ACS Publications. Most Trusted. Most Cited. Most Read
Real-Time Evaluation of Bacterial Viability Using Gold Nanoparticles
My Activity
    Article

    Real-Time Evaluation of Bacterial Viability Using Gold Nanoparticles
    Click to copy article linkArticle link copied!

    • Takamasa Kinoshita
      Takamasa Kinoshita
      Department of Applied Chemistry, Osaka Prefecture University, 1-2 Gakuen, Naka, Sakai, Osaka 599-8570, Japan
    • Kengo Ishiki
      Kengo Ishiki
      Department of Applied Chemistry, Osaka Prefecture University, 1-2 Gakuen, Naka, Sakai, Osaka 599-8570, Japan
      More by Kengo Ishiki
    • Dung Q. Nguyen
      Dung Q. Nguyen
      Department of Applied Chemistry, Osaka Prefecture University, 1-2 Gakuen, Naka, Sakai, Osaka 599-8570, Japan
    • Hiroshi Shiigi*
      Hiroshi Shiigi
      Department of Applied Chemistry, Osaka Prefecture University, 1-2 Gakuen, Naka, Sakai, Osaka 599-8570, Japan
      *E-mail: [email protected]
    • Tsutomu Nagaoka
      Tsutomu Nagaoka
      Department of Applied Chemistry, Osaka Prefecture University, 1-2 Gakuen, Naka, Sakai, Osaka 599-8570, Japan
    Other Access OptionsSupporting Information (1)

    Analytical Chemistry

    Cite this: Anal. Chem. 2018, 90, 6, 4098–4103
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acs.analchem.7b05439
    Published February 28, 2018
    Copyright © 2018 American Chemical Society

    Abstract

    Click to copy section linkSection link copied!
    Abstract Image

    Real-time evaluation of bacterial viability is important for various purposes such as hygiene management, development of antibacterial agents, and effective utilization of bacterial resources. Here, we demonstrate a simple procedure for evaluating bacterial viability using gold nanoparticles (Au NPs). The color of bacterial suspensions containing Au NPs strongly depended on the bacterial viability. We found that the dispersion state of Au NPs affected the color of the suspension, based on the interaction of Au NPs with substances secreted by the bacteria. This color change was easily recognized with the naked eye, and viability was accurately determined by measuring the absorbance at a specific wavelength. This method was applicable to various bacterial species, regardless of whether they were Gram-positive or Gram-negative.

    Copyright © 2018 American Chemical Society

    Read this article

    To access this article, please review the available access options below.

    Get instant access

    Purchase Access

    Read this article for 48 hours. Check out below using your ACS ID or as a guest.

    Recommended

    Access through Your Institution

    You may have access to this article through your institution.

    Your institution does not have access to this content. Add or change your institution or let them know you’d like them to include access.

    Supporting Information

    Click to copy section linkSection link copied!

    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.analchem.7b05439.

    • Confirmation of bacterial viability, other supplementing spectral data, and microscopic images (PDF)

    Terms & Conditions

    Electronic Supporting Information files are available without a subscription to ACS Web Editions. The American Chemical Society holds a copyright ownership interest in any copyrightable Supporting Information. Files available from the ACS website may be downloaded for personal use only. Users are not otherwise permitted to reproduce, republish, redistribute, or sell any Supporting Information from the ACS website, either in whole or in part, in either machine-readable form or any other form without permission from the American Chemical Society. For permission to reproduce, republish and redistribute this material, requesters must process their own requests via the RightsLink permission system. Information about how to use the RightsLink permission system can be found at http://pubs.acs.org/page/copyright/permissions.html.

    Cited By

    Click to copy section linkSection link copied!
    Citation Statements
    Explore this article's citation statements on scite.ai

    This article is cited by 17 publications.

