Metal-Dependent Cytotoxic and Kinesin Spindle Protein Inhibitory Activity of Ru, Os, Rh, and Ir Half-Sandwich Complexes of Ispinesib-Derived Ligands

Ispinesib is a potent inhibitor of kinesin spindle protein (KSP), which has been identified as a promising target for antimitotic anticancer drugs. Herein, we report the synthesis of half-sandwich complexes of Ru, Os, Rh, and Ir bearing the ispinesib-derived N,N-bidentate ligands (R)- and (S)-2-(1-amino-2-methylpropyl)-3-benzyl-7-chloroquinazolin-4(3H)-one and studies on their chemical and biological properties. Using the enantiomerically pure (R)- and (S)-forms of the ligand, depending on the organometallic moiety, either the SM,R or RM,S diastereomers, respectively, were observed in the molecular structures of the Ru- and Os(cym) (cym = η6-p-cymene) compounds, whereas the RM,R or SM,S diastereomers were found for the Rh- and Ir(Cp*) (Cp* = η5-pentamethylcyclopentadienyl) derivatives. However, density functional theory (DFT) calculations suggest that the energy difference between the diastereomers is very small, and therefore a mixture of both will be present in solution. The organometallics exhibited varying antiproliferative activity in a series of human cancer cell lines, with the complexes featuring the (R)-enantiomer of the ligand being more potent than the (S)-configured counterparts. Notably, the Rh and Ir complexes demonstrated high KSP inhibitory activity, even at 1 nM concentration, which was independent of the chirality of the ligand, whereas the Ru and especially the Os derivatives were much less active.


■ INTRODUCTION
Undoubtedly, there has been considerable progress in contemporary cancer medicine, but many tumor types are still difficult to treat. Commonly used anticancer drugs, such as tubulin binding agents, taxanes (e.g., paclitaxel, docetaxel), 1−5 and Vinca alkaloids (vincristine and vinblastine) 6 exhibit numerous side effects. 7 Therefore, it is crucial to develop new compounds exhibiting fewer or, ideally, no side effects. This can be achieved through applying a variety of strategies, such as structural modification of tubulin-binding agents or design of new low molecular weight inhibitors of proteins involved in cell division. A vast number of antimitotic agents have been synthesized 8−12 or isolated from natural sources, 13 and their biological properties have been extensively evaluated. To date, however, only a few semisynthetic antimitotic agents are used for cancer treatment, while others are currently in clinical trials. 14 −16 Mitosis, which is a strictly regulated multistep process, can be disturbed at different stages by selective inhibitors of cell cycle regulators (e.g., polo-like kinase 1, PLK1) 17 or spindle checkpoint proteins (e.g., Aurora A, Aurora B, CENP-E, KSP). 18−20 The therapeutic potential of inhibitors targeting aurora A (e.g., PHA-739358, MLN8237), 18 aurora B (e.g., PF-03814735, AZD1152), 18 and PLK1 (e.g., BI2536, ON 01910) 21,22 is relatively well established. Kinesins (e.g., KSP and CENPE) have been explored as molecular targets in cancer therapy since 1999, when the first low molecular weight inhibitor of a kinesin spindle protein (KSP, also known as kinesin-5 or Eg5), monastrol, was discovered. 23 KSP is a member of the motor proteins, playing a crucial role in spindle pole separation. It is highly active in dividing cells while almost undetectable in nondividing cells. As its inhibition results in mitotic catastrophe and leads to apoptosis, KSP is a promising molecular target for modern antimitotic anticancer drugs. A large number of structurally diverse molecules have been identified as KSP inhibitors, and some have entered clinical trials. 24−26 The most active KSP inhibitors include MK-0731, 24 ARRY-649, 27,28 ispinesib, 28,29 and its chromone analogue SB-743921. 29 The tremendous progress in bioorganometallic chemistry has resulted in a large number of new compounds of unusual biological activity, for example, by combining organometallic compounds with biologically active molecules. 30,31 Such conjugates are often more potent antiproliferative agents than their parent compounds and sometimes exhibit additional biological properties. To date, the most intensively studied derivatives are sandwich 31 (mainly ferrocene and ruthenocene) and half-sandwich derivatives of Ru, 32,33 Os, 33,34 Rh, 35 and Ir. 35 We and others studied the influence of organometallic moieties on the biological activity of antimitotic tubulinbinding agents such as taxanes, 36,37 colchicine, 38−41 plinabulin, 11 or podophyllotoxin. 42,43 We found that replacing the Nbenzoyl moiety of paclitaxel with a ferrocenoyl moiety significantly increases its ability to induce polymerization of tubulin and the antiproliferative potency of such conjugates is higher than that of the parental taxane. 36,37,44 In the case of plinabulin, we found that replacing the phenyl moiety with ferrocene leads to compounds able to inhibit clinically relevant multidrug resistance transporters ABCB1 and ABCG2. 11 The observed unexpected positive impact of an organometallic moiety on the biological activity of antimitotic agents encouraged us to study organometallic conjugates of the potent KSP inhibitor ispinesib 1. Herein, we report new halfsandwich complexes of Ru, Os, Rh, and Ir bearing 2-(1-amino-2-methylpropyl)-3-benzyl-7-chloroquinazolin-4(3H)-one 2 found in ispinesib as an N,N-bidentate ligand showing inhibitory activity of KSP and potent cytotoxic activity.
