Gold Nanostars for the Detection of Foodborne Pathogens via Surface-Enhanced Raman Scattering Combined with MicrofluidicsClick to copy article linkArticle link copied!
- Laura Rodríguez-LorenzoLaura Rodríguez-LorenzoInternational Iberian Nanotechnology Laboratory, Avenida Mestre José Veiga, 4715-310 Braga, PortugalMore by Laura Rodríguez-Lorenzo
- Alejandro Garrido-MaestuAlejandro Garrido-MaestuInternational Iberian Nanotechnology Laboratory, Avenida Mestre José Veiga, 4715-310 Braga, PortugalMore by Alejandro Garrido-Maestu
- Arun K. BhuniaArun K. BhuniaMolecular Food Microbiology Laboratory, Department of Food Science, Department of Comparative Pathobiology and Purdue Institute of Inflammation, Immunology and Infectious Disease, Purdue University, West Lafayette, Indiana 47907, United StatesMore by Arun K. Bhunia
- Begoña EspiñaBegoña EspiñaInternational Iberian Nanotechnology Laboratory, Avenida Mestre José Veiga, 4715-310 Braga, PortugalMore by Begoña Espiña
- Marta PradoMarta PradoInternational Iberian Nanotechnology Laboratory, Avenida Mestre José Veiga, 4715-310 Braga, PortugalMore by Marta Prado
- Lorena DiéguezLorena DiéguezInternational Iberian Nanotechnology Laboratory, Avenida Mestre José Veiga, 4715-310 Braga, PortugalMore by Lorena Diéguez
- Sara Abalde-Cela*Sara Abalde-Cela*E-mail: [email protected]International Iberian Nanotechnology Laboratory, Avenida Mestre José Veiga, 4715-310 Braga, PortugalMore by Sara Abalde-Cela
Abstract
Herein, we demonstrated the potential of surface-enhanced Raman scattering (SERS) spectroscopy combined with microfluidics for the detection and discrimination of foodborne pathogens. SERS-tagged gold nanostars were functionalized with a monoclonal antibody specific for Listeria monocytogenes. In the presence of L. monocytogenes, a SERS signal corresponding to the SERS tag paired to the antibody was detected in real time and in continuous flow, enabling the discrimination of L. monocytogenes and Listeria innocua in just 100 s. To the best of our knowledge, this is the first time that SERS tags have been used for the in-flow detection of living organisms.
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