    1. Ying Jie Zheng, Liang Rui Zhu, Jun Jiang Luo, Hao Lin Zou, Hong Qun Luo, Nian Bing Li, Bang Lin Li. In Situ Grown Gold Nanoparticles Enable Plasmonic Staining of Bacteria for High-Performance On-Site Microbial Analysis in Drinking Water. ACS ES&T Water 2024, 4 (8) , 3600-3610. https://doi.org/10.1021/acsestwater.4c00566
    2. Hikaru Ikeda, Akira Tokonami, Shigeki Nishii, Xueling Shan, Yojiro Yamamoto, Yasuhiro Sadanaga, Zhidong Chen, Hiroshi Shiigi. Evaluation of Bacterial Activity Based on the Electrochemical Properties of Tetrazolium Salts. Analytical Chemistry 2023, 95 (33) , 12358-12364. https://doi.org/10.1021/acs.analchem.3c01871
    3. Liping Gao, Jin Wen, Zhiyang Huang, Shouri Sheng, Fugang Xu, Guangran Ma, Hongliang Tan. pH Meter-Assisted Biosensor Based on Glucose Oxidase-Conjugated Magnetic Metal–Organic Framework for On-Site Evaluation of Bacterial Contamination. ACS Applied Materials & Interfaces 2023, 15 (26) , 31224-31232. https://doi.org/10.1021/acsami.3c04377
    4. Peng Fei Gao, Gang Lei, Cheng Zhi Huang. Dark-Field Microscopy: Recent Advances in Accurate Analysis and Emerging Applications. Analytical Chemistry 2021, 93 (11) , 4707-4726. https://doi.org/10.1021/acs.analchem.0c04390
    5. Yuyeon Kim, Kwangyeong Jung, Jeehan Chang, Taejin Kwak, Youngwook Lim, Seonghak Kim, Jeonggeol Na, Jinwon Lee, Inhee Choi, Luke P. Lee, Dongchoul Kim, Taewook Kang. Active Surface Hydrophobicity Switching and Dynamic Interfacial Trapping of Microbial Cells by Metal Nanoparticles for Preconcentration and In-Plane Optical Detection. Nano Letters 2019, 19 (10) , 7449-7456. https://doi.org/10.1021/acs.nanolett.9b03163
    6. Nan Gao, Xuanxuan Wang, Jingjing Tian, Yu Wang, Guixia Ling, Peng Zhang. Chitosan/nanoclusters membrane-based sensors with antibacterial properties for rapid detection of bacterial viability and food preservation. Food Hydrocolloids 2024, 154 , 110144. https://doi.org/10.1016/j.foodhyd.2024.110144
    7. Jia Liu, Zhicheng Yu, Qingmei Chen, Li Jia. L-Tryptophan assisted construction of fluorescent and colorimetric dual-channel biosensor for detection of live Escherichia coli. Microchemical Journal 2022, 174 , 107085. https://doi.org/10.1016/j.microc.2021.107085
    8. Yan Zhou, Zefeng Wang, Yanling Peng, Feiying Wang, Le Deng. Gold Nanomaterials as a Promising Integrated Tool for Diagnosis and Treatment of Pathogenic Infections—A Review. Journal of Biomedical Nanotechnology 2021, 17 (5) , 744-770. https://doi.org/10.1166/jbn.2021.3075
    9. Fumiki Takahashi, Yuko Kazui, Hajime Miyaguchi, Takeshi Ohmori, Ryutaro Tanaka, Jiye Jin. Simple colorimetric screening of the nerve agent VX using gold nanoparticles and a hand-powered extraction device. Sensors and Actuators B: Chemical 2021, 327 , 128902. https://doi.org/10.1016/j.snb.2020.128902
    10. Kyohei Matsui, So Tanabe, Shuyi Sun, Dung Nguyen, Takamasa Kinoshita, Yojiro Yamamoto, Hiroshi Shiigi. Development of Metal Nanoparticle-immobilized Microplate for High-throughput and Highly Sensitive Fluorescence Analysis. Analytical Sciences 2020, 36 (12) , 1461-1465. https://doi.org/10.2116/analsci.20P225
    11. Jiahui Sun, Jia Huang, Antony R. Warden, Xianting Ding. Real-time detection of foodborne bacterial viability using a colorimetric bienzyme system in food and drinking water. Food Chemistry 2020, 320 , 126581. https://doi.org/10.1016/j.foodchem.2020.126581
    12. Qingmiao Zhang, Yilei Wang, Wenkun Zhang, Michael E. Hickey, Zhuangsheng Lin, Qin Tu, Jinyi Wang. In situ assembly of well-dispersed Ag nanoparticles on the surface of polylactic acid-Au@polydopamine nanofibers for antimicrobial applications. Colloids and Surfaces B: Biointerfaces 2019, 184 , 110506. https://doi.org/10.1016/j.colsurfb.2019.110506
    13. Xingxing Jiang, Shuping Liu, Minghui Yang, Avraham Rasooly. Amperometric genosensor for culture independent bacterial count. Sensors and Actuators B: Chemical 2019, 299 , 126944. https://doi.org/10.1016/j.snb.2019.126944
    14. Hidenobu Nakao, Je-Deok Kim. Simple and Rapid Method for Separating Lactic Acid Bacteria from Commercially Prepared Yogurt. Analytical Sciences 2019, 35 (10) , 1065-1067. https://doi.org/10.2116/analsci.19C008
    15. Shasha Li, Su Liu, Yicheng Xu, Rufeng Zhang, Yihan Zhao, Xiaonan Qu, Yu Wang, Jiadong Huang, Jinghua Yu. Robust and highly specific fluorescence sensing of Salmonella typhimurium based on dual-functional phi29 DNA polymerase-mediated isothermal circular strand displacement polymerization. The Analyst 2019, 144 (16) , 4795-4802. https://doi.org/10.1039/C9AN00843H
    16. Hiroshi Shiigi, Tomohiro Tomiyama, Maki Saito, Kengo Ishiki, Dung Q. Nguyen, Tatsuro Endo, Yojiro Yamamoto, Xueling Shan, Zhidong Chen, Tomoaki Nishino, Hidenobu Nakao, Tsutomu Nagaoka. Smart Golden Leaves Fabricated by Integrating Au Nanoparticles and Cellulose Nanofibers. ChemNanoMat 2019, 5 (5) , 581-585. https://doi.org/10.1002/cnma.201800643
    17. Dung Quang Nguyen, Kengo Ishiki, Maki Saito, Kota Iwamoto, Hiroshi Shiigi. Advanced Materials for Pathogenie Bacterial Sensing. 2019, 370-374. https://doi.org/10.23919/ICEP.2019.8733564

    Analytical Chemistry

    Cite this: Anal. Chem. 2018, 90, 6, 4098–4103
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acs.analchem.7b05439
    Published February 28, 2018
    Copyright © 2018 American Chemical Society

    Article Views

    2796

    Altmetric

    -

    Citations

    Learn about these metrics

    Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.

    Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.

    The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated.