Total Synthesis of Xanthoangelol B and Its Various Fragments: Toward Inhibition of Virulence Factor Production of Staphylococcus aureus

As an alternative strategy to fight antibiotic resistance, two-component systems (TCSs) have emerged as novel targets. Among TCSs, master virulence regulators that control the expression of multiple virulence factors are considered as excellent antivirulence targets. In Staphylococcus aureus, virulence factor expression is tightly regulated by a few master regulators, including the SaeRS TCS. In this study, we used a SaeRS GFP-reporter system to screen natural compound inhibitors of SaeRS, and identified xanthoangelol B 1, a prenylated chalcone from Angelica keiskei as a hit. We have synthesized 1 and its derivative PM-56 and shown that 1 and PM-56 both had excellent inhibitory potency against the SaeRS TCS, as demonstrated by various in vitro and in vivo experiments. As a mode of action, 1 and PM-56 were shown to bind directly to SaeS and inhibit its histidine kinase activity, which suggests a possibility of a broad spectrum inhibitor of histidine kinases.


■ INTRODUCTION
Antibiotic resistance has arisen as an extremely serious health problem in all parts of the world. New resistance mechanisms are emerging and spreading globally, threatening our ability to treat common infectious diseases. The list of resistant infections−such as pneumonia, tuberculosis, blood poisoning, gonorrhea, and foodborne diseases−is growing longer and sometimes these are impossible to treat as antibiotics become less effective. 1,2 It is estimated that, by 2050, worldwide drugresistant infections will claim 10 million deaths annually. One particularly problematic pathogen is methicillin-resistant Staphylococcus aureus (MRSA). MRSA is a nosocomial and communal menace that is resistant to many antibacterial drugs and antiseptics. 3−5 The U.S. Centers for Disease Control and Prevention recently issued a report to various governments and organizations outlining the concern about MRSA. This report referenced the New Drugs for Bad Bugs (ND4BB) project initiated by the European Union, the United States military, and the World Health Organization (WHO). 6,7 The Antimicrobial Resistance Global Report on Surveillance released by the WHO in 2014 outlined a global initiative to tackle the MRSA superbug and rekindle a campaign for new, effective, and safe drugs. 8−10 Since they suppress bacterial growth, conventional antibiotics are confined to a limited lifespan. It is urgent to establish a new path forward for medicinal chemists to address successfully this problem.
Among many new approaches, the inhibition of virulence factors has shown promising outcomes. This strategy bypasses the route of killing pathogens and thereby has the potential to suppress the development of resistance. Many such concepts have been tested. 11−13 Among these, two-component systems (TCSs) that control virulence factors have been of particular interest. 14−17 Some TCSs control multiple virulence factors in pathogens and are thus called master virulence regulators. 18 These TCSs are attractive targets for novel antivirulence

Journal of Medicinal Chemistry
Article agents. We identified one such TCS, the SaeRS system of S. aureus, as an excellent antivirulence target that is less likely to evolve resistance. 19 We developed a GFP reporter system that can be applied to a high-throughput assay format. Using this assay, we discovered certain FDA-approved drugs that exert antivirulence effects on S. aureus. 20 In continuing such studies, we identified natural compound inhibitors of the SaeRS TCS by screening plant-derived products 21 using the same assay. Of the identified natural products that showed promising inhibitory activity, we chose xanthoangelol B for further study including the synthesis of complete xanthoangelol B, fragments, and a derivative, to test for antivirulence activities.
