy, Peter Duda, Chandan K. Sen. Fabrication and use of silicon hollow-needle arrays to achieve tissue nanotransfection in mouse tissue in vivo. Nature Protocols 2021, 16 (12) , 5707-5738. https://doi.org/10.1038/s41596-021-00631-0
  35. Ranhua Xiong, Dawei Hua, Jelter Van Hoeck, Dominika Berdecka, Laurens Léger, Stijn De Munter, Juan C. Fraire, Laurens Raes, Aranit Harizaj, Félix Sauvage, Glenn Goetgeluk, Melissa Pille, Jeffrey Aalders, Joke Belza, Thibaut Van Acker, Eduardo Bolea-Fernandez, Ting Si, Frank Vanhaecke, Winnok H. De Vos, Bart Vandekerckhove, Jolanda van Hengel, Koen Raemdonck, Chaobo Huang, Stefaan C. De Smedt, Kevin Braeckmans. Photothermal nanofibres enable safe engineering of therapeutic cells. Nature Nanotechnology 2021, 16 (11) , 1281-1291. https://doi.org/10.1038/s41565-021-00976-3
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  37. Piersandro Pallavicini, Giuseppe Chirico, Angelo Taglietti. Harvesting Light To Produce Heat: Photothermal Nanoparticles for Technological Applications and Biomedical Devices. Chemistry – A European Journal 2021, 1 https://doi.org/10.1002/chem.202102123
  38. Laurens Raes, Melissa Pille, Aranit Harizaj, Glenn Goetgeluk, Jelter Van Hoeck, Stephan Stremersch, Juan C. Fraire, Toon Brans, Olivier Gerrit de Jong, Roel Maas-Bakker, Enrico Mastrobattista, Pieter Vader, Stefaan C. De Smedt, Bart Vandekerckhove, Koen Raemdonck, Kevin Braeckmans. Cas9 RNP transfection by vapor nanobubble photoporation for ex vivo cell engineering. Molecular Therapy - Nucleic Acids 2021, 25 , 696-707. https://doi.org/10.1016/j.omtn.2021.08.014
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  40. Xiaofan Du, Jing Wang, Lan Chen, Zhenxi Zhang, Cuiping Yao. Delivery of Foreign Materials into Adherent Cells by Gold Nanoparticle-Mediated Photoporation. Membranes 2021, 11 (8) , 550. https://doi.org/10.3390/membranes11080550
  41. Jana Ramon, Ranhua Xiong, Stefaan C. De Smedt, Koen Raemdonck, Kevin Braeckmans. Vapor nanobubble-mediated photoporation constitutes a versatile intracellular delivery technology. Current Opinion in Colloid & Interface Science 2021, 54 , 101453. https://doi.org/10.1016/j.cocis.2021.101453
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  43. Aranit Harizaj, Mike Wels, Laurens Raes, Stephan Stremersch, Glenn Goetgeluk, Toon Brans, Bart Vandekerckhove, Félix Sauvage, Stefaan C. De Smedt, Ine Lentacker, Kevin Braeckmans. Photoporation with Biodegradable Polydopamine Nanosensitizers Enables Safe and Efficient Delivery of mRNA in Human T Cells. Advanced Functional Materials 2021, 31 (28) , 2102472. https://doi.org/10.1002/adfm.202102472
  44. Laurens Raes, Stefaan C. De Smedt, Koen Raemdonck, Kevin Braeckmans. Non-viral transfection technologies for next-generation therapeutic T cell engineering. Biotechnology Advances 2021, 49 , 107760. https://doi.org/10.1016/j.biotechadv.2021.107760
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  46. Ranhua Xiong, Ronald X. Xu, Chaobo Huang, Stefaan De Smedt, Kevin Braeckmans. Stimuli-responsive nanobubbles for biomedical applications. Chemical Society Reviews 2021, 50 (9) , 5746-5776. https://doi.org/10.1039/C9CS00839J
  47. Aranit Harizaj, Filip Van Hauwermeiren, Stephan Stremersch, Riet De Rycke, Herlinde De Keersmaecker, Toon Brans, Juan C. Fraire, Karolien Grauwen, Stefaan C. De Smedt, Ine Lentacker, Mohamed Lamkanfi, Kevin Braeckmans. Nanoparticle-sensitized photoporation enables inflammasome activation studies in targeted single cells. Nanoscale 2021, 13 (13) , 6592-6604. https://doi.org/10.1039/D0NR05067A
