Efficient Labeling of Nanocellulose for High-Resolution Fluorescence Microscopy ApplicationsClick to copy article linkArticle link copied!
- Mouhanad BabiMouhanad BabiDepartment of Chemistry and Chemical Biology, McMaster University, Hamilton, Ontario L8S 4M1, CanadaMore by Mouhanad Babi
- Ayodele FatonaAyodele FatonaDepartment of Chemistry and Chemical Biology, McMaster University, Hamilton, Ontario L8S 4M1, CanadaMore by Ayodele Fatona
- Xiang LiXiang LiDepartment of Chemistry and Chemical Biology, McMaster University, Hamilton, Ontario L8S 4M1, CanadaMore by Xiang Li
- Christine CersonChristine CersonDepartment of Chemistry and Chemical Biology, McMaster University, Hamilton, Ontario L8S 4M1, CanadaMore by Christine Cerson
- Victoria M. JarvisVictoria M. JarvisMcMaster Analytical X-ray Diffraction Facility, McMaster University, Hamilton, Ontario L8S 4M1, CanadaMore by Victoria M. Jarvis
- Tiffany Abitbol
- Jose M. Moran-Mirabal*Jose M. Moran-Mirabal*Email: [email protected]Department of Chemistry and Chemical Biology, McMaster University, Hamilton, Ontario L8S 4M1, CanadaCentre for Advanced Light Microscopy, McMaster University, Hamilton, Ontario L8S 4M1, CanadaBrockhouse Institute for Materials Research, McMaster University, Hamilton, Ontario L8S 4M1, CanadaMore by Jose M. Moran-Mirabal
Abstract
The visualization of naturally derived cellulose nanofibrils (CNFs) and nanocrystals (CNCs) within nanocomposite materials is key to the development of packaging materials, tissue culture scaffolds, and emulsifying agents, among many other applications. In this work, we develop a versatile and efficient two-step approach based on triazine and azide–alkyne click-chemistry to fluorescently label nanocelluloses with a variety of commercially available dyes. We show that this method can be used to label bacterial cellulose fibrils, plant-derived CNFs, carboxymethylated CNFs, and CNCs with Cy5 and fluorescein derivatives to high degrees of labeling using minimal amounts of dye while preserving their native morphology and crystalline structure. The ability to tune the labeling density with this method allowed us to prepare optimized samples that were used to visualize nanostructural features of cellulose through super-resolution microscopy. The efficiency, cost-effectiveness, and versatility of this method make it ideal for labeling nanocelluloses and imaging them through advanced microscopy techniques for a broad range of applications.
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