Xanthoangelol B is a prenylated chalcone isolated from Ashitaba (Angelica keiskei Koidzumi, Apiaceae). The Japanese herb Ashitaba is a perennial plant found along the Pacific coast of Japan. Ashitaba is enriched with numerous active compounds such as coumarins, flavanones, and chalcones. 22−25 Xanthoangelol B is relatively scarce among these compounds, although it is found in all parts of the plant. 26 It shows various therapeutic properties, such as antioxidative activity, antihypertensive activity, anti-inflammatory activity, and antiviral activity. 27 However, although its related chalcones, xanthoangelol and 4-hydroxyderricin, were shown to have an antibacterial activity against Gram-positive bacteria, 28 its antibacterial activity has not been established. Thus, given our intriguing screening results, we set out for an in-depth structure−activity study of xanthoangelol B. We were particularly interested in its effect on the virulence of S. aureus and its feasibility as an antivirulence agent.
Xanthoangelol B has a modular structure composed of chalcone and isoprene moieties. It was deemed important to analyze the biological activity of its fragments to establish any structure−activity relationships. Thus, we synthesized frag-ments of xanthoangelol B along with the parent compound and tested their activities. During the synthesis, we obtained a derivative of xanthoangelol B (compound PM-56) that had a comparable activity and tested its activities. Here, we report these processes and describe the antivirulence activities of the compounds that we synthesized.
Our synthetic design involved the limited use of protecting groups (Scheme 2). Our initial approach was to introduce 2,6dimethylhepta-1,6-dien-3-ol at the end of the synthesis to reduce complications arising from the chiral aliphatic hydroxyl group. The 4′-hydroxyl position of 2′,4′-dihydroxyacetophenone was alkylated with allyl bromide that was heated at 220°C in N,N-diethylaniline for 24 h. This allowed for Claisen rearrangement, giving a 3,3-sigmatropic-shifted major product 10 with a 76% yield. The minor product was 5-allyl-2,4-

Journal of Medicinal Chemistry
Article dihydroxy acetophenone. The regioselectivity of Claisen rearrangement giving a 3-allylated major product is well documented. 32 Compound 10 was then subjected to aldol condensation by refluxing for 18 h in KF-alumina (40%), a solid-support catalyst in toluene. Compound 11 was obtained as the major product, with a yield of approximately 60%. This yield was slightly lower than that of most aldol condensation reactions owing to the two 2′,4′-OH groups. A reaction longer than 18 h resulted in the formation of a nonpolar mixture of spots observed by TLC analysis. The nature of the solvent also drastically influenced the yield of the reaction. 2,6-Dimethylhepta-1,6-dien-3-ol was introduced later by initially synthesizing 2,2-dimethyl-3-(3-methylbut-3-en-1-yl)oxirane 8 from citral by subjecting it to decarbonylation via heating at 130°C in the presence of catalytic amounts of palladium acetate (10 mol %) in a sealed tube for 24 h. This produced 2,6-dimethylhepta-1,5-diene 12, with a yield of ca. 62%. 33 Dimethylhepta-1,5-diene was subjected to selective oxidation using m-chloroperbenzoic acid (mCPBA) to obtain an epoxide 8 with a 45% yield. 34 Compound 8 was then subjected to cross-olefin metathesis with 11, using a Hoveyda−Grubbs second-generation catalyst to yield 70% of an E-product 13. 35 The epoxide ring was subsequently subjected to selective ring opening reactions or isomerization using in situ-generated diethylaluminum 2,2,6,6-tetramethyl-piperidide to yield the desired product xanthoangelol B 1 at a 69% yield. 36 There were a few interesting observations during the synthesis of 1 (Scheme S1). As an alternative route to xanthoangelol B, the introduction of 2,6-dimethylhepta-1,6dien-3-ol to the 3′-position of 2,4-dihydroxyacetophenone resulted in decreased reactivity toward condensation reactions. Use of a solid-support reagent (such as KF-alumina) or use of highly basic conditions yielded no product or a complex mixture of products. Heating the reaction in the presence of a high boiling-point solvent (either in highly basic or acidic conditions) yielded no desired product. Finally, the use of Lproline as an organic catalyst 37,38 resulted in an aldol addition product 15. However, this product did not react further (to elimination) to yield 1 in any of the acidic or basic conditions attempted. Thus, 15 was subjected to selective epoxide isomerization, and the resulting compound PM-56 was tested for antivirulence activities as a derivative of 1.