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  49. Chenyu Qiao, Diling Yang, Xiaohui Mao, Lei Xie, Lu Gong, Xuwen Peng, Qiongyao Peng, Tao Wang, Hao Zhang, Hongbo Zeng. Recent advances in bubble-based technologies: Underlying interaction mechanisms and applications. Applied Physics Reviews 2021, 8 (1) , 011315. https://doi.org/10.1063/5.0040331
  50. Wei Liu, Xiaoyan Dong, Yang Liu, Yan Sun. Photoresponsive materials for intensified modulation of Alzheimer's amyloid-β protein aggregation: A review. Acta Biomaterialia 2021, 123 , 93-109. https://doi.org/10.1016/j.actbio.2021.01.018
  51. Bastien Duckert, Steven Vinkx, Dries Braeken, Maarten Fauvart. Single-cell transfection technologies for cell therapies and gene editing. Journal of Controlled Release 2021, 330 , 963-975. https://doi.org/10.1016/j.jconrel.2020.10.068
  52. Ali Shakeri-Zadeh, Hajar Zareyi, Roghayeh Sheervalilou, Sophie Laurent, Habib Ghaznavi, Hadi Samadian. Gold nanoparticle-mediated bubbles in cancer nanotechnology. Journal of Controlled Release 2021, 330 , 49-60. https://doi.org/10.1016/j.jconrel.2020.12.022
  53. Aranit Harizaj, Stefaan C. De Smedt, Ine Lentacker, Kevin Braeckmans. Physical transfection technologies for macrophages and dendritic cells in immunotherapy. Expert Opinion on Drug Delivery 2021, 18 (2) , 229-247. https://doi.org/10.1080/17425247.2021.1828340
  54. Ashwini Shinde, Srabani Kar, Moeto Nagai, Fan-Gang Tseng, Tuhin Subhra Santra. Light-Induced Cellular Delivery and Analysis. 2021,,, 1-29. https://doi.org/10.1007/978-981-10-4857-9_4-1
  55. Chantelle Spiteri, Valeria Caprettini, Ciro Chiappini. Biomaterials-based approaches to model embryogenesis. Biomaterials Science 2020, 8 (24) , 6992-7013. https://doi.org/10.1039/D0BM01485K
  56. Laurens Raes, Stephan Stremersch, Juan C. Fraire, Toon Brans, Glenn Goetgeluk, Stijn De Munter, Lien Van Hoecke, Rein Verbeke, Jelter Van Hoeck, Ranhua Xiong, Xavier Saelens, Bart Vandekerckhove, Stefaan De Smedt, Koen Raemdonck, Kevin Braeckmans. Intracellular Delivery of mRNA in Adherent and Suspension Cells by Vapor Nanobubble Photoporation. Nano-Micro Letters 2020, 12 (1) https://doi.org/10.1007/s40820-020-00523-0
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  58. Kavitha Illath, Ashwin Kumar Narasimhan, Pallavi Shinde, Syrpailyne Wankhar, Moeto Nagai, Tuhin Subhra Santra. Intracellular Delivery using Anisotropic Gold Nanocrystals Synthesized by Microfluidic Device. 2020,,, 448-452. https://doi.org/10.1109/NEMS50311.2020.9265617
  59. Wei Wang, Zhiguang Wu, Qiang He. Swimming nanorobots for opening a cell membrane mechanically. View 2020, 1 (3) , 20200005. https://doi.org/10.1002/VIW.20200005
  60. Sebastian Salassi, Annalisa Cardellini, Pietro Asinari, Riccardo Ferrando, Giulia Rossi. Water dynamics affects thermal transport at the surface of hydrophobic and hydrophilic irradiated nanoparticles. Nanoscale Advances 2020, 2 (8) , 3181-3190. https://doi.org/10.1039/D0NA00094A
  61. Tuhin Subhra Santra, Srabani Kar, Te-Chang Chen, Chih-Wei Chen, Jayant Borana, Ming-Chang Lee, Fan-Gang Tseng. Near-infrared nanosecond-pulsed laser-activated highly efficient intracellular delivery mediated by nano-corrugated mushroom-shaped gold-coated polystyrene nanoparticles. Nanoscale 2020, 12 (22) , 12057-12067. https://doi.org/10.1039/D0NR01792B
