Pair your accounts.

Export articles to Mendeley

Get article recommendations from ACS based on references in your Mendeley library.

Pair your accounts.

Export articles to Mendeley

Get article recommendations from ACS based on references in your Mendeley library.

You’ve supercharged your research process with ACS and Mendeley!

STEP 1:
Click to create an ACS ID

Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

MENDELEY PAIRING EXPIRED
Your Mendeley pairing has expired. Please reconnect
ACS Publications. Most Trusted. Most Cited. Most Read
My Activity
CONTENT TYPES

Dynamic Changes in Heparan Sulfate Nanostructure in Human Pluripotent Stem Cell Differentiation

  • Deena Al Mahbuba
    Deena Al Mahbuba
    Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States
  • Sayaka Masuko
    Sayaka Masuko
    Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States
  • Shiwei Wang
    Shiwei Wang
    Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States
    McGovern Institute for Brain Research, MIT, Cambridge, Massachusetts 02139, United States
    More by Shiwei Wang
  • Deepsing Syangtan
    Deepsing Syangtan
    Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States
  • Jeong Seuk Kang
    Jeong Seuk Kang
    John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts 02139, United States
  • Yuefan Song
    Yuefan Song
    Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, United States
    More by Yuefan Song
  • Tay Won Shin
    Tay Won Shin
    Media Arts and Sciences, MIT, Cambridge, Massachusetts 02139, United States
    More by Tay Won Shin
  • Ke Xia
    Ke Xia
    Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, United States
    More by Ke Xia
  • Fuming Zhang
    Fuming Zhang
    Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, United States
    More by Fuming Zhang
  • Robert J. Linhardt
    Robert J. Linhardt
    Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, United States
  • Edward S. Boyden
    Edward S. Boyden
    McGovern Institute for Brain Research, MIT, Cambridge, Massachusetts 02139, United States
    Department of Brain and Cognitive Sciences, MIT, Cambridge, Massachusetts 02139, United States
    Media Arts and Sciences, MIT, Cambridge, Massachusetts 02139, United States
    Department of Biological Engineering, MIT, Cambridge, Massachusetts 02139, United States
    Koch Institute, MIT, Cambridge, Massachusetts 02139, United States
    Howard Hughes Medical Institute, Cambridge, Massachusetts 02139, United States
    Centers for Neurobiological Engineering and Extreme Bionics, MIT, Cambridge, Massachusetts 02139, United States
  • , and 
  • Laura L. Kiessling*
    Laura L. Kiessling
    Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States
    The Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, United States
    Koch Institute, MIT, Cambridge, Massachusetts 02139, United States
    *Phone: +1(617)258-6008. Email: [email protected]
Cite this: ACS Nano 2023, 17, 8, 7207–7218
Publication Date (Web):April 12, 2023
https://doi.org/10.1021/acsnano.2c10072
Copyright © 2023 American Chemical Society

    Article Views

    1675

    Altmetric

    -

    Citations

    LEARN ABOUT THESE METRICS
    Other access options
    Supporting Info (3)»

    Abstract

    Abstract Image

    Heparan sulfate (HS) is a heterogeneous, cell-surface polysaccharide critical for transducing signals essential for mammalian development. Imaging of signaling proteins has revealed how their localization influences their information transfer. In contrast, the contribution of the spatial distribution and nanostructure of information-rich, signaling polysaccharides like HS is not known. Using expansion microscopy (ExM), we found striking changes in HS nanostructure occur as human pluripotent stem (hPS) cells differentiate, and these changes correlate with growth factor signaling. Our imaging studies show that undifferentiated hPS cells are densely coated with HS displayed as hair-like protrusions. This ultrastructure can recruit fibroblast growth factor for signaling. When the hPS cells differentiate into the ectoderm lineage, HS is localized into dispersed puncta. This striking change in HS distribution coincides with a decrease in fibroblast growth factor binding to neural cells. While developmental variations in HS sequence were thought to be the primary driver of alterations in HS-mediated growth factor signaling, our high-resolution images indicate a role for the HS nanostructure. Our study highlights the utility of high-resolution glycan imaging using ExM. In the case of HS, we found that changes in how the polysaccharide is displayed link to profound differences in growth factor binding.

    Read this article

    To access this article, please review the available access options below.

    Get instant access

    Purchase Access

    Read this article for 48 hours. Check out below using your ACS ID or as a guest.

    Recommended

    Access through Your Institution

    You may have access to this article through your institution.

    Your institution does not have access to this content. You can change your affiliated institution below.

    Supporting Information

    ARTICLE SECTIONS
    Jump To

    The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acsnano.2c10072.

    • Video of heparan sulfate on the surface of undifferentiated hPS cells (post-expansion) (MP4)

    • Video of heparan sulfate on the surface of hPS cells-derived motor neurons (post-expansion) (MP4)

    • Figures of visualization of heparan sulfate on the cell surface of hPS with or without heparinase treatment, flow cytometry gating strategy for neural crest cells and differentiated motor neuron cells, change in heparan sulfate as hPS differentiated to neural crest cells and hMN cells, differential FGF binding to NC cells, and visualization of FGF binding to NC cells and tables of primary antibodies and qPCR primer sequences used (PDF)

    Terms & Conditions

    Electronic Supporting Information files are available without a subscription to ACS Web Editions. The American Chemical Society holds a copyright ownership interest in any copyrightable Supporting Information. Files available from the ACS website may be downloaded for personal use only. Users are not otherwise permitted to reproduce, republish, redistribute, or sell any Supporting Information from the ACS website, either in whole or in part, in either machine-readable form or any other form without permission from the American Chemical Society. For permission to reproduce, republish and redistribute this material, requesters must process their own requests via the RightsLink permission system. Information about how to use the RightsLink permission system can be found at http://pubs.acs.org/page/copyright/permissions.html.

    Cited By

    This article is cited by 1 publications.

    1. Yiran Li, Haiqi Wang, Yunlong Chen, Lin Ding, Huangxian Ju. In Situ Glycan Analysis and Editing in Living Systems. JACS Au 2024, 4 (2) , 384-401. https://doi.org/10.1021/jacsau.3c00717