A Molecular Hybridization Approach for the Design of Potent, Highly Selective, and Brain-Penetrant N-Myristoyltransferase Inhibitors

Crystallography has guided the hybridization of two series of Trypanosoma bruceiN-myristoyltransferase (NMT) inhibitors, leading to a novel highly selective series. The effect of combining the selectivity enhancing elements from two pharmacophores is shown to be additive and has led to compounds that have greater than 1000-fold selectivity for TbNMT vs HsNMT. Further optimization of the hybrid series has identified compounds with significant trypanocidal activity capable of crossing the blood–brain barrier. By using CF-1 mdr1a deficient mice, we were able to demonstrate full cures in vivo in a mouse model of stage 2 African sleeping sickness. This and previous work provides very strong validation for NMT as a drug target for human African trypanosomiasis in both the peripheral and central nervous system stages of disease.


■ INTRODUCTION
Human African trypanosomiasis (HAT), or sleeping sickness, is caused by two different subspecies of the protozoan parasite Trypanosoma brucei: T.b. gambiense and T.b. rhodensiense. The disease, which is fatal unless treated, is transmitted by the bite of an infected tsetse fly. It has two stages: an initial peripheral infection during which the parasites are found in the bloodstream, giving rise to nonspecific symptoms, and a second stage when the parasites enter the central nervous system, causing the classic symptoms of HAT, eventually leading to coma and death. Currently, there are five treatments available, although none of them are satisfactory due to toxicity, treatment failures, or the requirement for parenteral administration, which is inappropriate in a rural African setting. 1 N-Myristoyltransferase (NMT) represents a potential drug target for HAT because in T. brucei RNAi knockdown of NMT has been shown to cause cidality in cell culture and to abrogate infectivity in several animal models. 2,3 We have also reported the successful chemical validation of TbNMT using the prototype NMT inhibitor DDD85646 (2) derived from pyrazole sulfonamide screening hit DDD64558 (1) (Scheme 1), which is highly effective at curing mouse models of hemolymphatic (peripheral) infection. 4,5 Unfortunately, we were not able to obtain conclusive evidence for TbNMT validation in stage 2 disease as the blood−brain barrier penetrant compounds we developed did not have a sufficient therapeutic window. 6 Several research groups have also interrogated NMT as a potential target for the treatment of leishmaniasis, 7,8 malaria, 9 fungal infections, and cancer. 10−21 In most/all of these inhibitors, there is a basic center in the compound that maintains a key interaction with the C-terminal carboxylic acid, found in the peptide binding site of NMT.
NMT catalyzes the cotranslational transfer of myristate from myristoyl-CoA to the N-terminal glycine of ∼60 proteins; a modification that is implicated in localization and/or activation of the substrate−proteins. 22 The enzyme operates via a Bi−Bi mechanism in which it first binds myristoyl-CoA, causing a conformational rearrangement that subsequently reveals the peptide binding site. 2 There is a significant deficit in our understanding of the identity of downstream targets of NMT in T. brucei; however, the ribosylation factors Arf and Arl have been shown to be NMT substrates important for survival of the parasite in cell culture. 23 Attempts to crystallize TbNMT have been unsuccessful to date. However, we were able to obtain a structure for Leishmania major NMT (LmNMT), which we have used as a surrogate model for TbNMT ( Figure 1A). LmNMT is the closest homologue of TbNMT for which a crystal structure has been elucidated: the primary sequence id was 74% identical overall and 94% identical within the ligand binding cavities. Structural analysis was carried out on selected pyrazole sulfonamide ligands and has been previously described in detail. 4,5 The ligands occupy the substrate binding groove of LmNMT with the pyrazole occupying a pocket that binds residues 4 and 5 of the peptide substrate. The pyrazole nitrogen lone pair forms a key hydrogen bond with the side chain hydroxyl group of Ser330 ( Figure 1B). The pyrazole Nmethyl group interacts with the side chains of Val81 and the aromatic ring of Phe90, and consequently, larger substituents than methyl were not tolerated ( Figure 1B). There was a degree of flexibility in the position and orientation of the sulfonamide group. For most ligands within the series, the Scheme 1. Pyrazole Sulfonamide NMT Inhibitors ("S" = HsNMT IC 50 /T. brucei EC 50 ): DDD64558 (1), 5 DDD85646 (2), 5

