ACS Publications. Most Trusted. Most Cited. Most Read
My Activity
CONTENT TYPES
RETURN TO ISSUEPREVResearch ArticleNEXT

A Minimized Synthetic Carbon Fixation Cycle

  • Lu Xiao
    Lu Xiao
    CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
    University of Chinese Academy of Sciences, Beijing 100049, China
    More by Lu Xiao
  • Guoxia Liu
    Guoxia Liu
    CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
    More by Guoxia Liu
  • Fuyu Gong
    Fuyu Gong
    CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
    University of Chinese Academy of Sciences, Beijing 100049, China
    More by Fuyu Gong
  • Huawei Zhu
    Huawei Zhu
    CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
    University of Chinese Academy of Sciences, Beijing 100049, China
    More by Huawei Zhu
  • Yanping Zhang
    Yanping Zhang
    CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
  • Zhen Cai
    Zhen Cai
    CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
    More by Zhen Cai
  • , and 
  • Yin Li*
    Yin Li
    CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
    *Email: [email protected]
    More by Yin Li
Cite this: ACS Catal. 2022, 12, 1, 799–808
Publication Date (Web):December 28, 2021
https://doi.org/10.1021/acscatal.1c04151
Copyright © 2021 American Chemical Society

    Article Views

    3591

    Altmetric

    -

    Citations

    LEARN ABOUT THESE METRICS
    Other access options
    Supporting Info (1)»

    Abstract

    Abstract Image

    Natural CO2 fixation cycles usually comprise multiple reactions, which may reduce the efficiency of the cycle. Here, we report the design and experimental demonstration of a minimized synthetic CO2 fixation cycle which contains only four reactions. The cycle comprises pyruvate carboxylase, oxaloacetate acetylhydrolase, acetate-CoA ligase, and pyruvate synthase and is named the POAP cycle. The POAP cycle can condense two molecules of CO2 into one molecule of oxalate in each step at the expense of two molecules of ATP and one reducing equivalent in the form of NAD(P)H. By identifying a ferredoxin from Hydrogenobacter thermophilus that can efficiently drive the rate-limiting reductive carboxylation step, the POAP cycle can be operated at 50 °C under anaerobic conditions, reaching a CO2 fixation rate of 8.0 nmol CO2 min–1 mg–1 CO2-fixing enzymes. The design and demonstration of the POAP cycle may provide a model to study CO2 fixation in the earliest organisms.

    Read this article

    To access this article, please review the available access options below.

    Get instant access

    Purchase Access

    Read this article for 48 hours. Check out below using your ACS ID or as a guest.

    Recommended

    Access through Your Institution

    You may have access to this article through your institution.

    Your institution does not have access to this content. You can change your affiliated institution below.

    Supporting Information

    ARTICLE SECTIONS
    Jump To

    The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acscatal.1c04151.

    • Thermodynamic analysis of the POAP cycle; sources, redox potentials, and molecular weights of Fds; SDS-PAGE; temperature stability of ACSmth, PYCgst, and OAHani; pathway for converting oxalate into pyruvate; LC–MS spectrum of oxalate; list of OAH, ACS, and PYC enzymes tested in this work; and DNA sequences (PDF)

    Terms & Conditions

    Electronic Supporting Information files are available without a subscription to ACS Web Editions. The American Chemical Society holds a copyright ownership interest in any copyrightable Supporting Information. Files available from the ACS website may be downloaded for personal use only. Users are not otherwise permitted to reproduce, republish, redistribute, or sell any Supporting Information from the ACS website, either in whole or in part, in either machine-readable form or any other form without permission from the American Chemical Society. For permission to reproduce, republish and redistribute this material, requesters must process their own requests via the RightsLink permission system. Information about how to use the RightsLink permission system can be found at http://pubs.acs.org/page/copyright/permissions.html.

    Cited By

    This article is cited by 14 publications.