  62. Gen He, Ning Hu, Alexander M. Xu, Xiangling Li, Yunlong Zhao, Xi Xie. Nanoneedle Platforms: The Many Ways to Pierce the Cell Membrane. Advanced Functional Materials 2020, 30 (21) , 1909890. https://doi.org/10.1002/adfm.201909890
  63. Félix Sauvage, Joost Schymkowitz, Frederic Rousseau, Bela Z. Schmidt, Katrien Remaut, Kevin Braeckmans, Stefaan C. De Smedt. Nanomaterials to avoid and destroy protein aggregates. Nano Today 2020, 31 , 100837. https://doi.org/10.1016/j.nantod.2019.100837
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  68. Jing Liu, Chengnan Li, Toon Brans, Aranit Harizaj, Shana Van de Steene, Thomas De Beer, Stefaan De Smedt, Sabine Szunerits, Rabah Boukherroub, Ranhua Xiong, Kevin Braeckmans. Surface Functionalization with Polyethylene Glycol and Polyethyleneimine Improves the Performance of Graphene-Based Materials for Safe and Efficient Intracellular Delivery by Laser-Induced Photoporation. International Journal of Molecular Sciences 2020, 21 (4) , 1540. https://doi.org/10.3390/ijms21041540
  69. Yangcui Qu, Yanjun Zheng, Liyin Yu, Yang Zhou, Yaran Wang, Yanxia Zhang, Qian Yu, Hong Chen. A Universal Platform for High‐Efficiency “Engineering” Living Cells: Integration of Cell Capture, Intracellular Delivery of Biomolecules, and Cell Harvesting Functions. Advanced Functional Materials 2020, 30 (3) , 1906362. https://doi.org/10.1002/adfm.201906362
  70. E. Teirlinck, J.C. Fraire, H. Van Acker, J. Wille, R. Swimberghe, T. Brans, R. Xiong, M. Meire, R.J.G. De Moor, S.C. De Smedt, T. Coenye, K. Braeckmans. Laser-induced vapor nanobubbles improve diffusion in biofilms of antimicrobial agents for wound care. Biofilm 2019, 1 , 100004. https://doi.org/10.1016/j.bioflm.2019.100004
  71. Lien Van Hoecke, Laurens Raes, Stephan Stremersch, Toon Brans, Juan C. Fraire, Ria Roelandt, Wim Declercq, Peter Vandenabeele, Koen Raemdonck, Kevin Braeckmans, Xavier Saelens. Delivery of Mixed-Lineage Kinase Domain-Like Protein by Vapor Nanobubble Photoporation Induces Necroptotic-Like Cell Death in Tumor Cells. International Journal of Molecular Sciences 2019, 20 (17) , 4254. https://doi.org/10.3390/ijms20174254
  72. Laurens Raes, Clarissa Van Hecke, Julie Michiels, Stephan Stremersch, Juan C. Fraire, Toon Brans, Ranhua Xiong, Stefaan De Smedt, Linos Vandekerckhove, Koen Raemdonck, Kevin Braeckmans. Gold Nanoparticle-Mediated Photoporation Enables Delivery of Macromolecules over a Wide Range of Molecular Weights in Human CD4+ T Cells. Crystals 2019, 9 (8) , 411. https://doi.org/10.3390/cryst9080411
  73. Seied Ali Safiabadi Tali, Wei Zhou. Multiresonant plasmonics with spatial mode overlap: overview and outlook. Nanophotonics 2019, 8 (7) , 1199-1225. https://doi.org/10.1515/nanoph-2019-0088
  74. L. A. Dykman, N. G. Khlebtsov. Gold nanoparticles in chemo-, immuno-, and combined therapy: review [Invited]. Biomedical Optics Express 2019, 10 (7) , 3152. https://doi.org/10.1364/BOE.10.003152
  75. Apresio K Fajrial, Xiaoyun Ding. Advanced nanostructures for cell membrane poration. Nanotechnology 2019, 30 (26) , 264002. https://doi.org/10.1088/1361-6528/ab096b
  76. Afroditi Maria Zaki, Paola Carbone. Amphiphilic copolymers change the nature of the ordered-to-disordered phase transition of lipid membranes from discontinuous to continuous. Physical Chemistry Chemical Physics 2019, 21 (25) , 13746-13757. https://doi.org/10.1039/C9CP01293A
  77. Stephan Barcikowski, Anton Plech, Kenneth S. Suslick, Alfred Vogel. Materials synthesis in a bubble. MRS Bulletin 2019, 44 (5) , 382-391. https://doi.org/10.1557/mrs.2019.107