Synthesized xanthoangelol B 1 was structurally identical to the natural product, except for the chiral center. Selective epoxidation and subsequent epoxide ring opening resulted in an enantiomeric mixture. We speculated that the number of required steps to synthesize 1 could be reduced by using a longer chain (i.e., geranyl bromide) and carrying out subsequent selective epoxidations, which could be reacted further to yield 1. This would provide us with an additional fragment for structure−activity tests and allow us to incorporate the metal-free chemistry. Thus, we devised a shorter synthetic route (without using metal catalysts) by minimizing the use of protecting groups. Specifically, 2,4dihydroxy acetophenone was subjected to aldol condensation to give an intermediate that was reacted with 1,2-epoxy geranyl bromide. This generated 13, which underwent selective epoxyisomerization to give 1 as an enantiomeric mixture (Scheme 3). Then, we attempted an optical resolution of synthesized compound 1. We chose a method of enantioselective organocatalyst oxidative kinetic resolution using the 7-Bn-3n-Bu-4-oxa-5-azahomoadamantane/trichloroisocyanuric acid (TCCA) system to yield a resolved 1. 39 The 1 H and 13 C NMR spectra of the resolved compound 1 were in agreement with spectra previously reported. 21 The mass spectrum showed a peak at 409.19 corresponding to [M + H] + , consistent with the calculated mass of the desired product. In a direct comparison using HPLC, the synthetic and resolved 1 was shown to elute at exactly the same retention time as the natural product ( Figure S1). The optical rotation for kinetically resolved 1 was α D = +13.5 (MeOH, c = 0.5). This is consistent with the data reported for naturally extracted 1, 23 which indicates the authenticity of the kinetically resolved synthetic xanthoangelol B 1.
Biological Evaluation of Xanthoangelol B, Its Fragments, and Its Derivative. Previously, we constructed a SaeRS GFP reporter strain based on the S. aureus USA300 isolate and found promising leads among FDA-approved drugs through a high throughput screening. 20 The same SaeRS GFP reporter strain was constructed for screening a natural product library in this study. Briefly, as the α-hemolysin promoter (Phla) is a well-characterized target of theSaeRS TCS, we generated a minimal Phla, termed Phlam, which contains only the SaeR binding sites and the −10/−35 promoter sequences of the hemolysin gene (hla). Phlam was fused to a green fluorescence protein (gfp) gene instead of hla in the singlecopy integration plasmid pCL55. The resulting plasmid (pCL-Phlam-gfp) was inserted into S. aureus USA300. Hence, if the expression of GFP in a sample was lower than the control sample, it meant that the SaeRS TCS was inhibited. As the complete inhibition of Phlam requires a complete shutdown of the SaeRS TCS (such as a deletion of saeS), Phlam will detect only potent inhibitors of the SaeRS reporter. Xanthoangelol B 1 was one of the hits among the natural products screened with this method. Thus, we assayed 1, its fragments, and a derivative (PM-56). The structures of all the assayed compounds and fragments are presented in Scheme 4 and Scheme S2.

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Article Structure−Activity Studies of Xanthoangelol B and Its Inhibitory Effect on the SaeRS TCS. On initial screening, 1 and PM-56 showed an excellent inhibitory activity against SaeRS. These compounds repressed GFP expression while allowing bacterial growth, making them ideal antivirulence candidates. In contrast, all of the fragments (Scheme S2) failed to inhibit the SaeRS TCS, even at 20 μM. This indicates that the whole structure is needed for the inhibitory activity. Furthermore, xanthoangelol and xanthoangelol F (Scheme 4) did not show inhibitory activity at 20 μM.
