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Programmable Nucleic Acid Nanoswitches for the Rapid, Single-Step Detection of Antibodies in Bodily Fluids

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    Programmable Nucleic Acid Nanoswitches for the Rapid, Single-Step Detection of Antibodies in Bodily Fluids
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    † Department of Chemistry, University of Rome, Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy
    ‡ Ulisse BioMed S.r.l., Area Science Park, 34149 Trieste, Italy
    § Department of Molecular and Translational Medicine, Section of Microbiology, University of Brescia Medical School, Piazzale Spedali Civili 1, 25123 Brescia, Italy
    *[email protected]
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    Journal of the American Chemical Society

    Cite this: J. Am. Chem. Soc. 2018, 140, 3, 947–953
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    https://doi.org/10.1021/jacs.7b09347
    Published January 9, 2018
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    Antibody detection plays a pivotal role in the diagnosis of pathogens and monitoring the success of vaccine immunization. However, current serology techniques require multiple, time-consuming washing and incubation steps, which limit their applicability in point-of-care (POC) diagnostics and high-throughput assays. We developed here a nucleic acid nanoswitch platform able to instantaneously measure immunoglobulins of type G and E (IgG and IgE) levels directly in blood serum and other bodily fluids. The system couples the advantages of target-binding induced colocalization and nucleic acid conformational-change nanoswitches. Due to the modular nature of the recognition platform, the method can potentially be applied to the detection of any antibody for which an antigen can be conjugated to a nucleic acid strand. In this work we show the sensitive, fast and cost-effective detection of four different antibodies and demonstrate the possible use of this approach for the monitoring of antibody levels in HIV+ patients immunized with AT20 therapeutic vaccine.

    Copyright © 2018 American Chemical Society

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    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/jacs.7b09347.

    • Binding curves, control experiments and specificity tests for anti-DIG, anti-DNP and anti-HIV1 p17 antibodies detection, competitive fluorescence antibody-based detection of DNP in solution and comparison with ELISA method for Anti-AT20 antibodies detection (PDF)

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    Cite this: J. Am. Chem. Soc. 2018, 140, 3, 947–953
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    https://doi.org/10.1021/jacs.7b09347
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