Effects of Nylon Microplastic on Feeding, Lipid Accumulation, and Moulting in a Coldwater Copepod

Microplastic debris is a pervasive environmental contaminant that has the potential to impact the health of biota, although its modes of action remain somewhat unclear. The current study tested the hypothesis that exposure to fibrous and particulate microplastics would alter feeding, impacting on lipid accumulation, and normal development (e.g., growth, moulting) in an ecologically important coldwater copepod Calanus finmarchicus. Preadult copepods were incubated in seawater containing a mixed assemblage of cultured microalgae (control), with the addition of ∼50 microplastics mL–1 of nylon microplastic granules (10–30 μm) or fibers (10 × 30 μm), which are similar in shape and size to the microalgal prey. The additive chemical profiles showed the presence of stabilizers, lubricants, monomer residues, and byproducts. Prey selectivity was significantly altered in copepods exposed to nylon fibers (ANOVA, P < 0.01) resulting in a nonsignificant 40% decrease in algal ingestion rates (ANOVA, P = 0.07), and copepods exposed to nylon granules showed nonsignificant lipid accumulation (ANOVA, P = 0.62). Both microplastics triggered premature moulting in juvenile copepods (Bernoulli GLM, P < 0.01). Our results emphasize that the shape and chemical profile of a microplastic can influence its bioavailability and toxicity, drawing attention to the importance of using environmentally relevant microplastics and chemically profiling plastics used in toxicity testing.


■ INTRODUCTION
Microplastics (1 μm to 1 mm) are a pervasive and persistent environmental contaminant, impinging on freshwater, terrestrial, and marine ecosystems across the globe. 1,2 These synthetic particulates and fibers are either directly manufactured (e.g., exfoliates in personal care products), or derive from fragmentation of larger plastic debris. 3,4 It is conservatively estimated that over 4.75 × 10 12 plastic particles (in the size range of 0.3−4.5 mm) are floating in the global ocean. 5 As complete mineralization of plastic debris is estimated to range from tens to hundreds of years, and with plastic inputs expected to rise for the foreseeable future, marine microplastic concentrations are likely to increase. 6,7 Owing to their small size, microplastics can be directly or indirectly (i.e., via trophic interactions) ingested by a range of marine organisms across trophic levels, including zooplankton, 8 shellfish, 9 fish, 10,11 and megafauna. 12,13 Microplastics contain additives, plasticizers, and monomers (e.g., bisphenol A, polybrominated diphenyl ethers) incorporated during their manufacture to provide a wide range of functions including as emollients, stabilizers, and flame retardants. 14 Furthermore, they may carry persistent organic pollutants (POPs), metals, and pathogens that adsorb or adhere to their surface in the marine environment. 15,16 Where equilibrium has not been reached (i.e., there is a chemical gradient), there is evidence that POPs and metals (e.g., copper, zinc) can transfer from microplastics into biota potentially enhancing their toxicity. 17,18 Laboratory testing has highlighted the negative impacts microplastic ingestion can have on marine organisms, including zooplankton, mussels, oysters, crustaceans, and fish, with effects including reduced feeding, fecundity, growth, and survival. 19−24 These effects can cascade through levels of biological organization, resulting in impacts on the ecological functionality of keystone species (e.g., bioturbation, nutrient cycling). 25 Copepods are an abundant class of marine zooplankton that provide an essential trophic link between primary producers and secondary consumers, and contribute to ecological processes such as marine nutrient cycling. 26 Laboratory exposures have demonstrated the capacity for a range of pelagic and benthic copepods, including Acartia spp., Calanus spp, Centropages spp., Limnocalanus spp., Temora spp., and Tigriopus spp., to ingest polystyrene microplastic beads and fragments. 