Journal of Medicinal Chemistry
Article sulfonamide moiety hydrogen bonded through a highly coordinated water molecule to the side chain of His219 and the backbone amides of Asp396 and Gly397. In other ligands (described elsewhere 5 ), the water-bridged interaction with His219 was replaced by a direct hydrogen bond between the sulfonamide oxygen and the side chain of Asn376. The aryl rings of DDD85646 (2) did not form directional interactions with the protein, though they stacked above the side chain of Tyr217, which lines the floor of the peptide binding site. Hydrophobic packing was increased further by the two chlorine atoms of compound 2, explaining their beneficial effect on potency.
Significant gains in potency were obtained by appending a basic functionality, which formed an ionic interaction with the carboxylate of the C-terminal residue (Leu421) and a series of water-mediated hydrogen bonds. In the case of 2, this was obtained with a constrained amine, but it was subsequently shown that similar gains in potency could be obtained with a more flexible analogue such as DDD100097 (3) (Scheme 1). 6 Lead optimization of the pyrazole sulfonamides subsequently led to the identification of potently trypanocidal brainpenetrant inhibitors, which were evaluated for efficacy in a stage 2 mouse model of HAT (e.g., DDD100097 (3)). 6 Unfortunately, a fully curative dose regimen was not achieved due to dose-limiting toxicity. This toxicity is likely to be associated with the simultaneous blockade of host NMT (Mouse NMT is 98% identical to Human NMT (HsNMT)), although it is conceivable that some as of yet unidentified action of the compound is causing the dose-limiting toxicity). Nonselective NMT inhibitors like compound 2 can be well tolerated at an efficacious dose to treat the first stage of HAT, a consequence of the high dependency of T. brucei on NMT. However, the higher doses often required to also effectively treat the second CNS stage of the disease have been poorly tolerated, as was the case for compound 3, a blood−brain barrier penetrant compound. We therefore deliberately sought compounds with maximal selectivity to reduce the possibility of target-driven toxicity and provide a suitable therapeutic window to achieve a fully curative dose regimen for stage 2 HAT. In the case of the pyrazole sulfonamide series, we identified the subpocket around the Leu421 as an area in which we could obtain selectivity. Leu421 is the C-terminal residue of TbNMT that interacts with the amine. We attempted to obtain an increase in selectivity by optimization of the amine but were only able to obtain a modest increase. 6 We were unable to rationalize how to build in selectivity, and this was achieved empirically. At the time of carrying out this work, there was no human NMT structure available. Comparison of multiple parasitic and human NMT structures showed the active sites to be highly conserved with minimal evidence of conformational change; hence, there was no design rational to be drawn to generate ligands with higher degrees of selectivity.

■ AIMS AND OBJECTIVES
The overall aim of the research was to develop lead compounds with a profile suitable for delivering a fully curative, well-tolerated dose regimen in the stage 2 mouse model of HAT. In this article, we discuss the identification of a novel scaffold with selectivity for TbNMT over HsNMT. Structural information and our existing knowledge of NMT inhibitors was then used to increase potency in two new subseries. Further, we were also able to combine the separate selectivity-enhancing elements of our previously developed pyrazole sulfonamide series with those of this new series to

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Article enhance the overall selectivity. We were also able to derive compounds with blood−brain barrier permeability.