These two analogs are also natural prenylated chalcones from Ashitaba, but they lack a hydroxyl group in the terminal isoprene unit. This hydroxyl group appears to be crucial for the antivirulence activity of this class of compounds. Xanthoangelol with an identical structure to that of 1, except for this hydroxyl group, did not have any activity (although methoxylation in the central phenol hydroxyl group of xanthoangelol F may still have an impact). IC 50 values against the SaeRS GFP reporter were measured to be 2.1 μM for 1 and 4.3 μM for PM-56. In these experiments (at up to 8 and 16 μM), 1 and PM-56 did not disrupt bacterial growth ( Figure  1A,C). To elaborate the inhibitory effect of both compounds on the growth of S. aureus, we measured the growth of S. aureus in the presence of 1 and PM-56 at higher concentrations up to 20 h. From 16 μM, 1 started showing growth inhibition, and at 64 μM of 1, the growth of S. aureus was severely inhibited ( Figure S2). However, up to 64 μM of PM-56, S. aureus still showed comparable growth to the control ( Figure S2). The trends shown at 8 h did not change up to 20 h. This indicates that the rigidity conferred to the chalcone structure by the conjugated double bond is not essential for anti-SaeRS activity but may lead to higher toxicity (which affects bacterial growth). Together, these data strongly indicate that the isoprene and chalcone moieties are necessary in their entirety for the inhibitory activity.
Effects of Inhibitors on Downstream Virulence Factors. Staphylococcal α-hemolysin, which causes lysis of erythrocytes, is the target virulence factor of the SaeRS TCS. Therefore, if the SaeRS TCS is inhibited, the expression of αhemolysin should decrease to a certain degree. When 1 and PM-56 were incubated with erythrocytes in the presence of methicillin-resistant S. aureus USA300, notable protection from hemolysis was observed ( Figure 2 and Figure S3). At only 4 μM, both compounds showed visible protection. At 8 μM, a comparable level of protection to that of saeS knockout mutants (SaeS::Tn551) was observed. The hemolysis activity of this saeS mutant was reduced to 82.6% in comparison to the wild type S. aureus. To confirm the relevance of the protection effect observed with 1 and PM-56 to the SaeRS pathway, we also tested 1 and PM-56 against the saeS knockout mutant. This experiment conducted at 8 μM of 1 and 15 μM of PM-56 showed the reduction in hemolysis by 86.9% and 87.4%,

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Article respectively, compared to hemolysis by the wild type ( Figure  S4). Although apparent further inhibition appeared to exist, the differences were not statistically significant, which thus suggests that the protection against hemolysis by 1 and PM-56 is highly likely attributed to inhibition of the SaeRS pathway. The IC 50 values of 1 and PM-56 for the inhibition of erythrocyte hemolysis were then measured to be 4.6 and 4.0 μM, respectively. These values were consistent with the IC 50 values of both compounds for the SaeRS TCS GFP reporter. Therefore, these strongly suggests that the protection of erythrocytes from hemolysis in the presence of MRSA can be attributed to inhibition of the SaeRS TCS.
We next analyzed the transcription of virulence genes in the presence of 1 and PM-56 ( Figure 3). We used concentrations of 8 μM (1) and 10 μM (PM-56), corresponding to an IC 90 value for the SaeRS GFP reporter. We observed significant transcriptional suppression of the following genes: α-hemolysin (hla), aureolysin (aur), γ-hemolysin, and staphylokinase. These four genes have a direct SaeR binding site in their promoter regions and are tightly regulated by the SaeRS TCS. 40 While it has been reported that hla is regulated by an accessory gene regulator (agr) as well as by sarA, a direct connection between these regulatory systems and the expression of hla remains under debate. 41 Their suppression likely reflects the inhibitory effects of both compounds on the SaeRS TCS. Then, we analyzed the transcription of a gene encoding Staphylococcal accessory regulator (SarA). SarA is responsible for the biofilm formation and not regulated by saeS. Thus, it was considered a good negative control, and its transcription analyzed post treatment of xanthoangelol B 1 and PM-56 ( Figure S5). As anticipated, it can be seen that the level of sarA transcription was not significantly affected by the two compounds. These results imply that 1 and PM-56 indeed inhibited the SaeRS pathway with specificity and thus are promising candidates for future development of antivirulence agents or chemical probes specific for the SaeRS two-component system.
