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Download Hi-Res ImageDownload to MS-PowerPointCite This:Bioconjugate Chem. 2018, 29, 8, 2846-2854
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    Click Chemistry-Based DNA Labeling of Cells for Barcoding Applications
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    • Stefan D. Gentile
      Stefan D. Gentile
      Department of Bioengineering, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States
      More by Stefan D. Gentile
      Orcidhttp://orcid.org/0000-0002-5759-7157
    • Megan E. Griebel
      Megan E. Griebel
      Department of Bioengineering, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States
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    • Erik W. Anderson
      Erik W. Anderson
      Department of Bioengineering, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States
      More by Erik W. Anderson
    • Gregory H. Underhill*
      Gregory H. Underhill
      Department of Bioengineering, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States
      *E-mail: [email protected]. Phone: (217)-244-2169.
      More by Gregory H. Underhill
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    Bioconjugate Chemistry

    Cite this: Bioconjugate Chem. 2018, 29, 8, 2846–2854
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    https://doi.org/10.1021/acs.bioconjchem.8b00435
    Published July 22, 2018
    Copyright © 2018 American Chemical Society
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    Abstract

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    Cell labeling and tracking methodologies can play an important role in experiments aimed at understanding biological systems. However, many current cell labeling and tracking techniques have limitations that preclude their use in a variety of multiplexed and high-throughput applications that could best represent the heterogeneity and combinatorial complexity present in physiologic contexts. Here, we demonstrate an approach for labeling, tracking, and quantifying cells using double-stranded DNA barcodes. These barcodes are introduced to the outside of the cell membrane, giving the labeled cells a unique identifier. This approach is compatible with flow cytometric and PCR-based identification and relative quantification of the presence of barcode-labeled cells. Further, utilizing this strategy, we demonstrate the capacity for sorting and enrichment of barcoded cells from a bulk population. In addition, we illustrate the design and utility of a range of orthogonal barcode sequences, which can enable the use of multiple independent barcodes to track, sort, and enrich multiple cell types and/or cells receiving distinct treatments from a pooled sample. Overall, this method of labeling cells has the potential to track multiple populations of cells in both high-throughput in vitro and physiologic in vivo settings.

    Copyright © 2018 American Chemical Society

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    Supporting Information

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    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.bioconjchem.8b00435.

    •  Table including DNA oligo sequences utilized. Flow cytometric analysis of cell viability. Supplemental figures that demonstrate the orthogonality of the four distinct barcodes.(PDF)

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    This article is cited by 11 publications.

    1. Lu Yu, Zongrui Ma, Qunye He. Dynamic DNA Nanostructures for Cell Manipulation. ACS Biomaterials Science & Engineering 2023, 9 (2) , 562-576. https://doi.org/10.1021/acsbiomaterials.2c01204
    2. Arun Richard Chandrasekaran, Molly MacIsaac, Javier Vilcapoma, Clinton H. Hansen, Darren Yang, Wesley P. Wong, Ken Halvorsen. DNA Nanoswitch Barcodes for Multiplexed Biomarker Profiling. Nano Letters 2021, 21 (1) , 469-475. https://doi.org/10.1021/acs.nanolett.0c03929
    3. Mengyi Xiong, Qin Liu, Decui Tang, Lu Liu, Gezhi Kong, Xiaoyi Fu, Chan Yang, Yifan Lyu, Hong-Min Meng, Guoliang Ke, Xiao-Bing Zhang. “Apollo Program” in Nanoscale: Landing and Exploring Cell-Surface with DNA Nanotechnology. ACS Applied Bio Materials 2020, 3 (5) , 2723-2742. https://doi.org/10.1021/acsabm.9b01193
    4. Ruonan Liu, Yiyang Zhang, Hu Zuo, Xiao Liu, Han Yang, Lu Geng, Wen Wang, Weili Shen, Yumei Jiang, Tieqiang Sun. A novel biosensor based on antibody-controlled strand displacement amplification (SDA) and hybridization chain reaction (HCR) for tetracycline detection. Microchemical Journal 2024, 199 , 109938. https://doi.org/10.1016/j.microc.2024.109938
    5. Lexun Li, Shuang Liu, Chunjuan Zhang, Zhenzhen Guo, Shuxuan Shao, Xiaodan Deng, Qiaoling Liu. Recent Advances in DNA‐Based Cell Surface Engineering for Biological Applications. Chemistry – A European Journal 2022, 28 (69) https://doi.org/10.1002/chem.202202070
    6. Xiao Liu, Han Yang, Zehua Xu, Ruonan Liu, Hu Zuo, Zongfen Chen, Xinyang Wang, Chunyan Xia, Yuxian Zhang, Baoan Ning, Jialei Bai, Tieqiang Sun. A novel biosensor based on antibody controlled isothermal strand displacement amplification (ACISDA) system. Biosensors and Bioelectronics 2022, 209 , 114185. https://doi.org/10.1016/j.bios.2022.114185
    7. Weihong Yang, Jin Chen, Jing Yan, Shuang Liu, Yi Yan, Qiuyu Zhang. Advance of click chemistry in anion exchange membranes for energy application. Journal of Polymer Science 2022, 60 (4) , 627-649. https://doi.org/10.1002/pol.20210819
    8. Andreas Schoenit, Elisabetta Ada Cavalcanti-Adam, Kerstin Göpfrich. Functionalization of Cellular Membranes with DNA Nanotechnology. Trends in Biotechnology 2021, 39 (11) , 1208-1220. https://doi.org/10.1016/j.tibtech.2021.02.002
    9. Qunye He, Yanfei Liu, Ke Li, Yuwei Wu, Ting Wang, Yifu Tan, Ting Jiang, Xiaoqin Liu, Zhenbao Liu. Deoxyribonucleic acid anchored on cell membranes for biomedical application. Biomaterials Science 2021, 9 (20) , 6691-6717. https://doi.org/10.1039/D1BM01057C
    10. Shuxin Li, Jun Zhou, YiHui Huang, Joyita Roy, Ning Zhou, Kyungsuk Yum, Xiankai Sun, Liping Tang. Injectable Click Chemistry-based Bioadhesives for Accelerated Wound Closure. Acta Biomaterialia 2020, 110 , 95-104. https://doi.org/10.1016/j.actbio.2020.04.004
    11. Guosheng Tang, Long Chen, Zixuan Wang, Shuting Gao, Qingli Qu, Ranhua Xiong, Kevin Braeckmans, Stefaan C. De Smedt, Yu Shrike Zhang, Chaobo Huang. Faithful Fabrication of Biocompatible Multicompartmental Memomicrospheres for Digitally Color‐Tunable Barcoding. Small 2020, 16 (24) https://doi.org/10.1002/smll.201907586
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    Bioconjugate Chemistry

    Cite this: Bioconjugate Chem. 2018, 29, 8, 2846–2854
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acs.bioconjchem.8b00435
    Published July 22, 2018
    Copyright © 2018 American Chemical Society
    Request reuse permissions

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