Genetically Engineered Protein-Based Bioadhesives with Programmable Material PropertiesClick to copy article linkArticle link copied!
- Juya JeonJuya JeonDepartment of Energy, Environmental & Chemical Engineering, Washington University in St. Louis, One Brookings Drive, Saint Louis, Missouri 63130, United StatesMore by Juya Jeon
- Kok Zhi LeeKok Zhi LeeDepartment of Energy, Environmental & Chemical Engineering, Washington University in St. Louis, One Brookings Drive, Saint Louis, Missouri 63130, United StatesMore by Kok Zhi Lee
- Xiaolu ZhangXiaolu ZhangDepartment of Energy, Environmental & Chemical Engineering, Washington University in St. Louis, One Brookings Drive, Saint Louis, Missouri 63130, United StatesMore by Xiaolu Zhang
- John JaegerJohn JaegerDepartment of Energy, Environmental & Chemical Engineering, Washington University in St. Louis, One Brookings Drive, Saint Louis, Missouri 63130, United StatesMore by John Jaeger
- Eugene KimEugene KimDepartment of Energy, Environmental & Chemical Engineering, Washington University in St. Louis, One Brookings Drive, Saint Louis, Missouri 63130, United StatesMore by Eugene Kim
- Jingyao LiJingyao LiDepartment of Energy, Environmental & Chemical Engineering, Washington University in St. Louis, One Brookings Drive, Saint Louis, Missouri 63130, United StatesMore by Jingyao Li
- Larisa BelaygorodLarisa BelaygorodDepartment of Surgery, Section of Vascular Surgery, Washington University of Medicine in St. Louis, 660 South Euclid Avenue, Saint Louis, Missouri 63110, United StatesMore by Larisa Belaygorod
- Batool ArifBatool ArifDepartment of Surgery, Section of Vascular Surgery, Washington University of Medicine in St. Louis, 660 South Euclid Avenue, Saint Louis, Missouri 63110, United StatesMore by Batool Arif
- Guy M. GeninGuy M. GeninNSF Science and Technology Center for Engineering MechanoBiology, Department of Mechanical Engineering & Materials Science, Washington University in St. Louis, One Brookings Drive, Saint Louis, Missouri 63130, United StatesInstitute of Materials Science and Engineering, Washington University in St. Louis, One Brookings Drive, Saint Louis, Missouri 63130, United StatesDivision of Biological & Biomedical Sciences, Washington University in St. Louis, One Brookings Drive, Saint Louis, Missouri 63130, United StatesMore by Guy M. Genin
- Marcus B. FostonMarcus B. FostonDepartment of Energy, Environmental & Chemical Engineering, Washington University in St. Louis, One Brookings Drive, Saint Louis, Missouri 63130, United StatesMore by Marcus B. Foston
- Mohamed A. ZayedMohamed A. ZayedDepartment of Surgery, Section of Vascular Surgery, Washington University of Medicine in St. Louis, 660 South Euclid Avenue, Saint Louis, Missouri 63110, United StatesDepartment of Radiology, Washington University of Medicine in St. Louis, 660 South Euclid Avenue, Saint Louis, Missouri 63110, United StatesDivision of Molecular Cell Biology, Washington University of Medicine in St. Louis, 660 South Euclid Avenue, Saint Louis, Missouri 63110, United StatesDivision of Molecular Cell Biology, Washington University of Medicine in St. Louis, 660 South Euclid Avenue, Saint Louis, Missouri 63110, United StatesVeterans Affairs St. Louis Health Care System, 915 North Grand Boulevard, St. Louis, Missouri 63106, United StatesMore by Mohamed A. Zayed
- Fuzhong Zhang*Fuzhong Zhang*Email: [email protected]. Tel.: +1 (314) 935-7671. Fax: +1 (314) 935-7211.Department of Energy, Environmental & Chemical Engineering, Washington University in St. Louis, One Brookings Drive, Saint Louis, Missouri 63130, United StatesInstitute of Materials Science and Engineering, Washington University in St. Louis, One Brookings Drive, Saint Louis, Missouri 63130, United StatesDivision of Biological & Biomedical Sciences, Washington University in St. Louis, One Brookings Drive, Saint Louis, Missouri 63130, United StatesMore by Fuzhong Zhang
Abstract
Silk-amyloid-mussel foot protein (SAM) hydrogels made from recombinant fusion proteins containing β-amyloid peptide, spider silk domain, and mussel foot protein (Mfp) are attractive bioadhesives as they display a unique combination of tunability, biocompatibility, bioabsorbability, strong cohesion, and underwater adhesion to a wide range of biological surfaces. To design tunable SAM hydrogels for tailored surgical repair applications, an understanding of the relationships between protein sequence and hydrogel properties is imperative. Here, we fabricated SAM hydrogels using fusion proteins of varying lengths of silk-amyloid repeats and Mfps to characterize their structure and properties. We found that increasing silk-amyloid repeats enhanced the hydrogel’s β-sheet content (r = 0.74), leading to higher cohesive strength and toughness. Additionally, increasing the Mfp length beyond the half-length of the full Mfp sequence (1/2 Mfp) decreased the β-sheet content (r = −0.47), but increased hydrogel surface adhesion. Among different variants, the hydrogel made of 16xKLV-2Mfp displayed a high ultimate strength of 3.0 ± 0.3 MPa, an ultimate strain of 664 ± 119%, and an attractive underwater adhesivity of 416 ± 20 kPa to porcine skin. Collectively, the sequence-structure–property relationships learned from this study will be useful to guide the design of future protein adhesives with tunable characteristics for tailored surgical applications.
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This article is cited by 2 publications.
- Aliakbar Jafari, Ahmed Al‐Ostaz, Sasan Nouranian. Recent Advances in Multifunctional Naturally Derived Bioadhesives for Tissue Engineering and Wound Management. Polymers for Advanced Technologies 2024, 35
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https://doi.org/10.1002/pat.70009
- Shri Venkatesh Subramani, Jingyao Li, Kok Zhi Lee, Natalie Fisher, Fuzhong Zhang. Blending recombinant amyloid silk proteins generates composite fibers with tunable mechanical properties. Materials Advances 2024, 5
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, 3506-3516. https://doi.org/10.1039/D3MA01056B
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