27−30 Furthermore, wild copepods sampled from the South China Sea 31 and Northeast Pacific Ocean 32 have been shown to ingest microplastic fibers and particulates in the natural environment. Exposure studies have highlighted that polystyrene microspheres can negatively affect copepod feeding and health. Exposed to a monoalgal diet and polystyrene beads (20 μm; 65 microplastics mL −1 ), the temperate calanoid copepod Calanus helgolandicus showed significant reductions in their dietary intake of carbon owing to a shift in feeding, with a preference for smaller, less nutritious algae. 22 After 3 days exposure, Cole et al. 22 observed microplastic exposed copepods produced significantly smaller eggs with reduced hatching success, which was attributed to reduced energetic intake. There is relatively little data currently available to determine whether microplastics with different physical properties (e.g., shape, size, density) 33 will exhibit altered bioavailability or toxicity, and there are few published microplastic exposure studies focused on early life stages. Incorporation of microplastics more representative of those found in the environment (i.e., irregularly shaped, fibers) and consideration of impacts on early life stages have been encouraged within the scientific community. 34,35 In this study, we investigate the impact of fibrous and particulate microplastic exposure on feeding, lipid accumulation, growth, and moulting in preadult Calanus f inmarchicus, a boreal (coldwater) copepod that is widely distributed in the northern hemisphere and the dominant mesozooplankton species in the North Sea and Norwegian Sea. 36 The energetic reserve of C. f inmarchicus takes the form of a large oil sac, comprising wax esters with long-chain fatty acids and fatty alcohols, built-up during their juvenile life stages in periods of high food availability. 37,38 These lipid stores make C. f inmarchicus nutritionally valuable to higher-trophic organisms (e.g., fish, whales), and facilitate "diapause", whereby the copepods descend to deeper waters and enter a state of dormancy over winter. 39,40 We hypothesized that reductions in algal feeding stemming from microplastic exposure would result in reduced lipid accumulation in developing C. f inmarchicus with consequences for the normal development of the copepod. In our experiments, cohorts of preadult C. f inmarchicus (copepodite stage, CV) were exposed to mixed algal assemblages with the addition of either nylon microplastic fibers or granules at a concentration of 50 microplastics mL −1 for 6 days, with sublethal end points including: algal ingestion rates, growth, lipid mass and profiles, and moulting. Our results provide evidence of the risks fibrous and particulate microplastics pose to the energetics and development of a keystone species.

■ MATERIALS AND METHODS
Copepods and Microalgae. Juvenile Calanus f inmarchicus (CV) were subsampled from copepod cultures maintained at the Norwegian University of Science and Technology (NTNU). Copepods were fed a mixed assemblage of three microalgal species of different size and shape (Supporting Information, SI, Figure S1), which are part of the natural diet of Calanus sp.: (i) the chlorophyte Dunaliella tertiolecta (9 × 13 μm); (ii) the chain-forming diatom Thalassiosira rotula (19 × 24 μm); and (iii) the dinoflagellate Scripsiella trochoidea (29 × 34 μm). Algae were cultured with F/2 media, with addition of silica for T. rotula, and maintained at 18°C at a 16:8 light/dark cycle. Copepods were fed a nonlimiting concentration of microalgae, comprising ∼200 cells mL −1 of D. tertiolecta, ∼50 cell mL −1 of T. rotula, and ∼15 cells mL −1 of S. trochoidea. D. tertiolecta were quantified using a Coulter Counter (Beckman Multisizer 3), while T. rotula and S. trochoidea were quantified using a Sedgewick rafter chamber. C. f inmarchicus were acclimated to their algal prey for 48 h prior to experiments. The carbon biomass of algal prey was estimated using a literature derived conversion factor of 5 nL biovolume ≈ 1 μg C. 41 To calculate algal biovolume, microalgae were imaged under an inverted microscope (Nikon TE2000S), cellular dimensions determined using ImageJ, and the formulas for a volume of an ellipsoid (D. tertiolecta and S. trochoidea) or cylinder (T. rotula) applied.
