Quantification of Lactobacillus reuteri ProTectis in MRS Broth Using Attenuated Total Reflectance–Fourier Transform Infrared (ATR-FTIR) Spectroscopy and ChemometricsClick to copy article linkArticle link copied!
- Norwell Brian C. Bautista*Norwell Brian C. Bautista*Phone: (033)-315-9625. Email: [email protected]Department of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, Miagao 5023, Iloilo PhilippinesDepartment of Chemistry, Loyola Science Center, The University of Scranton, Scranton, Pennsylvania 18510, United StatesMore by Norwell Brian C. Bautista
- Gerard G. DumancasGerard G. DumancasDepartment of Chemistry, Loyola Science Center, The University of Scranton, Scranton, Pennsylvania 18510, United StatesMore by Gerard G. Dumancas
- Johnziel G. UbasJohnziel G. UbasDepartment of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, Miagao 5023, Iloilo PhilippinesMore by Johnziel G. Ubas
- Eleo Jean D. BandelingEleo Jean D. BandelingDepartment of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, Miagao 5023, Iloilo PhilippinesMore by Eleo Jean D. Bandeling
- Rhett Adrian C. SeducoRhett Adrian C. SeducoDepartment of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, Miagao 5023, Iloilo PhilippinesMore by Rhett Adrian C. Seduco
- Jay O. MartizanoJay O. MartizanoDepartment of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, Miagao 5023, Iloilo PhilippinesMore by Jay O. Martizano
- Steve P. JanagapSteve P. JanagapDepartment of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, Miagao 5023, Iloilo PhilippinesMore by Steve P. Janagap
Abstract
There is an increasing global demand for probiotics because of their numerous health benefits. However, a significant percentage of commercially available probiotic products have microbial quantities that are not in accordance with their product labels. In quantifying bacteria, the viable plate count is the standard method but is considered laborious and time-consuming. We demonstrate the use of an attenuated total reflectance–Fourier transform infrared (ATR-FTIR) spectroscopy coupled with chemometrics to quantify a pure culture of Lactobacillus reuteri (L. reuteri) ProTectis grown in deMan, Rogosa, and Sharpe broth. The chemometric partial least-squares model generated was able to accurately quantify viable plate count (VPC) (root-mean-square error of cross-validation (RMSECV) = 0.115 log CFU mL–1, root-mean-square error of prediction (RMSEP) = 0.145 log CFU mL–1, R2 = 0.982). These results provide proof of concept for this quantification technique and can potentially be developed and applied for the quantification of L. reuteri ProTectis in food products.
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