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Ampulexins: A New Family of Peptides in Venom of the Emerald Jewel Wasp, Ampulex compressa

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Download Hi-Res ImageDownload to MS-PowerPointCite This:Biochemistry 2018, 57, 12, 1907-1916
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    Ampulexins: A New Family of Peptides in Venom of the Emerald Jewel Wasp, Ampulex compressa
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    • Eugene L. Moore
      Eugene L. Moore
      Departments of Entomology and Cell Biology & Neuroscience  and  Graduate Program in Biochemistry and Molecular Biology, University of California, Riverside, California 92521, United States
      More by Eugene L. Moore
    • Ryan Arvidson
      Ryan Arvidson
      Departments of Entomology and Cell Biology & Neuroscience  and  Graduate Program in Biochemistry and Molecular Biology, University of California, Riverside, California 92521, United States
      More by Ryan Arvidson
    • Christopher Banks
      Christopher Banks
      Departments of Entomology and Cell Biology & Neuroscience  and  Graduate Program in Environmental Toxicology, University of California, Riverside, California 92521, United States
      More by Christopher Banks
    • Jean Paul Urenda
      Jean Paul Urenda
      Departments of Entomology and Cell Biology & Neuroscience, University of California, Riverside, California 92521, United States
      More by Jean Paul Urenda
    • Elizabeth Duong
      Elizabeth Duong
      Departments of Entomology and Cell Biology & Neuroscience, University of California, Riverside, California 92521, United States
      More by Elizabeth Duong
    • Haroun Mohammed
      Haroun Mohammed
      Departments of Entomology and Cell Biology & Neuroscience, University of California, Riverside, California 92521, United States
      More by Haroun Mohammed
    • Michael E. Adams*
      Michael E. Adams
      Departments of Entomology and Cell Biology & Neuroscience,  Graduate Program in Biochemistry and Molecular Biology  and  Graduate Program in Environmental Toxicology, University of California, Riverside, California 92521, United States
      *E-mail: [email protected]
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      Orcidhttp://orcid.org/0000-0003-0519-6113
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    Biochemistry

    Cite this: Biochemistry 2018, 57, 12, 1907–1916
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acs.biochem.7b00916
    Published January 19, 2018
    Copyright © 2018 American Chemical Society
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    Abstract

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    The parasitoid wasp Ampulex compressa injects venom directly into the brain and subesophageal ganglion of the cockroach Periplaneta americana, inducing a 7 to 10 day lethargy termed hypokinesia. Hypokinesia presents as a significant reduction in both escape response and spontaneous walking. We examined aminergic and peptidergic components of milked venom with HPLC and MALDI-TOF mass spectrometry. HPLC coupled with electrochemical detection confirmed the presence of dopamine in milked venom, while mass spectrometry revealed that the venom gland and venom sac have distinct peptide profiles, with milked venom predominantly composed of venom sac peptides. We isolated and characterized novel α-helical, amphipathic venom sac peptides that constitute a new family of venom toxins termed ampulexins. Injection of the most abundant venom peptide, ampulexin 1, into the subesophageal ganglion of cockroaches resulted in a short-term increase in escape threshold. Neither milked venom nor venom peptides interfered with growth of Escherichia coli or Bacillus thuringiensis on agar plates, and exposure to ampulexins or milked venom did not induce cell death in Chinese hamster ovary cells (CHO-K1) or Hi5 cells (Trichoplusia ni).

    Copyright © 2018 American Chemical Society

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    Supporting Information

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    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.biochem.7b00916.

    • Image of venom gland and venom sac apparatus, sequences of novel peptides, and cell viability assays (PDF)

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    This article is cited by 10 publications.