    1. Shota Nishikawa, Wen-Chi Yu, Tony Z. Jia, Ming-Jing He, Anna Khusnutdinova, Alexander F. Yakunin, Yin-Ru Chiang, Kosuke Fujishima, Po-Hsiang Wang. Amino Acid Self-Regenerating Cell-Free Protein Synthesis System that Feeds on PLA Plastics, CO2, Ammonium, and α-Ketoglutarate. ACS Catalysis 2024, 14 (10) , 7696-7706. https://doi.org/10.1021/acscatal.4c00992
    2. Xu-Wei Ding, Jing Rong, Ze-Peng Pan, Xin-Xin Zhu, Zhen-Yu Zhu, Qi Chen, Zhi-Jun Zhang, Jian-He Xu, Chun-Xiu Li, Gao-Wei Zheng. De Novo Multienzyme Synthetic Pathways for Lactic Acid Production. ACS Catalysis 2024, 14 (7) , 4665-4674. https://doi.org/10.1021/acscatal.3c05489
    3. Souvik Ghosh, Mathieu G. Baltussen, Nikita M. Ivanov, Rianne Haije, Miglė Jakštaitė, Tao Zhou, Wilhelm T. S. Huck. Exploring Emergent Properties in Enzymatic Reaction Networks: Design and Control of Dynamic Functional Systems. Chemical Reviews 2024, 124 (5) , 2553-2582. https://doi.org/10.1021/acs.chemrev.3c00681
    4. Sarah Bierbaumer, Maren Nattermann, Luca Schulz, Reinhard Zschoche, Tobias J. Erb, Christoph K. Winkler, Matthias Tinzl, Silvia M. Glueck. Enzymatic Conversion of CO2: From Natural to Artificial Utilization. Chemical Reviews 2023, 123 (9) , 5702-5754. https://doi.org/10.1021/acs.chemrev.2c00581
    5. Ranran Wu, Fei Li, Xinyu Cui, Zehua Li, Chunling Ma, Huifeng Jiang, Lingling Zhang, Yi‐Heng P. Job Zhang, Tongxin Zhao, Yanping Zhang, Yin Li, Hui Chen, Zhiguang Zhu. Enzymatic Electrosynthesis of Glycine from CO 2 and NH 3. Angewandte Chemie 2023, 135 (14) https://doi.org/10.1002/ange.202218387
    6. Ranran Wu, Fei Li, Xinyu Cui, Zehua Li, Chunling Ma, Huifeng Jiang, Lingling Zhang, Yi‐Heng P. Job Zhang, Tongxin Zhao, Yanping Zhang, Yin Li, Hui Chen, Zhiguang Zhu. Enzymatic Electrosynthesis of Glycine from CO 2 and NH 3. Angewandte Chemie International Edition 2023, 62 (14) https://doi.org/10.1002/anie.202218387
    7. Yamei Gan, Xin Meng, Cong Gao, Wei Song, Liming Liu, Xiulai Chen. Metabolic engineering strategies for microbial utilization of methanol. Engineering Microbiology 2023, 27 , 100081. https://doi.org/10.1016/j.engmic.2023.100081
    8. Mariko Teshima, Vivian Pascal Willers, Volker Sieber. Cell-free enzyme cascades — application and transition from development to industrial implementation. Current Opinion in Biotechnology 2023, 79 , 102868. https://doi.org/10.1016/j.copbio.2022.102868
    9. Jian Zhang, Liang Guo, Cong Gao, Wei Song, Jing Wu, Liming Liu, Xiulai Chen. Metabolic engineering strategies for microbial utilization of C1 feedstocks. Systems Microbiology and Biomanufacturing 2023, 3 (1) , 122-136. https://doi.org/10.1007/s43393-022-00135-2
    10. Qinhong Wang, Yiheng Zhang, Chaoguang Tian, Zhoutong Sun, Yanhe Ma. Low-carbon biosynthesis: Opportunities and challenges. Chinese Science Bulletin 2023, 369 https://doi.org/10.1360/TB-2022-1194
    11. Liwei Guo, Lichao Sun, Yi-Xin Huo. Toward bioproduction of oxo chemicals from C1 feedstocks using isobutyraldehyde as an example. Biotechnology for Biofuels and Bioproducts 2022, 15 (1) https://doi.org/10.1186/s13068-022-02178-y
    12. Víctor de Lorenzo. Environmental Galenics: large-scale fortification of extant microbiomes with engineered bioremediation agents. Philosophical Transactions of the Royal Society B: Biological Sciences 2022, 377 (1857) https://doi.org/10.1098/rstb.2021.0395
    13. Xinyi Tan, Jens Nielsen. The integration of bio-catalysis and electrocatalysis to produce fuels and chemicals from carbon dioxide. Chemical Society Reviews 2022, 51 (11) , 4763-4785. https://doi.org/10.1039/D2CS00309K
    14. Congqiang Zhang, Christoph Ottenheim, Melanie Weingarten, LiangHui Ji. Microbial Utilization of Next-Generation Feedstocks for the Biomanufacturing of Value-Added Chemicals and Food Ingredients. Frontiers in Bioengineering and Biotechnology 2022, 10 https://doi.org/10.3389/fbioe.2022.874612

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    You’ve supercharged your research process with ACS and Mendeley!

    STEP 1:
    Click to create an ACS ID

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    MENDELEY PAIRING EXPIRED
    Your Mendeley pairing has expired. Please reconnect