Cytotoxicity. In order to explore the potential of xanthoangelol B 1 and PM-56 as initial leads for further

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Article translation, we have examined the cytotoxicity of both compounds on HeLa cells, quantitatively and qualitatively. First, LDH release assay was performed, in which we have treated HeLa cells with 1 and PM-56 at various concentrations (2−128 μM) for 72 h and measured the optical density at 450 nm. It was shown that IC 50 values for HeLa cell survivals were 19.70 and 28.94 μM with 1 and PM-56, respectively ( Figure  4A,B). These values were 10-fold and 7-fold higher than IC 50 values for the GFP-reporter inhibition by 1 and PM-56, respectively. Hence, the toxicity/efficacy ratio is reasonable for the initial leads. Next, the live/dead imaging experiment has been performed. The HeLa cells were seeded in 96-well plates for 24 h, and after attachment of cells, the culture media (DMEM, 10% FBS, 1% penicillin/streptomycin) were changed, followed by treatment with 1 and PM-56 at various concentrations (from 2 to 64 μM) for 72 h. The HeLa cells stained with fluorescein diacetate dye (live cell staining, green color) and propidium iodide dye (dead cell staining, red color) were observed using the confocal microscopy (Figure 4 C,D).
Confocal images were in accordance with the quantitative results as mentioned earlier. Both compounds at their IC 90 (approximately 8 μM) for the GFP-reporter did not produce substantially visible toxicity to the HeLa cells, while a reduction in viability was observed only when the cells were treated with concentrations higher than IC 50 for cytotoxicity.
Cytotoxicity tests demonstrated that 1 and PM-56 indeed had the promising traits as initial leads.
Validation of the Antivirulence Activity of Compounds 1 and PM-56 Using a Worm Infection Model. With the encouraging in vitro data, we further validated the antivirulence activity of 1 and PM-56 in a Galleria mellonella infection model. G. mellonella worms possess both a humoral and an innate immune system. The worms provide an attractive, simple, and easy-to-handle infection model with few biosafety or ethical issues. The G. mellonella infection model has previously been used with several human pathogens to test the in vivo efficacy of antibacterial and antivirulence drugs. 42 Thus, G. mellonella larvae was challenged with 5 × 10 6 colony forming units (CFUs), a load designed to allow only 20% survival after 72 h. The larvae was post-treated with various doses of either 1 or PM-56 at 12 h intervals, up to 72 h. Larvae health indices were strictly followed and recorded. A dose-dependent protection from virulence by both compounds (1 and PM-56) was observed in the larvae ( Figure 5). Compared to the vehicle control, 1 and PM-56 generated a 3.5-fold higher survival at doses as low as 2.0 and 2.1 mg/kg, respectively. Compound 1 showed 90% survival at a dose of 3.1 mg/kg and complete survival at 4.1 mg/kg. PM-56 showed 90% survival at 3.2 mg/kg and complete survival at 4.3 mg/kg. These survival rates were comparable to or slightly better than

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Article survival rates observed in worms infected with saeS knockout strains.