    1. Carlos Alberto-Silva, Fernanda Calheta Vieira Portaro, Roberto Tadashi Kodama, Lais Gomes, Brenda Rufino da Silva, Felipe Assumpção da Cunha e Silva, Ken-ichi Nihei, Katsuhiro Konno. Scoliidines: Neuroprotective Peptides in Solitary Scoliid Wasp Venoms. Toxins 2024, 16 (10) , 446. https://doi.org/10.3390/toxins16100446
    2. Amit Rana, Michael E. Adams, Frederic Libersat. Parasitoid wasp venom re-programs host behavior through downmodulation of brain central complex activity and motor output. Journal of Experimental Biology 2023, 226 (3) https://doi.org/10.1242/jeb.245252
    3. Juan Carlos Guido-Patiño, Fabien Plisson. Profiling hymenopteran venom toxins: Protein families, structural landscape, biological activities, and pharmacological benefits. Toxicon: X 2022, 14 , 100119. https://doi.org/10.1016/j.toxcx.2022.100119
    4. Carlos Alberto-Silva, Fernanda Calheta Vieira Portaro, Roberto Tadashi Kodama, Halyne Queiroz Pantaleão, Hidetoshi Inagaki, Ken-ichi Nihei, Katsuhiro Konno. Comprehensive Analysis and Biological Characterization of Venom Components from Solitary Scoliid Wasp Campsomeriella annulata annulata. Toxins 2021, 13 (12) , 885. https://doi.org/10.3390/toxins13120885
    5. Ken-ichi Nihei, Steve Peigneur, Jan Tytgat, Angela B. Lange, Katsuhiro Konno. Isolation and characterization of FMRFamide-like peptides in the venoms of solitary sphecid wasps. Peptides 2021, 142 , 170575. https://doi.org/10.1016/j.peptides.2021.170575
    6. Aida Abd El-Wahed, Nermeen Yosri, Hanem H. Sakr, Ming Du, Ahmed F. M. Algethami, Chao Zhao, Ahmed H. Abdelazeem, Haroon Elrasheid Tahir, Saad H. D. Masry, Mohamed M. Abdel-Daim, Syed Ghulam Musharraf, Islam El-Garawani, Guoyin Kai, Yahya Al Naggar, Shaden A. M. Khalifa, Hesham R. El-Seedi. Wasp Venom Biochemical Components and Their Potential in Biological Applications and Nanotechnological Interventions. Toxins 2021, 13 (3) , 206. https://doi.org/10.3390/toxins13030206
    7. Carlos Alberto-Silva, Fernanda Calheta Vieira Portaro, Roberto Tadashi Kodama, Halyne Queiroz Pantaleão, Marisa Rangel, Ken-ichi Nihei, Katsuhiro Konno. Novel neuroprotective peptides in the venom of the solitary scoliid wasp Scolia decorata ventralis. Journal of Venomous Animals and Toxins including Tropical Diseases 2021, 27 https://doi.org/10.1590/1678-9199-jvatitd-2020-0171
    8. Kenneth C. Catania. Getting the Most Out of Your Zombie: Abdominal Sensors and Neural Manipulations Help Jewel Wasps Find the Roach’s Weak Spot. Brain, Behavior and Evolution 2020, 95 (3-4) , 181-202. https://doi.org/10.1159/000511548
    9. Katsuhiro Konno, Kohei Kazuma, Marisa Rangel, Joacir Stolarz-de-Oliveira, Renato Fontana, Marii Kawano, Hiroyuki Fuchino, Izumi Hide, Tadashi Yasuhara, Yoshihiro Nakata. New Mastoparan Peptides in the Venom of the Solitary Eumenine Wasp Eumenes micado. Toxins 2019, 11 (3) , 155. https://doi.org/10.3390/toxins11030155
    10. Ryan Arvidson, Maayan Kaiser, Sang Soo Lee, Jean-Paul Urenda, Christopher Dail, Haroun Mohammed, Cebrina Nolan, Songqin Pan, Jason E. Stajich, Frederic Libersat, Michael E. Adams. Parasitoid Jewel Wasp Mounts Multipronged Neurochemical Attack to Hijack a Host Brain. Molecular & Cellular Proteomics 2019, 18 (1) , 99-114. https://doi.org/10.1074/mcp.RA118.000908
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    Biochemistry

    Cite this: Biochemistry 2018, 57, 12, 1907–1916
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acs.biochem.7b00916
    Published January 19, 2018
    Copyright © 2018 American Chemical Society
    Request reuse permissions

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