  78. Zhiqiang Shen, William Baker, Huilin Ye, Ying Li. pH-Dependent aggregation and pH-independent cell membrane adhesion of monolayer-protected mixed charged gold nanoparticles. Nanoscale 2019, 11 (15) , 7371-7385. https://doi.org/10.1039/C8NR09617A
  79. Mariia S. Saveleva, Karaneh Eftekhari, Anatolii Abalymov, Timothy E. L. Douglas, Dmitry Volodkin, Bogdan V. Parakhonskiy, Andre G. Skirtach. Hierarchy of Hybrid Materials—The Place of Inorganics-in-Organics in it, Their Composition and Applications. Frontiers in Chemistry 2019, 7 https://doi.org/10.3389/fchem.2019.00179
  80. Álvaro Artiga, Inés Serrano-Sevilla, Laura De Matteis, Scott G. Mitchell, Jesús M. de la Fuente. Current status and future perspectives of gold nanoparticle vectors for siRNA delivery. Journal of Materials Chemistry B 2019, 7 (6) , 876-896. https://doi.org/10.1039/C8TB02484G
  81. Timofey Pylaev, Ekaterina Vanzha, Elena Avdeeva, Boris Khlebtsov, Nikolai Khlebtsov. A novel cell transfection platform based on laser optoporation mediated by Au nanostar layers. Journal of Biophotonics 2019, 12 (1) , e201800166. https://doi.org/10.1002/jbio.201800166
  82. Jing Liu, Ranhua Xiong, Toon Brans, Saskia Lippens, Eef Parthoens, Francesca Cella Zanacchi, Raffaella Magrassi, Santosh K. Singh, Sreekumar Kurungot, Sabine Szunerits, Hannelore Bové, Marcel Ameloot, Juan C. Fraire, Eline Teirlinck, Sangram Keshari Samal, Riet De Rycke, Gaëlle Houthaeve, Stefaan C. De Smedt, Rabah Boukherroub, Kevin Braeckmans. Repeated photoporation with graphene quantum dots enables homogeneous labeling of live cells with extrinsic markers for fluorescence microscopy. Light: Science & Applications 2018, 7 (1) https://doi.org/10.1038/s41377-018-0048-3
  83. Eline Teirlinck, Ranhua Xiong, Toon Brans, Katrien Forier, Juan Fraire, Heleen Van Acker, Nele Matthijs, Riet De Rycke, Stefaan C. De Smedt, Tom Coenye, Kevin Braeckmans. Laser-induced vapour nanobubbles improve drug diffusion and efficiency in bacterial biofilms. Nature Communications 2018, 9 (1) https://doi.org/10.1038/s41467-018-06884-w
  84. Srabani Kar, Mohan Loganathan, Koyel Dey, Pallavi Shinde, Hwan-You Chang, Moeto Nagai, Tuhin Subhra Santra. Single-cell electroporation: current trends, applications and future prospects. Journal of Micromechanics and Microengineering 2018, 28 (12) , 123002. https://doi.org/10.1088/1361-6439/aae5ae
  85. Siqi Wang, Lei Fu, Jing Xin, Sijia Wang, Cuiping Yao, Zhenxi Zhang, Jing Wang. Photoacoustic response induced by nanoparticle-mediated photothermal bubbles beyond the thermal expansion for potential theranostics. Journal of Biomedical Optics 2018, 23 (12) , 1. https://doi.org/10.1117/1.JBO.23.12.125002
  86. Jen-Hung Hsiao, Yulu He, Jian-He Yu, Po-Hao Tseng, Wei-Hsiang Hua, Meng Low, Yu-Hsuan Tsai, Cheng-Jin Cai, Cheng-Che Hsieh, Yean-Woei Kiang, Chih-Chung Yang, Zhengxi Zhang. Enhancements of Cancer Cell Damage Efficiencies in Photothermal and Photodynamic Processes through Cell Perforation and Preheating with Surface Plasmon Resonance of Gold Nanoring. Molecules 2018, 23 (12) , 3157. https://doi.org/10.3390/molecules23123157
  87. Jingxian Wu, Yangcui Qu, Qian Yu, Hong Chen. Gold nanoparticle layer: a versatile nanostructured platform for biomedical applications. Materials Chemistry Frontiers 2018, 2 (12) , 2175-2190. https://doi.org/10.1039/C8QM00449H