■ RESULTS AND DISCUSSION
Identification of a TbNMT Selective Hit: Methyl Ester Series. Methyl ester 4 (Scheme 2), identified as a singleton by high throughput screening, was at least 10-fold more selective for TbNMT over HsNMT compared to the pyrazole sulfonamide hit 1, making it a potentially attractive start point for optimization. From previous work, a ratio of inhibition of the HsNMT potency compared to activity in a parasite growth assay (selectivity "S" = HsNMT IC 50 /T. brucei EC 50 ) was found to give a better indication of selectivity rather than direct comparison of potency at either enzyme. 6 "S" for 4 was also significantly improved (>8.3 compared to 1.0 for 1). We therefore sought to optimize 4 through exploration of its structure−activity relationships and through hybridization with key elements from the pyrazole sulfonamides with the aim of maximizing selectivity.
Initial work on SAR generated some interesting results, but it proved difficult to rationalize these until we had structural information. Although replacement of the azepane moiety of 4 with a pyrrolidine gave an equipotent compound 5, removal of the azepane group entirely resulted in loss of activity (7). Unexpectedly, piperidine analogue 9 was also inactive. Modifications to substituents on the phenylsulfonamide ring were tolerated (cf. 8). However, carboxylic acid 6 proved inactive (Scheme 2). This was particularly important because the methyl ester undergoes rapid hydrolysis in plasma, which would result in a complete loss of activity. The development of

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Article efficacious compounds therefore required the identification of stable ester bioisosteres capable of maintaining activity against NMT in vivo.
Use of Structural Information to Find the Binding Mode of the Methyl Ester Series. We were unable to obtain TbNMT crystal structures; however a structure was obtained for 4 bound to LmNMT:MCoA ( Figure 2A) as a surrogate showing the ligand could bind in the peptide binding groove. The main interactions were hydrogen bonds between the ester carbonyl and Ser330 and between a sulfonamide oxygen and Asp396 together with a stacking interaction between the lower phenyl ring and Tyr217. Figure 2B shows the overlay of 4 with pyrazole hit 1 showing that both molecules adopted twisted conformations due to their common sulfonamide motif and formed a similar key hydrogen-bonding interaction with Ser330.
A significant difference was observed, however, in the conformation of the protein when 4 was bound ( Figure 2C): there was a conformational change in residues 231−236, part of the DE loop, to alleviate a steric clash between the azepane ring and the side chain of Phe232. This loop is highly flexible as judged by crystallographic B-factors, allowing a 5 Å shift in the position of the Phe232 side chain. The flexibility is facilitated by the Gly234 residue, which is not present in the HsNMT protein. Hence, this could explain the high selectivity observed for this ligand with the LmNMT (and TbNMT) protein able to change conformation, whereas the HsNMT protein, being less flexible, suffers a steric clash preventing binding of the ligand. Furthermore, the azepane ring was observed to adopt a folded conformation not favorable for a piperidine ring, which might explain the lack of activity of the piperidine analogue (9). The pyrrolidine ring of 18 (a more decorated version of compound 5) can also adopt a similar folded conformation to the azepane of compound 4 in agreement with our selectivity hypothesis.