Microplastics. Nylon fibers (10 × 30 μm), of a similar shape and size as the chain-forming microalgae T. rotula, were prepared by sectioning polyamide nylon-6,6 polyfilament line (Goodfellow; AM325705) per the method of Cole (2016). 42 In brief, the polyfilament line was wrapped continuously around a custom spool, embedded in TissueTek cryogenic solution, and then sectioned at 30 μm intervals using a cryogenic microtome (LEICA CM1950). Nylon granules (10− 30 μm), of a similar shape and size distribution as the unicellular microalgae D. tertiolecta and S. trochoidea, were prepared by size fractionating polyamide nylon-6 powder (Goodfellow; AM306010) with 30 μm nylon mesh and 10 μm polycarbonate membrane filters. Prior to use, microplastics were rinsed with ethanol and copious amounts of ultrapure water, suspended in ultrapure water and quantified using a Sedgewick rafter chamber. For imaging purposes, a subsample of the fibrous and granular microplastics were dyed with Nile Red (500 μg mL −1 ).
Chemical Profiling. To ascertain what compounds (e.g., monomers, additives) were present in the microplastics, samples of fibers (∼20 mg) and granules (∼50 mg) were extracted using either 4 mL of dichloromethane (DCM, Rathburn; n = 3) or 4 mL of ethyl acetate (EtOAc, Fluka; n = 3). Solvent was added to each sample and then the sample sonicated for 30 min (Bandelin Sonorex Super RK 510H ultrasonication bath, 640W, 35 kHz) at either room temperature (DCM) or 65°C (EtOAc). The solvent extract was filtered through a pipette packed with Bilson cotton and a small amount (∼50 mg) of anhydrous Na 2 SO 4 to remove particulates, and then concentrated by solvent evaporation (40°C under a gentle flow of N 2 ) to an approximate volume of 500 μL prior to analysis by GC-MS (Agilent 7890A GC equipped with an Agilent 5975C Mass Selective Detector). Here, the inlet was set to 250°C, the transfer line to 300°C, the ion source to 230°C and the quadrupole to 150°C. The carrier gas was helium, at a constant flow of 1.1 mL/min. One μL of sample was injected by pulsed splitless injection (Agilent DB5-MS ultrainert GC column; 30 m, 0.25 μm film thickness, 0.25 mm internal diameter). The GC oven was held at 40°C (2 min), ramped by 6°C min −1 to 320°C (20 min hold). Mass spectra were recorded after 12 min of hold time (50−500

Environmental Science & Technology
Article m/z). Chromatograms and mass spectra were recorded using Chemstation software, investigated in Masshunter Qualitative Navigator B.08.00, further processed using Masshunter Unknowns Analysis ("Unknowns") followed by export to csv format using Python and data processed in R. After initial inspection of chromatograms, peaks were deconvoluted using Unknowns algorithms and best hits from the NIST 2017 library were extracted. Compounds were filtered based on observed presence in at least 3 of the 6 total replicates and >80% match to NIST 2017 library mass spectra.
Exposure. Treatments comprised: (i) controls, (ii) nylon fibers, and (iii) nylon granules. Exposure media consisted of 0.22 μm filtered natural seawater containing mixed algal prey and 20 mL of Guillard's F/2 media to ensure water remained nutrient replete, plus 50 microplastics mL −1 of nylon fibers or granules as applicable. Stocks were prepared daily, thoroughly mixed with a perforated plunger, and then carefully poured into 1 L glass bottles (n = 10 per treatment). Ten preadult copepods (copepodite stage CV) were added to each bottle, and exposure media used to fill all bottles to the brim (total volume 1150 mL), thereby eliminating air bubbles. To account for the natural growth of the algae (see Algal ingestion rates), exposure media in 500 mL bottles (n = 5) was also incubated without copepods on Day 3−4. Bottles were secured to a rotating plankton wheel (<5 rpm) submerged in a water bath for temperature stability, and the setup maintained at 8.7 ± 0.1°C in the dark for a total of 6 days. Water changes were conducted daily by gently pouring the contents of each bottle through a partially submerged 500 μm mesh to isolate copepods; on Day 3−4 media was preserved for calculation of algal ingestion rates. The developmental stage of each copepod was noted (see Moulting), and then the specimens transferred to fresh media. Any individuals damaged during water changes were removed and if experimental cohorts were reduced by >50% the replicate was rejected. At the end of the exposure, copepods were anaesthetised using FinQuel (MS222), and then photographed under a stereo microscope (Leica MZAPO/Nikon DigitalSight Fi1-U2). Individual copepods were transferred to a cryovial, snap-frozen in liquid nitrogen and subsequently stored at −80°C prior to lipid analysis.
Microplastic Uptake. To verify that juvenile C. f inmarchicus had the capacity to ingest microplastics, we conducted a 2 h exposure (per the experimental protocol described above) using nylon fibers and granules dyed with Nile Red (100 microplastics mL −1 ). Following the exposure, copepods and their faecal pellets were isolated using a 63 μm mesh, transferred to a clean glass Petri dish, and subsequently visualized and photographed under a stereo microscope (Leica MZAPO/Nikon DigitalSight Fi1-U2) equipped with a stereofluorescence module (Leica "green" fluorescent filter; excitation 546/10 nm, dichroic splitter 565 nm and emission OG590).
Algal Ingestion Rates. Feeding rates (i.e., algal ingestion rates) were assessed midway through the exposure (Day 3−4). At T 0 (Day 3), 200 mL subsamples of algal stocks were collected and preserved with 2% Lugols solution (n = 5 per treatment). After 24 h (Day 4), 200 mL subsamples were taken from all bottles (including algal controls without predation), and fixed with 2% Lugols. Preserved samples were maintained in amber glass bottles prior to analysis to prevent degradation. Microalgae were quantified using the Utermoḧl technique (BSEN15204:2006). In brief, samples were settled in 100 mL Utermoḧl chambers for 48 h, samples viewed under inverted microscope (Olympus IMT2) and cells systematically enumerated. Cell concentrations and mean carbon biomass of the microalgae were subsequently used to calculate algal ingestion rates (μg C individual −1 day −1 ) per the equation of Frost. 43 Prosome Length. The prosome length (μm) of copepods was ascertained for copepods subsampled from initial stocks (Day 0, n = 25) and juvenile, female and male copepods at the end of the 6-day exposure. In all cases, individual copepods were anaesthetised and photographed under a stereo microscope (Leica MZAPO/Nikon DigitalSight Fi1-U2), and prosome length measured using ImageJ software.
Total Lipid Mass and Lipid Profiles. Copepod lipids were extracted by adapting the protocol of Folch et al., 44 adding 500 μL of chloroform−methanol (2:1 v/v) and then placing the sample in a −20°C freezer for 4 h. Next, a phase separation of nonpolar lipids and polar organics/compounds was undertaken by adding 130 μL potassium chloride (0.88% w/w), and then vortexing (12−16 rpm), and centrifuging (2.5 min, 2500 rpm) the sample. A glass-steel pipette was used to carefully extract and transfer the bottom phase (containing nonpolar lipids) to a preweighed glass vial. The solvent was gently evaporated using nitrogen gas, and the sample desiccated under vacuum in the dark for 30 min. Finally, the vial was weighed on a mass balance to ascertain the total lipid mass (mg). To assess the lipid content of the microalgae, 1−2 mL of microalgae were filtered through a GFF; filters were placed in 7 mL glass vials, the lipids extracted using 5 mL of chloroform−methanol (2:1 v/v), and then processed as above. Prior to lipid profiling, 250 μL of chloroform was added to each vial, and samples stored at −20°C. For determination of lipid profiles, internal standards (23:0, fatty acid and 19:0 fatty alcohol) were added to the total lipid samples prior to methylation in 1% methanol for 16 h at 50°C. Fatty acid methyl esters and free fatty alcohols were purified using High Performance Thin Layer Chromatography (HPTLC) and analyzed by gas chromatography using a Thermo Trace 2000 GC equipped with a Resteck Stabilwax column.
Moulting. Every 24 h the developmental stage of copepods was determined, based upon the morphological characteristics of each copepod (SI Figure S2). For adult copepods, their sex was determined at the end of the exposure period through morphological assessment of anaesthetised specimens.