General Procedure for the Synthesis of Ru and Os Complexes 3a−4b. (S)-2 or (R)-2 was dissolved in 10 mL of methanol by sonication for about 5 min. [(cym)MCl 2 ] 2 (M = Ru and Os, cym = η 6 -p-cymene) was added in one portion to this solution, and the resulting reaction mixture was stirred overnight at 50°C. Upon the addition of NH 4 PF 6 , stirring was continued for another 1−2 h, after which the solvent was removed under reduced pressure. The residue was dissolved in 20 mL of dry DCM, and the solution was gravity filtered to remove inorganic salts. The filtrate was reduced in volume to about 5 mL, and about 30 mL hexane was added. The resulting solution was kept at 4°C for 1−2 days to obtain a yellow crystalline solid, which was filtered and dried to isolate pure products.
[(cym)Ru{(R)-2)}Cl][PF 6 ] (3a). Compound 3 was prepared in 83% yield (497 mg) by following the general procedure using 269 mg (0.790 mmol) of (R)-2, 237 mg (0.287 mmol) of [(cym)RuCl 2 ] 2 , and 1.070 g (6.564 mmol) of NH 4  General Procedure for the Synthesis of Rh and Ir Complexes 5a−6b. A solution of 1 equiv of ligand (R)-2 or (S)-2 in an appropriate amount of methanol was added to a slurry of 0.5 equiv of [(Cp*)MCl 2 ] 2 (M = Rh or Ir, Cp* = η 5 -pentamethylcyclopentadienyl) in 5 mL of methanol, and the resulting solution was stirred at RT under an argon atmosphere for 1 h. The solvent was evaporated, and the residue was dissolved in an appropriate amount of water. A saturated solution of KPF 6 (3 mL) in water was added to this solution. The product was extracted with dichloromethane (3 × 10 mL). The organic fractions were combined and dried over magnesium sulfate, and the solution was evaporated to dryness. The crude product was dissolved in a minimal amount of hot methanol, and the resulting solution was kept in the freezer for 48 h at −30°C. Crystals of pure products were collected by filtration, washed with cold methanol, and dried.

■ RESULTS AND DISCUSSION
The half-sandwich complexes 3a−6b were synthesized in one step reactions starting from optically pure ligands (R)-2 for 3a−6a or (S)-2 for 3b−6b and the corresponding dimetallic precursors [(cym)MCl 2 ] 2 (M = Ru for 3, M = Os for 4) or [(Cp*)MCl 2 ] 2 (M = Rh for 5, M = Ir for 6). All complexes were prepared in the reaction of enantiomerically pure 2 with 0.5 equiv of the corresponding dimetallic precursors in methanol at RT or 50°C and were isolated as hexafluorophosphate salts in good to excellent yields (Chart 1). The structures of these complexes were established by NMR spectroscopy, MS, and X-ray crystallography. The complexation of ligands (R)-2 and (S)-2 to the metal centers results in significant changes in the NMR spectra. For example, in the 1 H NMR spectra, the H-8 signal resonated at 7.68 ppm in 2, while in case of the complexes the proton was detected at 8. 51, 8.37, 8.26, and 8.20 ppm for 3−6, respectively. The diastereotopic protons of the NH 2 group coordinated to the metal centers were observed as wellseparated multiplets at 6.41 and 3.16 ppm for 3, at 7.19 and 4.12 ppm for 4, at 4.55 and 3.46 ppm for 5, and at 5.44 and 4.20 ppm for 6.
It might be expected that a mixture of diastereoisomers would be formed in the complexation reactions of ligands (R)and (S)-2; however, only one main set of peaks for each complex 3a−6b was detected by NMR spectroscopy together with small amounts of a second species, possibly due to ligand exchange in solution. The configuration of the products in CDCl 3 or CD 2 Cl 2 solutions was determined by 2D NOE experiments. For Rh and Ir complexes 5a−6b, key NOE contacts between Cp* protons and H-8, NH 2 , and CH 3 protons of the aminoisobutyl chain were detected, while for Ru and Os complexes, contacts between cym aromatic protons and H-8 and between aminoisobutyl chain and phenyl, amine, and CH 2 −benzyl protons were found ( Figure 1 for 3b and 5a).
To investigate whether the complexes exist in solution as a mixture of diastereoisomers, we recorded NMR spectra at a low temperature in CD 2 Cl 2 . As shown in Figure 2 for Rh complex 5b, decreasing the temperature to 253 K results in the coalescence of some peaks, e.g., those assigned to the PhCH 2 and i Pr groups. Lowering the temperature further to 203− 213 K results in the formation of well-separated peaks due to the low exchange rate. The process was found to be reversible, and the spectra recorded at RT were identical to the initially recorded ones. Similar behavior was observed for the other complexes (Figures S1−S3).
Single crystals suitable for X-ray diffraction analysis were obtained by slow diffusion of n-hexane into solutions of the Ru and Os complexes in DCM, while in the case of the Rh and Ir complexes, crystals were grown by slowly cooling saturated methanolic solutions, which were stored at −30°C for 48 h. The Ru and Os complexes 3b and 4a bearing the (S)-2 and (R)-2 ligands, respectively, crystallized in the P2 1 2 1 2 1 and P2 1 space groups. Compounds 5a and 6a and compounds 5b and 6b crystallized in the enantiomorphic pairs of P6 1 (a) and P6 5 (b) space groups, respectively. The latter compounds are isostructural and form analogous crystal lattices in the solid state.
All of these compounds displayed pseudo-octahedral geometry at the metal center with the piano-stool configuration. As expected, ligands (R)-2 and (S)-2 acted as N,Nchelators, forming five-membered rings with the metal ions by coordination through the NH 2 nitrogen and N−1 of the quinazoline moiety (Figures 3 and S4). In all cases, single diastereomers were isolated with the cym complexes 3b and 4a showing R Ru ,S and S Os ,R configurations, while in case of the Cp* compounds 5a, 5b, 6a, and 6b, they were found to be R M ,R or S M ,S. In the cym complexes, the arene ligand is in anticonfiguration to the isopropyl group of the quinazoline ligand and syn to the benzyl group of the quinazoline ligand, while it is vice versa for the Rh and Ir complexes with the Cp* ligands. In neither of the structures were H-bonding or πstacking observed. The bond lengths around the metal center were for all complexes in similar ranges ( Table 1). The Rh derivatives had slightly larger bond lengths from the Rh center Inorganic Chemistry pubs.acs.org/IC Article to the endocyclic nitrogen atom than found for Ir and especially Ru and Os.
In order to explore the foundation for the formation of crystals from different diastereomers dependent on the πbound ligand at the metal center, density functional theory (DFT) calculations were conducted with Gaussian 09W. Calculation of the energetic differences in the ground state between the Ru and Rh complexes (3a, 3b, 5a, and 5b, Table  S2) and dependent on the enantiomer of ligand 2 revealed that the energies were very similar for the stereoisomers of the different metal centers (Ru, ∼0.3 kcal/mol; Rh, ∼0.4 kcal/ mol). This suggests that the stereochemistry does not determine a preferential formation of one or the other stereoisomer. It is more likely that the isolation of pure diastereomers is a result of the more facile crystallization of one of them.
NMR spectroscopic investigations in different solvents showed that the prepared complexes have differing stability in solution. In contrast to the Rh and Ir derivatives, the Ru and Os complexes are stable in aprotic solvents such as CDCl 3 or CD 2 Cl 2 . The 1 H and 13 C{ 1 H} NMR spectra of the Ru and Os complexes showed one set of signals, while in the case of Rh and Ir compounds, additional sets of signals were present immediately after dissolving of the samples in CD 2 Cl 2 ( Figures  S22−S37), with the large majority of the dissolved complexes remaining intact (>95% for Rh and >85% for Ir).
Metal−chlorido ligand bonds are often labile, and ligand exchange can occur in H 2 O, methanol, or DMSO solutions. 49−51 Since usually the test compounds are dissolved in DMSO for biological studies, we investigated the stability of such solutions by 1 H NMR and UV−vis spectroscopy, as well as ESI-mass spectrometry. The 1 H NMR spectra showed that all complexes undergo changes in DMSO-d 6 solutions, resulting in the formation of a new set of signals ( Figures  S5−S8); however, the resulting solutions were stable in the dark for days. We observed differing kinetics in the chloride/ solvent exchange of the studied complexes for the Ru/Os and Rh/Ir couples. The Rh complexes undergo an extremely fast    S12) and UV−vis spectra ( Figure S14) confirmed that the complexes are sufficiently stable in DMSO solution in the dark, and only minor changes in the UV−vis spectra were observed, which may be assigned to chlorido/ DMSO ligand exchange.
In methanol solution, the Ir and Ru complexes underwent very fast cleavage of the M−Cl bonds (ca. 31%), occurring just after preparation of the solution, while their Os and especially Rh counterparts were more resistant toward such reactions with 22% of the Os and 12% of the Rh complex undergoing chlorido ligand exchange. This process can be suppressed by the addition of lithium chloride (as saturated solution in CD 3 OD) and in the presence of a high concentration of chloride ions; this reaction is almost completely reversible (for 6b see Figure 4 and Figure S13).
Dulbecco's Modified Eagle's Medium (DMEM) consists of numerous organic compounds which may act as ligands for organometallics. Therefore, we studied the solution behavior of the synthesized compounds in DMEM using UV−vis spectroscopy, mimicking cell culture conditions by adding the DMSO solutions of the complexes (100 μM) while keeping the DMSO concentration as low as 0.5%. The UV−vis spectra indicated that all compounds reacted within minutes with DMEM components (Figures S15−S18 and Table S3). On the basis of the DMEM composition and previously reported data for Ru complexes, 52−54 we selected L-cystine (CysCys) and Lhistidine (His) for further studies. Both substances are prominent components of DMEM (0.2 mM). UV−vis spectra and LC-MS analysis of freshly prepared solutions of the organometallic complexes (100 μM) and the amino acids (200 μM) revealed that the Ir and Rh complexes afforded a mixture of organometallic species when incubated with His. The UV−vis spectra of mixtures of 5a and 6a with His showed the quick formation of new species (for 5a see Figure S19, for 6a see Figure S20), as was observed for incubation mixtures of 5a and 6a in DMEM (Figures S17 and S18, respectively). The LC-MS data collected for a freshly prepared mixture of Ir complex 6a with His ( Figure S21 Antiproliferative Activity. A high level of inhibition of KSP activity may result in significant antiproliferative effects in cancer cells. 55,56 Therefore, the cytotoxicity of (R)-2 and (S)-2 and their organometallic complexes 3a−6a and 3b−6b toward human colorectal carcinoma (HCT116), hepatocellular carcinoma (HepG2), large cell lung cancer (NCI-H460), colorectal adenocarcinoma (SW480 and SW620), and cervical squamous cell carcinoma (SiHa) cells was studied ( Table 2). The cell lines used exhibited variable sensitivity to the parental compounds, (R)-ispinesib and (S)-ispinesib ((R)-and (S)-1, respectively). (R)-1 exhibited high cytotoxicity toward the majority of cells studied with IC 50 values <8 nM for most of the studied cancer cell lines with the exception for HepG2, for which the IC 50 value was 0.53 ± 0.01 μM. The corresponding (S)-enantiomer (S)-1 was characterized by significantly lower cytotoxicity with IC 50 values in the high nanomolar or even Metal complexation did not significantly alter the activity of (R)-2 derivatives in the cell lines tested, except for moderately elevated effects of the Ir complex 6a. In contrast, although the antiproliferative potential of (S)-2 is significantly lower than that of (R)-2, metal complexation elevated its activity. The Ru and Os complexes 3b and 4b of (S)-2 exerted potent biological effects demonstrating between 2-fold (3b in SiHa cells) and 9-fold (4b in HCT116) higher activity than the parent organic ligand, showing a clear impact of metal coordination. Considering the individual cell line vulnerability, HCT116 proved to be the most sensitive cell line to the compounds investigated, including ispinesib. Additionally, all the complexes of (R)-2 were similarly potent in this cell line as the ligand itself.
KSP Inhibition. Ispinesib is a well established KSP inhibitor with IC 50 values reported to be in the range of 0.5−4.6 nM. 57 The ability of the synthesized compounds to inhibit KSP activity was studied by the ATP hydrolysis assay. We found that the Rh/Ir complexes 5a, 5b, 6a, and 6b exhibited higher activity in comparison to the ispinesib-derived ligands (R)-2 and (S)-2 ( Figure 5), even at concentrations as low as 1 nM. In contrast, the Ru and Os complexes are significantly less active. Comparison of the Rh and Ir complexes bearing the (S)-2 or (R)-2 ligands revealed that the observed KSP inhibitory activity is virtually independent of their conformation. In contrast, the Ru and Os (R)-2-type complexes were found to be significantly stronger KSP inhibitors than analogous (S)-2 complexes, with the former leading to a maximal inhibition of approximately 40% at 100 nM concentration. These results are especially interesting in view of the fact that ruthenium complexes of another KSP inhibitor, monastrol, were shown to exhibit lower KSP inhibitory activity than the parent compound. 58,59 Docking Study. In order to elucidate contrasting structural features of relevance to protein interaction for the organometallic compounds, molecular modeling was used to investigate the possible binding mode for the ligands (R)and (S)-2 and their half-sandwich complexes 3a−6b as well as the respective diastereomers 3a′, 3b′, 5a′, and 5b′ to KSP. While the complexes may undergo ligand exchange in aqueous solution to form aqua complexes, or in the presence of proteins to form covalent bonds, for the reason of comparability, we decided to dock the chlorido complexes. The KSP cocrystal structure with ispinesib derived from Homo sapiens was obtained from the Protein Data Bank (PDB ID: 4AP0; resolution 2.59 Å). 60 Co-crystallized ispinesib was removed and redocked to the allosteric site with excellent docking overlay (RMSD = 0.356 Å for GoldScore; Table S4 and Figure  S25A). Ispinesib is buried in the allosteric site and displays numerous interactions with residues of the inhibitor-binding pocket. 61 Both the cocrystallized ligand and its redocked configuration showed that the benzyl moiety of the ligand is 110 ± 77* 39* (too wide) 14 ± 2 2 9 ± 3 4 0 ± 2 2 6 ± 1 a (*) Over range; (too wide), the curve drops down rapidly so no SD can be calculated. Inorganic Chemistry pubs.acs.org/IC Article buried deeply in the hydrophobic part of the pocket, where it stacks with the Pro137 ring and makes an edge-to-face interaction with the side chain of Trp127. Moreover, it is found to be in lipophilic contact with the side chains of Tyr211, Ala218, Ala133, Glu118, Arg119, Leu160, and Leu214 ( Figure S25B). The similarities in the docked configurations to the one described in the literature 23,61−63 and a low RMSD value suggest the reliability and reproducibility of the docking protocol.
The spatial orientations and interactions (Tables 3 and S5) found for (R)-and (S)-2 and their half-sandwich complexes 3a−6b as well as the respective diastereomers 3a′, 3b′, 5a′, and 5b′ show that the compounds may bind to the same site as previously reported, 60−64 which is suggestive to be related to their anticancer activity. Both (R)-and (S)-2 show similar interactions with the protein with the main difference being a hydrogen bond of the latter to Tyr211 and a slightly higher GoldScore for the (R) enantiomer.