Use of Existing Knowledge of NMT Inhibitors to Increase Potency in the Methyl Ester Series. From our previous work, 5,6 appending a basic moiety to DDD64558 (1) to reach the C-terminal carboxylic acid gave an ∼1000-fold increase in potency (cf. compounds 2 and 3). 5,6 Using the crystallographic information, an amine-containing substituent was appended to the arylsulfonamide core of 4 to give 10 (Scheme 3) to place a basic nitrogen adjacent to the Cterminal carboxylic acid (Leu421). This substitution gave a marked increase in potency versus TbNMT and also maintained selectivity over HsNMT. A crystal structure of 10 bound to LmNMT:MCoA confirmed that the ligand retained the interactions of 4 with the protein, including the azepanegenerated conformational shift. Additional water-mediated interactions with the C-terminal carboxylic acid of the protein accounted for the 150-fold gain in potency ( Figure 3). Overlay with the crystal structure of 2 showed that the two ligands Compound 10 was also highly potent against the T. brucei parasite. However, the ester was rapidly hydrolyzed to the inactive acid (cf. 6) in mouse plasma (t 1/2 < 15 min; method in Supporting Information). Two approaches were therefore adopted to find an alternative stable framework, which is isosteric with the ester, to retain the hydrogen bond donor functionality important for binding to Ser330 (Scheme 4).
Biaryl Isostere Strategy to Increase Metabolic Stability. The first approach was to replace the ester with a pendent heterocyclic hydrogen bond acceptor to maintain the H-bond acceptor interaction with Ser330 (Scheme 5). A wide range of heterocycles (pyrazoles, imidazoles, thiazoles, oxazoles, etc.) were investigated. However, all compounds proved inactive in our biochemical assay except 3-pyridyl 11, which showed weak activity, ∼50-fold lower than that of the methyl ester analogue (cf. 8, Scheme 2).
Bicyclic Isosteres to Improve Metabolic Stability. A second approach was to explore the incorporation of a hydrogen bond acceptor into a fused bicyclic system (Scheme 6). It was noted that removal of the azepane or pyrrolidine groups from the methyl ester series (7, Scheme 2) resulted in loss of activity. However, to aid ease of synthesis, compounds without an azepane or pyrrolidine substituent were originally prepared. Of these, quinoline 12, indazole 13, and benzothiazole 14 showed weak activity against TbNMT. The quinoline and indazole hits were selected for further studies.
Reinstatement of the azepane group into the quinoline scaffold (22, Table 1) resulted in a compound with similar potency and selectivity to hit compound 4. Similarly, addition of a pyrrolidine group improved the potency of both quinoline and indazole systems (Scheme 7). The lactone analogue of the methyl ester (15) was inactive.
Quinoline Series. Appending an amine tail to 16 to reach the C-terminal carboxylic acid of NMT gave 18, resulting in the expected gain in potency while maintaining the selectivity observed with the methyl ester series. A crystal structure of 18 bound to LmNMT:MCoA ( Figure 4) showed that the binding mode of the ester series was retained with the quinoline nitrogen of 18 forming a similar hydrogen bonding interaction to Ser330 as the ester carbonyl of 4. The pyrrolidine ring of 18 occupies similar space to the azepane of 4, possibly explaining the retention of high selectivity.
Head Group Optimization. Compounds with an amine in the quinoline 6-position such as 16 were found to be chemically unstable with mass spectrometry suggesting that oxidation of the pyrrolidine ring under air was occurring. Hence, an array of 6-alkyl-substituted quinolines was prepared (Scheme 8, Table 1). Synthesis was achieved by Negishi coupling with bromide 19 to install the 6-alkyl group (20) followed by nitro reduction and coupling with 4-bromophenylsulfonyl chloride to form sulfonamide 21.
Removal of an electron-donating nitrogen by replacement of the 6-pyrrolidine with a 6-cyclopentyl group (24) resulted in a chemically stable compound with similar potency. However, substitution with a cyclohexyl or phenyl ring (25,26) resulted in loss of activity. This is consistent with the observed loss of activity for a piperidine substituent in the methyl ester series (9, Scheme 2). Benzyl and 3-pentyl groups were tolerated (29,30), but the best potency was obtained with isobutyl and cyclohexylmethyl groups (27,28). Of these, the isobutyl substituent (27) showed good potency and selectivity with the minimum of addition of molecular weight and lipophilicity, i.e., equal ligand efficiency (LE) 24 but improved ligand-lipophilicity Scheme 6. Activities of Fused Bicyclic Hydrogen Bond Acceptors against TbNMT

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Article efficiency (LLE), 25 and was thus selected for further optimization.
Appending an alkyl chain-linked piperidine "tail" (31 and 32, see Table 2) to the 6-isobutylquinoline system gave the expected improvement in potency in analogous fashion to that observed in the pyrazole and methyl ester series (cf. compounds 3 and 10). The crystal structure of 32 bound to LmNMT:MCoA showed that the overall binding mode of the series was retained with the 6-isobutyl group sufficient to induce the conformational change in residues 231−236 that is proposed to confer selectivity over HsNMT ( Figure 5).
Strategy to Increase Selectivity in the Quinoline Series by Tail Group Optimization. It was known from our previous work in the pyrazole sulfonamide series that selectivity could be improved by optimization of the aminecontaining "tail group" moiety. 6 Hence, combining the selectivity conferred by the quinoline isobutyl substituent with that conferred by different terminal amines was attempted. An array of alternative pendant amines was then prepared in the search for more selective compounds ( Table  2). The amine moieties were appended by first hydroboration of an appropriate alkene precursor with 9-BBN followed by

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Article Suzuki coupling of the crude borane with bromide 27 (Scheme 9). 26,27 In some cases, a t-Boc-protected amine precursor was used. These t-Boc-protected products were converted into secondary amines by cleavage of the t-Boc group with TFA or into N-methyl amines by cleavage of the t-Boc group followed by reductive amination with paraformaldehyde or directly by reduction of the t-Boc group with lithium aluminum hydride.
However, by far the most selective compound was the pseudotropine-derived compound 41, which was nearly 4000fold selective (enzyme selectivity 3900; "S" = 325). Interestingly, the analogous tropine-derived compound (42) was much less selective (albeit more potent). The pseudotropine group was known to confer modest selectivity in the pyrazole series (cf. DDD101140; Figure 6). 6 Therefore, we had established, at least in this case, that a synergistic combination of selective "head" (the isobutylquinoline) and "tail" (the pseudotropine) groups resulted in a compound with extremely high selectivity. The origin of the contribution to selectivity, which the pseudotropine group makes, is unknown. A crystal structure of this ligand bound to LmNMT could not be obtained. The region of the active site around the Cterminus is highly conserved between species with no obvious differences that could explain differences in selectivity. It is possible that the particular size and shape of the pseudotropine ligand can be better accommodated in the T. brucei enzyme due to differences in protein flexibility or the stability of the water molecules that form the hydrogen bonding network.
In general, these compounds had lower potency against the T. brucei parasite than the pyrazole series (e.g., compound 3). This is in keeping with lower potency against the TbNMT enzyme (2−16 nM for the quinoline series against typically <2 nM for the pyrazole series).
Indazole Series Headgroup Optimization. A 5pyrrolidinylindazole, 17 (Scheme 7), had also shown some activity against TbNMT, and a series of indazoles were also evaluated as a quinoline surrogate. A limited array of 5alkylindazoles was prepared based around the most successful compounds in the quinoline series (Table 3) using a similar chemical route (Scheme 10). An isobutyl group (51) again resulted in good potency and selectivity, as did a methylcyclohexyl group (52). In this series, these two compounds showed at least 10-fold better potency than those compounds with a cyclic group appended directly to the indazole.
Tail Group Optimization to Increase Selectivity in the Indazole Series. The tail group amine was then optimized in analogous fashion to the quinoline series (Table 4). Synthesis was performed using the same hydroboration-Suzuki coupling protocol utilized for the quinoline series (Scheme 11). Groups that gave good selectivity in the quinoline series (Table 2) also performed well in the indazole series, often with improved selectivity over the analogous quinoline. Six compounds were found to have greater than 500-fold selectivity for TbNMT over HsNMT (55, 57, 58, 60, 61, and 62) with the pseudotropine-derived compound 60 again showing the greatest selectivity ("S" = 318). A crystal structure of the 5isobutyl indazole ligand 54 bound to LmNMT:MCoA showed the binding mode to be comparable to the 6-isobutyl quinoline with the indazole N1 lone pair forming the key H-bond to Ser330 (Figure 7). The binding mode of the remainder of the molecule is highly conserved with all key interactions described previously retained. The indazole series showed better potency against T. brucei than the quinoline series in keeping with its higher potency against the TbNMT enzyme.
DMPK and in Vivo Efficacy. DMPK data for key compounds are shown in Table 5. Compounds demonstrated a range of mouse microsomal intrinsic clearance with examples  Table 1 for the different "R" groups: compounds 22−30).

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Article from both indazole and quinoline series having acceptable metabolic stability (i.e., Cli <5 mL min −1 g −1 ). Metabolite identification studies on one of the more metabolically unstable quinoline series compounds, 32, following incubation with mouse liver microsomes, suggested that oxidation of the central phenyl ring, or of the alkyl chain linker, predominated rather than N-demethylation or isobutyl group metabolism. Both series also demonstrated moderate to low plasma protein binding. One major concern was hERG inhibition with both series demonstrating low micromolar inhibition, potentially driven by the basic, terminal amine group. Standard techniques to reduce hERG inhibition are to lower lipophilicity and basicity. 28,29 To this end, a series of compounds bearing a morpholine group were prepared, as this gave compounds with the desired lower logD and pK a (See Figure 8). No significant improvement in hERG activity was observed, and a substantial penalty was paid in unacceptably high metabolic instability.
Because of its markedly superior EC 50 against the T. brucei parasite and acceptable metabolic stability (Cli), compound 54 was selected for progression toward a stage I mouse efficacy model of HAT. When dosed orally at 50 mg/kg, 54 demonstrated good exposure (AUC 0−8 = 2,900,000 ng-min/ mL; C max = 11,000 ng/mL; T max = 1 h) and in keeping with its high "S" value (47), 54 was well-tolerated (maximum tolerated dose >300 mg/kg when dosed twice a day orally for 3 days). In the stage 1 HAT mouse model, with female NMRI mice infected with T. brucei s427, compound 54 was fully curative at 10 mg/kg when dosed twice a day orally for 4 days, starting

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Article day 3 postinfection. All animals relapsed at dose levels of 0.3, 1, or 3 mg/kg orally twice daily for 4 days. Assuming linearity of exposure, the free C max at the minimal efficacious dose (10 mg/ kg) would only give a cardiovascular window of ∼10-fold, highlighting a significant issue for progression for this series unless hERG liability could be reduced while maintaining high potency.
The ability to cross the blood−brain barrier is essential for treatment of stage 2 HAT, when the parasites are present in the central nervous system. Compounds in the quinoline series were brain penetrant but unfortunately lacked the right balance of potency and metabolic stability for progression. Furthermore, the hERG inhibition was marginally higher within this series. In contrast, the indazole series was very poorly brain penetrant, and for 54, the most profiled exemplar from the series, the observed very poor brain penetration was demonstrated to be due to the fact it was a strong Pglycoprotein substrate. Following coadministration in rat with the Pgp inhibitor, GF120918, the brain:blood ratio for 54 increased to 2.2 (0.09 without GF120918 coadministration).
Validation of NMT as a Drug Target for Stage 2 HAT. In previous studies, we had validated NMT as a drug target in stage 1 HAT. 4−6 However, these molecules were not brain penetrant or did not offer sufficient tolerability in rodents to be able to deliver a dosing regimen to conclusively validate NMT as a viable drug target in stage 2 HAT. Owing to its high potency against the T. brucei parasite, good oral pharmacokinetics and excellent tolerability, 54 appeared to be a good tool molecule to validate NMT for stage 2 HAT. For the purposes of the stage 2 HAT model, the poor CNS penetration of 54 could be overcome by using CF-1 mdr1a deficient mice. 30 Using this model, a completely curative dosing regimen was obtained for 54 ( Figure 9) at 50 mg/kg orally twice daily for 5 days, validating NMT as a suitable drug target for stage 2 HAT. Using female NMRI mice, not mdr1a deficient, compound 54 relapsed at all dose levels (50, 100, and 200 mg/kg orally twice daily for 5 days) in the stage 2 model.
We have previously shown that poor CNS penetration could be overcome by application of a metabolically stable difluoromethyl "cap" to the sulfonamide. 6 Pleasingly, when this modification was made to indazole 54 (i.e., 66, see Scheme 12) the brain:blood ratio was significantly improved (1.9 vs < 0.1), whereas other properties were largely left unaffected, including trypanocidal activity, metabolic stability (mouse Cli = 2.3 mL/min/g), selectivity "S" and also unfortunately hERG (IC 50 = 6 μM). Consequently, because of the likely unacceptable cardiovascular safety window (this was only ∼3-fold at 50 mg/kg, delivering stage 2 efficacy for 54), 66 was not progressed further.

■ CONCLUSIONS
We show here that for the first time it is possible to obtain inhibitors of TbNMT that are highly potent, selective, and CNS penetrant (i.e., 41 and 66). The substantial gains in selectivity achieved relative to earlier series has resulted from the additive effect of combining selectivity-enhancing pendent amines from a previous series with new highly selective head groups, which translates into improved tolerability in vivo.
High-throughput screening identified 4, an inhibitor of TbNMT, with selectivity over HsNMT. A chemistry campaign based around crystal structures of the ligand bound to LmNMT led to the discovery of two novel alternative scaffolds: one based on a quinoline and one based on an indazole. Both scaffolds showed good potency and selectivity for the parasite enzyme. From crystallographic studies of our previous pyrazole series, we were able to hybridize these  Table 2 for the different "R" groups: compounds 31−45; in some cases, an additional synthetic step was required, see the Supporting Information for details).

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Article scaffolds with our previous pyrazole series leading to compounds with potent activity against TbNMT and greater than 1000-fold selectivity for TbNMT vs HsNMT and with "S" values ranging from 10-up to 300-fold. Although some of these compounds had antitrypanocidal activities with EC 50 < 100 nM, in general they were less potent than the pyrazole series with which we had previously worked. Some of the quinoline series showed blood−brain barrier penetration, but none had the right balance of potency against the parasite, metabolic stability, and selectivity to progress further. In contrast, one of the indazole compounds, 54, had good potency against the T. brucei parasite (EC 50 = 5 nM), good microsomal stability (Cli = 2.9 mL/min/g), good selectivity ("S" = 47), good oral exposure, and good in vivo tolerability. It was efficacious in a stage 1 mouse model of HAT and could potentially be further developed as a drug to treat stage 1 of the disease, except the cardiovascular toxicity window was too low because of the hERG liability. Although, compound 54 lacked sufficient brain penetration for treating stage 2 HAT as a consequence of strong Pgp interaction, a fully curative oral dose regimen was achieved in the stage 2 GVR35 mouse model of HAT when using CF1 mdr1a deficient mice for the infection. This validates NMT as a drug target for stage 2 HAT. It remains to be seen whether this series offers the potential to deliver a lead with all the properties required for further development toward a treatment for both stages of HAT, particularly with regard to a molecule potent against the parasite, having good pharmacokinetics and brain penetration and an acceptable cardiovascular safety window.

■ EXPERIMENTAL SECTION
General Experimental Information. Chemicals and solvents were purchased from the Aldrich Chemical Co., Fluka, ABCR, VWR, Acros, Fisher Chemicals, and Alfa Aesar and were used as received unless otherwise stated. Air-and moisture-sensitive reactions were carried out under an inert atmosphere of argon in oven-dried glassware. Analytical thin-layer chromatography (TLC) was performed on precoated TLC plates (layer 0.20 mm silica gel 60 with fluorescent indicator UV254 from Merck). Developed plates were airdried and analyzed under a UV lamp (UV254/365 nm). Flash column chromatography was performed using prepacked silica gel cartridges (230−400 mesh, 40−63 μm, from SiliCycle) using a Teledyne ISCO Combiflash Companion or Combiflash Retrieve. 1 H NMR spectra were recorded on a Bruker Avance II 500 spectrometer ( 1 H at 500.1 MHz) or a Bruker DPX300 spectrometer ( 1 H at 300.1 MHz). Chemical shifts (δ) are expressed in ppm recorded using the residual solvent as the internal reference in all cases. Signal splitting patterns are described as singlet (s), doublet (d), triplet (t), quartet (q), multiplet (m), broad (b), or a combination thereof. Coupling constants (J) are quoted to the nearest 0.1 Hz. LC-MS analyses were performed with either an Agilent HPLC 1100 series connected to a Bruker Daltonics MicrOTOF or an Agilent Technologies 1200 series HPLC connected to an Agilent Technologies 6130 quadrupole LC-MS, where both instruments were connected to an Agilent diode array detector. All assay compounds had a measured purity of ≥95% as determined using this analytical LC-MS system (TIC and UV). Highresolution electrospray measurements were performed on a Bruker Daltonics MicrOTOF mass spectrometer. Microwave-assisted chemistry was performed using a Biotage Initiator Microwave Synthesizer. Purity (>97%) and molecular mass were confirmed by HPLC and high-resolution mass spectrometry.