Statistical Analyses. Statistical analysis was conducted using R statistical software V 1.0.136. 45 Data were tested for normality using a Shapiro-Wilk test and homogeneity of variance was visually inspected to satisfy apriori parametric requisites. An ANOVA with posthoc Tukey tests was used to compare biological data including algal ingestion rates, prosome length, and lipid mass. A General Linear Model (GLM) was used to compare fatty acid and alcohol data. Moulting data were assembled into binary format ("Moulted = 1", "Not-moulted = 0"), and a Bernoulli GLM used to assess the probability of copepod moulting for each treatment, with homogeneity among replicates determined using "binomial" family and "logit" link functions. Model assumptions were validated by extracting deviance residuals and examining their distribution. Data are presented as mean ± standard error, with statistical significance assigned where P < 0.05.

Environmental Science & Technology
Article ■ RESULTS Microplastic Uptake. Following a 2 h exposure, nylon fibers and granules were visualized in the intestinal tracts and faecal pellets of the juvenile copepods, confirming uptake and egestion (Figure 1).
Algal Ingestion Rates. Average microalgal dimensions (SI Table S3) were used to calculate mean carbon biomass per cell values of 0.98 ng C for D. tertiolecta, 1.66 ng C for T. rotula, and 25.2 ng C for S. trochoidea. Mean microplastic concentrations in aqueous media were 46.6 fibers and 53.4 granules mL −1 ; mean ingestion rates for microplastics were ∼1700 fibers copepod −1 d −1 and ∼5700 granules copepod −1 d −1 . Copepods exposed to both fibers and granules showed a slight (nonsignificant) increase in algal ingestion rates for D. tertiolecta (ANOVA, P = 0.30; Figure 2A). Copepods exposed to nylon fibers showed significant reductions in algal ingestion rates for T. rotula and S. trochoidea (ANOVA, P < 0.01; Figure  2B/C), whereas copepods exposed to nylon granules showed no differences in feeding rates for T. rotula (ANOVA, P = 0.44) or S. trochoidea (ANOVA, P = 0.87). Overall, copepods exposed to fibers showed an average 40% reduction in algal ingestion (ANOVA, P = 0.07; Figure 2D). No difference in total algal ingestion rates were observed for copepods exposed to nylon granules (ANOVA, P = 0.88).
Total Lipid Mass and Lipid Profiles. The average lipid mass of juvenile copepods at the start of the experiment was 66.7 ± 5.5 μg. Significant lipid accumulation was observed in juvenile copepods in the control and fiber treatments (ANOVA, P < 0.01), but not the granule treatment (ANOVA, P = 0.63; Figure 3). The average lipid content of juvenile copepods in the control treatment (100.4 ± 7.0 μg) exceeded that of copepods in the granule treatment (77.2 ± 6.3 μg), however this difference was not statistically significant (ANOVA, P = 0.07; Figure 3). Furthermore, no significant differences were observed in the lipid mass of female (ANOVA, P = 0.42) or male (ANOVA, P = 0.96) copepods. There was no significant difference in juvenile copepod fatty acid (GLM, control-fibers, P = 0.09; GLM, control-granules P = 0.34; SI Figure S4) or fatty alcohol (GLM, control-fibers P = 0.80; GLM, control-granules P = 0.90; SI Figure S5) composition. For individual fatty acids and alcohols, the prevalence of palmitoleic acid (16:1 (n −7 )) and linoleic acid (18:2 (n −6 )) were significantly reduced in the fiber treatment (GLM, P < 0.05), and the prevalence of palmityl alcohol    Figure 4). The proportion of copepods reaching adulthood increased between Days 5 and 6 for all treatments. On Day 6 30.6 ± 9.5% of controls had moulted, with a higher proportion of moults in copepods exposed to nylon fibers (52.3 ± 7.5%; Bernoulli GLM, b = 0.86, z = 1.84, P = 0.07) and a statistically significant higher proportion of moults in copepods exposed to nylon granules (55.9 ± 9.9%; Bernoulli GLM, b = 1.14, z = 0.49, P < 0.05).