For the organometallic compounds, in general, the S M ,R and R M ,R isomers showed higher predicted scores than the isomers derived from (S)-2 with the exception of the Rh complex pair 5a′/5b′ (Table 3). While the isostructural compounds 3a and 4a are found in virtually identical positions in the structure, their diastereomers 3b and 4b behaved very differently, with the Os complex giving a lower docking score than the Ru analogue and interaction with a limited number of amino acids (Table S5). However, these differences have a big impact on the cytotoxic activity and the KSP inhibition with (R)-2 based 3a and 4a being more potent than 3b and 4b in both assays. Surprisingly, the observations were very different for the Rh and Ir complexes. While the isomers 5a/5b and 6a/6b follow a similar pattern in the cytotoxicity assay, they are equally potent in inhibiting KSP independent of the configuration of the ligand or the metal center. This trend is also reflected in the GoldScores found for these compounds, which are very similar but significantly lower than those found for the Ru and Os complexes of (R)-2. This suggests that for the latter classes of compounds KSP may not be the target, but their cytotoxic potential is exhibited through other means.
The docked configurations of Rh complexes 5a and 5b into the binding site are shown in Figure 6. For 5a, the Cp* moiety Table 3. GoldScores for the Docking Scores of (R)-and (S)-2 and Their Half-Sandwich Complexes 3a−6b As Well As the Respective Diastereomers 3a′, 3b′, 5a′, and 5b′, Dependent on the Chiral Configuration at the Metal Center and Ligand Inorganic Chemistry pubs.acs.org/IC Article is embedded deeply in the hydrophobic pocket formed by Leu214, Ala133, and Trp127 ( Figure 6A). The quinazolinyl moiety interacts with parts of the side chain of Arg119, and the chlorine substituent of the quinazolinyl moiety sits in another mostly hydrophobic pocket formed by the side chain of Ala133 and Pro137. The benzyl moiety of the ligand is not inserted into the active site pocket and is more exposed to the aqueous phase, especially when compared to 3a and 4a, which gave much higher docking scores in a similar range to ispinesib (Table S4). However, the isopropyl group is only partly buried and interacting with parts of the side chains of Tyr211 and Ala218 ( Figure 6A). Upon docking of the 5b isomer, the ring systems are "flipped" relative to 5a ( Figure 6B), and in this case the Cp* ligand is more exposed to the aqueous phase. The carbonyl group of the quinazolinyl moiety exhibited hydrogen bonding with the side chain of Arg119. The benzyl group sits deep in the hydrophobic pocket and forms an edge-to-face stacking interaction with the side chains of Ala133 and Arg119. The isopropyl group is embedded deep and orientated toward the binding pocket of ispesinib. In addition to the diastereomers characterized by X-ray diffraction analysis, we docked diastereomeric structures to 3a, 3b, 5a, and 5b, i.e., 3a′, 3b′, 5a′, and 5b′ (Tables 3 and S5), to investigate the impact of the π-bound ligand and of chirality on the docking behavior. Comparison of the GoldScores of isostructural 3a/4a and 5a′ shows that the Ru-and Os(cym) complexes resulted in much higher scores than their Rh(Cp*) counterpart. The effect was found to be less pronounced for the complexes featuring (S)-2, but the trend is the same. In both cases, the structures of the complexes were orientated very differently for the docking result, as they were when comparing 5a/6a with 3a′ and 5b/6b with 3b′.
Overall, there are significant differences in the docking results for the different isomers of the organometallics both in terms of orientation but also in terms of their interactions with the protein, which may have an impact on their modes of action. This is also supported by the biological data collected for the compound classes, under the limitations of docking as outlined above regarding the possibility of chlorido/aqua ligand exchange and/or coordination to donor atoms of the protein. The obtained results correlate with the experimental inhibitor activity of KSP only in discriminating of the stereochemistry of ligand 2 but do not correlate with distinguishing of the organometallic moieties.