Journal of Medicinal Chemistry
Article nitroquinoline. 6-Bromo-5-nitroquinoline (0.378 g, 1.49 mmol), Pd(dppf)Cl 2 ·CH 2 Cl 2 (66 mg, 0.08 mmol, 5 mol %), and copper(I) iodide (48 mg, 0.25 mmol, 17 mol %) were added to a dry 50 mL flask under argon. The flask was purged three times with a vacuum/ argon cycle, and DMF (12 mL) was added. The mixture was stirred at room temperature, and the flask was purged a further three times with a vacuum/argon cycle. The mixture was cooled to 0°C, and a 0.5 M solution of the isobutylzinc bromide in THF (4.5 mL, 2.25 mmol, 1.5 equiv) was added dropwise. The mixture was then heated at 80°C overnight.
The reaction mixture was then concentrated, treated with EtOAc (20 mL) and water (20 mL), and stirred vigorously for 30 min. The mixture was filtered; the phases were separated, and the aqueous phase was extracted with further EtOAc (2 × 20 mL). The combined organic extracts were washed with water and brine, dried (Na 2 SO 4 ), filtered through a plug of SiO 2 , and concentrated. The products were purified by flash chromatography on silica (0−100% EtOAc-hexane) to give the title compound as a brown gum (311 mg, 1.43 mmol, 96%). 1  Prototypical Procedure for the Hydrogenation of an Aryl Nitro Group to an Amine. 6-Isobutylquinolin-5-amine. A solution of 6-isobutyl-5-nitro-quinoline (286 mg, 1.42 mmol) in EtOAc (10 mL) was subjected to hydrogenation conditions with 5%Pd/C (93 mg) under a hydrogen balloon at room temperature for 6 h. The reaction mixture was filtered through Celite, and the filtrate was concentrated to give the crude title compound (285 mg, 1.19 mmol, 92%) as a brown solid, which was used without purification. LCMS (ES + ) [M + H] + m/z 201.1.
Prototypical Procedure for the Coupling on an Aryl Amine with a Sulfonyl Chloride. 4-Bromo-N-(6-isobutylquinolin-5-yl)benzenesulfonamide (27). 5-Amino-6-isobutylquinoline (220 mg, 1.10 mmol) was taken up in a mixture of pyridine (2 mL) and DCM (3 mL); 4-bromobenzene-1-sulfonyl chloride (337 mg, 1.32 mmol) was added, and the reaction mixture was stirred at room temperature for 3 days. The solvent was removed under reduced pressure; the residue was partitioned with DCM and saturated aqueous sodium bicarbonate solution, and the organics were dried (MgSO 4 ) and concentrated to dryness. Purification by flash chromatography (0− 100% EtOAc-CH 2 Cl 2 ) gave the title compound (299 mg, 0.71 mmol, 65%) as a cream solid. 1   and potassium phosphate (53 mg, 0.25 mmol) in DMF (2 mL) and water (0.6 mL) under argon. After bubbling argon through the reaction for 5 min at room temperature, Pd(dppf)Cl 2 ·CH 2 Cl 2 (16 mg, 0.02 mmol, 10 mol %) was added, and the reaction vessel was sealed and heated in a microwave at 90°C for 1 h. The reaction mixture was concentrated under reduced pressure, resuspended in CH 2 Cl 2 , and applied to a 2 g SCX-2 column. The column was washed with CH 2 Cl 2
T. brucei cell viability assays were carried out as described previously. 6 In brief, bloodstream T. b. brucei s427 was cultured at 37°C in modified HMI9 medium (56 μM 1-thioglycerol was substituted for 200 μM 2-mercaptoethanol) and quantified using a hemocytometer. Cell culture plates were stamped with 1 μL of an appropriate concentration of test compound in DMSO followed by the addition of 200 μL of trypanosome culture (10 4 cells ml −1 ) to each well except for one column that received media only. Culture plates of T. brucei cells were incubated at 37°C in an atmosphere of 5% CO 2 for 69 h before the addition of 20 μL of resazurin (final concentration, 50 μM). After a further 4 h incubation, fluorescence

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Article was measured (excitation 528 nm; emission 590 nm) using a BioTek flx800 plate reader.