■ DISCUSSION
Our study reveals that microplastic exposure can impact upon prey selectivity, feeding, lipid accumulation, and moulting in a keystone marine organism. Microplastic shape influenced bioavailability and observed effects, with exposure to nylon fibers causing significant shifts in prey selectivity resulting in a 40% decrease in algal ingestion rates, and nylon granules negatively affecting lipid accumulation. We further observed that both microplastic types caused premature moulting, although the mechanism underpinning this developmental shift remains unclear. These results add to the growing evidence that at high concentrations, marine microplastics can significantly affect copepod feeding and health, with potential knock-on effects for marine food webs and ecological processes in which copepods play vital roles.
Uptake. Ingestion and egestion of both nylon microplastic fibers and granules was observed in juvenile C. f inmarchicus. The capacity for copepods and other zooplankton to ingest spherical polystyrene beads under laboratory conditions has been widely demonstrated, 34 and juvenile (CV), female and male C. f inmarchicus have been shown to readily ingest and egest polystyrene fragments (<30 μm diameter). 30 Furthermore, irregularly shaped and fibrous microplastics have been identified in wild copepods sampled from the natural environment. 31,32 Feeding. When exposed to the fibrous microplastics, juvenile C. f inmarchicus demonstrated substantial shifts in feeding, with significantly reduced ingestion rates for the largest algae T. rotula (19 × 24 μm) and S. trochoidea (29 × 34 μm), contributing to a 40% decrease in ingested biomass compared with controls. A comparable shift in feeding selectivity was observed in the temperate copepod Calanus helgolandicus, in which exposure to 20 μm polystyrene beads resulted in a preferential shift toward smaller algae, similarly resulting in a 40% reduction in ingested biomass. 22 In mixed algal assemblages C. f inmarchicus typically predate on larger, nutritionally valuable prey (e.g., diatoms, dinoflagellates, and ciliates), 46,47 for which they display higher filtration rates and feeding efficiencies. 48,49 This preference for larger algae is evident for copepods in the control and granule treatments, with >50% of ingested biomass derived from S. trochoidea. However, for copepods exposed to fibers the majority of ingested biomass came from the smallest algae, D. tertiolecta (9 × 13 μm). This shift in prey selectivity would therefore suggest copepods are avoiding microalgae of similar shape (i.e., chainforming D. tertiolecta) and size (i.e., S. trochoidea) to the nylon fibers (10 × 30 μm). This hypothesis is further supported by our finding that fibers were ingested far less readily than granules. Why this is the case is currently unclear. Perhaps their elongated shape make fibers harder to capture, handle, and ingest, or, when consumed, fibers are more prone to causing physical damage owing to their sharp edges (Supporting Information, Figure S1); conversely, granules are relatively spherical in shape, and are likely handled similarly to naturally occurring particulates (e.g., pumice, wood, black carbon, and silt) to which copepods are well adapted. The risks microplastic fibers pose to biota is relatively underexplored, however exposure studies have identified that in the freshwater zooplankton Daphnia magna and Gammarus fossasrum ingestion of synthetic fibers resulted in early mortality 50 and impaired feeding 51 respectively.
Lipids. In juvenile C. f inmarchicus (CV), approximately 40% of energy derived from their food goes toward the buildup of their lipid store. 49 On the basis of the observed reduction in feeding in juvenile C. f inmarchicus exposed to microplastic fibers and the shift to smaller, less nutritious algae, it was anticipated that the lipid mass of these copepods would be negatively affected; furthermore, we surmised that a shift in feeding may result in an altered lipid profile. Yet, there was no significant difference in the total lipid mass of juvenile, female, or male copepods at the end of the exposure period, nor were the lipid profiles of the juvenile copepods significantly altered. Given that a nonlimiting supply of food was provided to the copepods, it is plausible that even with a 40% reduction in ingested biomass that the juvenile C. f inmarchicus still consumed sufficient energy to continue laying down their lipid reserves. Certainly, high latitude zooplankton can display a range of strategies to survive periods of low food availability, 52 and C. finmarchicus exhibit far greater starvation tolerance (>21 days) than temperate species. 39 We did observe that lipid accumulation was stymied in preadult copepods exposed to nylon granules. This was surprising given that nylon granules caused no impact on feeding rate. This intriguing result might be explained by the

Environmental Science & Technology
