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Measurement of Bovine Somatotropin (bST) and Insulin-like Growth Factor-1 (IGF-1) in Bovine Milk Using an Electrochemiluminescent Assay

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Monsanto Company, 800 North Lindbergh Boulevard, St. Louis, Missouri 63167
* Author to whom correspondence should be addressed [telephone (314) 694-7034; fax (314) 694-9058; e-mail [email protected]].
Cite this: J. Agric. Food Chem. 2008, 56, 16, 7044–7048
Publication Date (Web):August 1, 2008
Copyright © 2008 American Chemical Society

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    Bovine somatotropin (bST) and insulin-like growth factor-1 (IGF-1) are peptide hormones that are involved in the regulation of milk production in dairy cows. Because these hormones are present at extremely low concentration in fresh and processed bovine milk, a highly sensitive and specific electrochemiluminescent immunoassay (ECLIA) has been developed to better estimate the concentration of these hormones in milk. The assay employs an imager, a capture antibody bound to a carbon electrode, and a detection antibody coupled to a ruthenium label. In the presence of tripropylamine and an electric pulse, ruthenium generates light proportional to the amount of antigen bound, and the light is captured as signal by a charge-coupled device (CCD) camera. Using bovine milk as the starting matrix, 99.69% of bST and 104.79% of IGF-1 were recoverable. The limit of detection (LOD) was <5 pg/mL for bST and <1 pg/mL for IGF-1. The limit of quantification (LOQ) was <14 pg/mL for bST in milk and <2 pg/mL of IGF-1. The assay is highly specific and shows <0.2% cross-reactivity with other peptide hormones found in bovine milk such as insulin and IGF-2. These data indicate this new, ECLIA is highly sensitive and specific for estimating the concentration of bST or IGF-1 in milk.

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