ROS Generation. It is often postulated that organometallic complexes induce oxidative stress in cancer cells. 31 Therefore, we studied the generation of reactive oxygen species (ROS) induced by complexes 3a−6b in SW620 and HepG2 cells, differing in sensitivity toward the investigated compounds, using the dihydrorhodamine 123 (DHR123) oxidation assay ( Figure 7). Surprisingly, the overall pattern of DHR123 oxidation was similar between these two cell lines, indicating that the redox balance disturbance is independent of the antiproliferative potential of the compounds studied. It must be noted, however, that both osmium complexes consistently decreased the oxidation rate of DHR123 compared to the control. DHR123 is considered a nonspecific reactive oxygen/ nitrogen species indicator, and therefore these results suggest that either some intracellular source of ROS is inhibited by the investigated compounds or the compounds themselves exhibit some antioxidant activity. Such activity was demonstrated, for example, for Ni(II) quinazoline complexes and Mn-porphyrins, acting as low molecular weight superoxide dismutase mimics. 65,66 To test these hypotheses, we performed a robust ABTS [2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)] radical cation reduction assay. 67 We were able to demonstrate that both Os (4a and 4b) and to a lesser extent also Ru (3a and 3b) complexes exhibited antioxidant properties ( Figure 8). It can be therefore inferred that the osmium complexes add to the antioxidant pool of the cell and thus reduce the DHR123 oxidation rate.
Cellular Accumulation. The antiproliferative potential of anticancer agents is, at least to some extent, dependent on their cellular accumulation. Therefore, we aimed to determine the amount of the respective metal accumulated in SW620 cancer cells by ICP-MS following exposure to the synthesized complexes. The choice of the cell line was dictated by the facts that (i) it responds well to ispinesib ( Table 2) and (ii) it does not express any of the major multidrug resistance proteins that could alter the influx kinetics of the investigated compounds. 68 However, this experimental approach based on the sole metal measurement does not allow for discrimination between the metal complex accumulated inside the cell or bound to the cell surface. In this case, there is no clear correlation between cytotoxic/cytostatic effects and the determined metal concentration. For all compounds, the amount of metal detected was highest after 1 h and declined over time (Figure 9). The Os complexes reached a significantly Inorganic Chemistry pubs.acs.org/IC Article higher concentration than the corresponding Ru complexes (up to ca. 16 nmol Os/mg cellular protein), while the more cytotoxic 5b and 6b complexes were detected in concentrations up to 1.4 and 3.4 nmol of metal/mg protein, respectively. Interestingly, for complexes bearing (S)-2 as the ligand, slightly higher values were found compared to the more active derivatives of (R)-2. As mentioned before, Ir and, especially, Rh complexes tend to release (R)-and (S)-2 when incubated with histidine and/or cystine, which presumably may also occur in a culture medium. Metal species may enter the cells by the ligand conveyor mechanism similar to that postulated for phosphine gold complexes in the case of auranofin 69 but may be then readily exported from the cytoplasm, presumably with glutathione, as was demonstrated for cisplatin. 70 This would explain the observations in terms of detected low metal content in cell uptake studies, high antiproliferative activity, and excellent KSP inhibitory potency.

■ CONCLUSIONS
Two series of organoruthenium, -osmium, -rhodium, and -iridium complexes with the ligands (R)-and (S)-2-(1-amino-2-methylpropyl)-3-benzyl-7-chloroquinazolin-4(3H)-one were synthesized. The series differed in their antiproliferative potentials, with the complexes of the (R)-configured ligands exerting significantly stronger biological effects with IC 50 values in the high nanomolar range for the most susceptible cell line tested as compared to low to medium micromolar activity for the corresponding (S)-analogues. Interestingly, the effect of the metal center on the antiproliferative potential of the investigated compounds was rather moderate. On the other hand, introduction of a metal center significantly affected the anti-/prooxidative properties, cellular accumulation, and inhibitory effects on KSP. The Os and Ru complexes inhibited ROS formation, avidly accumulated in cells, and exerted low to moderate effects on KSP activity. On the other hand, the Rh and Ir complexes were potent KSP inhibitors, despite the low stability of the Rh complexes which warrants further investigation. The level of their cellular accumulation/binding was an order of magnitude lower, and their effects on intracellular reactive oxygen species level were negligible. These observations may be at least partially explained by differences in the susceptibility of the metal complexes for ligand exchange reaction.
■ ASSOCIATED CONTENT