Rapid, Cost-Effective Peptide/Nucleic Acid-Based Platform for Therapeutic Antibody Monitoring in Clinical SamplesClick to copy article linkArticle link copied!
- Marco MocenigoMarco MocenigoUlisse BioMed Labs, Area Science Park, SS 14, km 163.5, 34149 Trieste, ItalyMolecular Genetics and Biotechnology PhD Study Programme, University of Nova Gorica, Vipavska 13, 5000 Nova Gorica, SloveniaMore by Marco Mocenigo
- Alessandro PorchettaAlessandro PorchettaDepartment of Chemistry, University of Rome, Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, ItalyMore by Alessandro Porchetta
- Marianna RossettiMarianna RossettiDepartment of Chemistry, University of Rome, Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, ItalyMore by Marianna Rossetti
- Erik BrassErik BrassUlisse BioMed Labs, Area Science Park, SS 14, km 163.5, 34149 Trieste, ItalyMore by Erik Brass
- Lucia ToniniLucia ToniniUlisse BioMed Labs, Area Science Park, SS 14, km 163.5, 34149 Trieste, ItalyMore by Lucia Tonini
- Luca PuzziLuca PuzziUlisse BioMed Labs, Area Science Park, SS 14, km 163.5, 34149 Trieste, ItalyMore by Luca Puzzi
- Elda TagliabueElda TagliabueDepartment of Research, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, ItalyMore by Elda Tagliabue
- Tiziana TriulziTiziana TriulziDepartment of Research, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, ItalyMore by Tiziana Triulzi
- Bruna MariniBruna MariniUlisse BioMed Labs, Area Science Park, SS 14, km 163.5, 34149 Trieste, ItalyMore by Bruna Marini
- Francesco Ricci*Francesco Ricci*Email: [email protected]Department of Chemistry, University of Rome, Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, ItalyMore by Francesco Ricci
- Rudy Ippodrino*Rudy Ippodrino*Email: [email protected]Ulisse BioMed Labs, Area Science Park, SS 14, km 163.5, 34149 Trieste, ItalyMore by Rudy Ippodrino
Abstract
We demonstrate here a homogeneous assay, named NanoHybrid, for monoclonal antibody quantification directly in serum samples in a single-step format. NanoHybrid is composed of both synthetic peptide nucleic acids (PNAs) and nucleic acid strands conjugated to recognition elements and optical labels and is designed to allow fast fluorescence quantification of a therapeutic antibody. More specifically, we have characterized our analytical assay for the detection of trastuzumab (Herceptin), a monoclonal antibody (mAb) drug used for breast cancer treatment and for tumors overexpressing the HER2/neu protein. We show here that NanoHybrid is capable of performing fast drug quantification directly in blood serum. The results obtained with a pool of samples from breast cancer patients under trastuzumab treatment are compared with CE-IVD ELISA (enzyme-linked immunosorbent assay) showing a good agreement (Cohen’s K = 0.729). Due to the modular nature of the NanoHybrid platform, this technology can be programmed to potentially detect and quantify any antibody for which a high-affinity recognition element has been characterized. We envision the application of NanoHybrid in a point-of-care (POC) drug monitoring system based on disposable kits for therapeutic drug management.
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