  88. Pallavi Shinde, Loganathan Mohan, Amogh Kumar, Koyel Dey, Anjali Maddi, Alexander Patananan, Fan-Gang Tseng, Hwan-You Chang, Moeto Nagai, Tuhin Santra. Current Trends of Microfluidic Single-Cell Technologies. International Journal of Molecular Sciences 2018, 19 (10) , 3143. https://doi.org/10.3390/ijms19103143
  89. Lotte Vermeulen, Juan Fraire, Laurens Raes, Ellen De Meester, Sarah De Keulenaer, Filip Van Nieuwerburgh, Stefaan De Smedt, Katrien Remaut, Kevin Braeckmans. Photothermally Triggered Endosomal Escape and Its Influence on Transfection Efficiency of Gold-Functionalized JetPEI/pDNA Nanoparticles. International Journal of Molecular Sciences 2018, 19 (8) , 2400. https://doi.org/10.3390/ijms19082400
  90. Xin Ma, Yunfang Xiong, Leo Lee. Application of Nanoparticles for Targeting G Protein-Coupled Receptors. International Journal of Molecular Sciences 2018, 19 (7) , 2006. https://doi.org/10.3390/ijms19072006
  91. Ranhua Xiong, Peter Verstraelen, Jo Demeester, Andre G. Skirtach, Jean-Pierre Timmermans, Stefaan C. De Smedt, Winnok H. De Vos, Kevin Braeckmans. Selective Labeling of Individual Neurons in Dense Cultured Networks With Nanoparticle-Enhanced Photoporation. Frontiers in Cellular Neuroscience 2018, 12 https://doi.org/10.3389/fncel.2018.00080
  92. Shengyong Yin, Xudong Miao, Xueming Zhang, Xinhua Chen, Hao Wen. Environmental temperature affects physiology and survival of nanosecond pulsed electric field‐treated cells. Journal of Cellular Physiology 2018, 233 (2) , 1179-1190. https://doi.org/10.1002/jcp.25984
  93. Zhenjiang Zhang, Ping-Chang Lin. Noble metal nanoparticles: synthesis, and biomedical implementations. 2018,,, 177-233. https://doi.org/10.1016/B978-0-323-51254-1.00007-5
  94. Lei Wang, Jingxian Wu, Yayun Hu, Changming Hu, Yue Pan, Qian Yu, Hong Chen. Using porous magnetic iron oxide nanomaterials as a facile photoporation nanoplatform for macromolecular delivery. Journal of Materials Chemistry B 2018, 6 (27) , 4427-4436. https://doi.org/10.1039/C8TB01026A
  95. Hideya Nakamura, Satoru Watano. Direct Permeation of Nanoparticles across Cell Membrane: A Review. KONA Powder and Particle Journal 2018, 35 (0) , 49-65. https://doi.org/10.14356/kona.2018011
  96. S. Szunerits, R. Boukherroub. Near-Infrared Photothermal Heating With Gold Nanostructures. 2018,,, 500-510. https://doi.org/10.1016/B978-0-12-409547-2.13228-7
  97. Laura Wayteck, Ranhua Xiong, Kevin Braeckmans, Stefaan C. De Smedt, Koen Raemdonck. Comparing photoporation and nucleofection for delivery of small interfering RNA to cytotoxic T cells. Journal of Controlled Release 2017, 267 , 154-162. https://doi.org/10.1016/j.jconrel.2017.08.002
  98. Ranhua Xiong, Claire Drullion, Peter Verstraelen, Jo Demeester, Andre G. Skirtach, Corinne Abbadie, Winnok H. De Vos, Stefaan C. De Smedt, Kevin Braeckmans. Fast spatial-selective delivery into live cells. Journal of Controlled Release 2017, 266 , 198-204. https://doi.org/10.1016/j.jconrel.2017.09.033
  99. Nabiha Saklayen, Stefan Kalies, Marinna Madrid, Valeria Nuzzo, Marinus Huber, Weilu Shen, Jasmine Sinanan-Singh, Dag Heinemann, Alexander Heisterkamp, Eric Mazur. Analysis of poration-induced changes in cells from laser-activated plasmonic substrates. Biomedical Optics Express 2017, 8 (10) , 4756. https://doi.org/10.1364/BOE.8.004756
  100. Yamin Yang, Lei Ren, Hongjun Wang. Strategies in the design of gold nanoparticles for intracellular targeting: opportunities and challenges. Therapeutic Delivery 2017, 8 (10) , 879-897. https://doi.org/10.4155/tde-2017-0049
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