Article substantially higher ingestion rate for granules, as compared with fibers: high microplastic loads in the intestinal tract could limit assimilation efficiencies, as observed in the freshwater amphipod G. fossarum; 53 alternatively, higher microplastic loads could lead to greater quantities of toxic additives or monomers permeating from the microplastic into tissues. 51 Lipid mass is directly related to the depth at which copepods can successfully descend during diapause; smaller lipid reserves would result in overwintering at shallower depths leaving these copepods more prone to predation. 54 There are also repercussions for the wider marine food web, as a reduced lipid content would make these copepods less nutritionally valuable as a food source for higher trophic organisms including commercially important fish species and megafauna. 36 Moulting. Our study further identified that copepods exposed to nylon microplastics moulted significantly earlier than copepods in the control treatment. Juvenile C. f inmarchicus have a flexible life history, where they can either enter diapause or moult into their adult life stage. Tarrant et al. 40 notes, "the factors that regulate this developmental plasticity are poorly understood", although lipid profiles, temperature, light, food availability, and endogenous clocks have all been mooted as contributing factors in diapause. 55 Cultured C. f inmarchicus do not initiate diapause, instead undergoing morphological changes, including gonad maturation, tooth formation, and apolysis (separation of the cuticle from the epidermis), prior to their terminal moult. 56 Reduced feeding and stymied lipid accumulation may both have contributed to earlier moulting; however endocrine disruption might also have played a role. A transcriptomic evaluation of juvenile C. f inmarchicus (CV) has identified an array of genes linked to moulting, activated by an ecdysteroid hormone signaling cascade. 40 A range of endocrine disrupting compounds have been shown to interfere with ecdysteroid pathways and affect moulting in crustacea; 57 for example, estrogenic compounds have been shown to inhibit or delay moulting in the copepod Acartia tonsa, 58 while the pesticide emamectin benzoate has been demonstrated to cause premature moulting in American lobster, Homarus americanus. 59 Chemical analysis revealed the nylon microplastics used in these exposures contain compounds that may cause toxicity or endocrine disruptionalthough it should be noted this analysis does not tell us which compounds, nor how much, could be expected to leach from the nylon either in seawater or the intestinal tract of a copepod. For example, the UVstabilizer benzophenone, identified in the nylon fibers, has been shown to increase DNA methylation and significantly reduce egg hatching success in the marine copepod Gladioferens pectinatus, 60 and act as an oestradiol agonist in rats 61 and fish. 62,63 It is crucial to recognize that plastics are not an inert material, but a complex mixture of polymers containing a wide spectrum of compounds that have the potential to leach out. 64 Given the capacity for these compounds to cause endocrine disruption or toxicity, it is crucial that chemical profiling of microplastics used in toxicity testing becomes more commonplace.
Environmental Relevance. In this exposure study we demonstrate that microplastics have the capacity to reduce feeding, stymie lipid accumulation, and trigger premature moulting in a boreal copepod. It should be noted that microplastic concentrations used in our exposure studies exceed those currently observed in the marine environment although we would also highlight there is very little environmental data relating to concentrations of particles 10−30 μm in size owing to the technical challenges of sampling, extracting and identifying plastic particles of this size and where data are available, it suggests the smaller the microplastics the higher the concentration becomes. 65−68 While it is important the field of microplastics research shifts toward better understanding the risks environmentally relevant concentrations of microplastic pose to marine life, at this stage it remains essential to build a clearer picture of the modes of action by which microplastics can cause harm, identify relevant end points, and gauge the sensitivity of different life-stages and species. 69 Such knowledge is key in establishing probable and no-effect thresholds for risk assessment. In this study, the use of preadult copepods highlights that microplastics can affect moulting, which will inform future experimental work. As our results demonstrate, the shape and chemical profile of a microplastic can influence bioavailability and toxicity, and we would therefore promote the call for future studies to better incorporate a greater diversity of environmentally relevant microplastics.

* S Supporting Information
The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.est.9b01853.
Images of the microalgae, microplastics, and copepods; chemical data for the nylon fibers and granules; data relating to the size and shape of microalgae; and fatty acid and alcohol data for copepods in control, fiber, and granule treatments (PDF)

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Article