Next, we measured bacterial burden in the host worms. As shown in Figure S4, the inactivation of saeS resulted in a decline in the bacterial burden in the host worms (up to 1.7 log 10 CFU/worm). Treatment with 1 or PM-56 significantly lowered the bacterial burden in worms in a dose-dependent manner. Compared to the vehicle control, a dose of approximately 1 mg/kg allowed a sharp decrease in the bacterial burden. At concentrations of 4.1 and 4.3 mg/kg, respectively, 1 and PM-56 generated a decline of 1.72 log 10 and 1.84 log 10 CFU/worm ( Figure S6). Both compounds again showed comparable efficacy to that of saeS knockout. These results are highly consistent with survival tests. Then, to evaluate whether the activities of 1 and PM-56 were based on the saeS-dependent pathway, we have measured the effects of both compounds on G. mellonella infected with the saeS knockout mutant under the same conditions as above. G. mellonella were challenged with 5 × 10 6 CFU of the wild type and the saeS knockout S. aureus, which were subsequently treated with 1 (2.042 mg/kg body weight) or PM-56 (2.131 mg/kg body weight) for every 12 h interval for 72 h. These concentrations were corresponding to IC 90 for the SaeRS GFP reporter. The survival rates of G. mellonella infected with the knockout strain treated with 1 and PM-56 were 70% and 90%, respectively, while they were 20% for the wild type-infected larvae and 80% for the knockout strain-infected larvae without compounds ( Figure S7A), which showed protection effects were comparable. Then, we counted CFU from G. mellonella of each group after a 72 h infection. It was shown that CFU was significantly decreased in the saeS knockout group as compared to the wild type infection group by up to 74.5%. Compounds 1 and PM-56 treated groups also showed comparable reduction in CFU by 76.8% and 83.3%, respectively ( Figure S7B). Together, these results suggest that the protection effects initiated by the saeS knockout were not drastically improved by the treatment with 1 and PM-56, and subsequently, the activities of both compounds on G. mellonela were relevant to

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Article the saeS-dependent pathway although we cannot rule out an extra activity of PM-56 as the effects of PM-56 consistently showed a slight but nonsignificant improvement over the saeS knockout.
Overall, the superb protection given by 1 and PM-56 and the sharp decline in the bacterial burden following their use allowed us to draw comparisons with saeS knockout experiments. This hinted at a mode of action for these compounds. Thus, we next tested 1 and PM-56 for their ability to inhibit the histidine kinase or phosphotransferase activities of SaeS.
Effects of Compounds 1 and PM-56 on the Histidine Kinase and Phosphotransferase Activities of SaeS. The sensor histidine kinase (HK) of a bacterial two-component system represents a promising antibacterial target, as the catalytic and ATP-binding (CA) domain of the sensor HK is well conserved across bacterial species. Thus, an inhibitor binding this domain has the potential to be a broad-spectrum antibacterial agent. 15 Indeed, many efforts have been made to discover such a class of inhibitors, with some degree of success. 15,43,44 Compounds 1 and PM-56 displayed excellent SaeRS-related antivirulence activity in vitro and in vivo. These results were comparable to the effects seen in saeS knockout studies. Therefore, it was of great interest to determine whether these compounds inhibited the sensor HK SaeS. We tested the two compounds against the purified minimal kinase domain of SaeS. The minimal kinase domain contains a dimerization and histidine phosphotransfer (DHp) domain and a CA domain (amino acid residues 118−351). Measurements were conducted using a radioisotope method with SDS-PAGE autoradiography. Both compounds clearly inhibited the autophosphorylation of SaeS ( Figure 6), with IC 50 values of 220 and 160 μM for 1 and PM-56, respectively. This was generally consistent with their observed activities comparable to the saeS knockout. In contrast, when we measured the inhibition of phosphotransferase (PT) activity using radioactively phosphorylated SaeS in the presence of SaeR, no inhibition was observed ( Figure S8). This implies that only the HK activity (and not the PT activity) of SaeS is relevant to the inhibitory activity of 1 and PM-56.
To probe a possibility of 1 and PM-56 as broader HK inhibitors, we also tested both compounds on AgrC, a component of another master virulence regulator and the most well characterized TCS in S. aureus. 45 Thus, it was shown that 1 and PM-56 inhibited AgrC with IC 50 values of 339 and 140 μM, respectively ( Figure 6). PM-56 showed a comparable activity to that for SaeS, while 1 showed 1.5-fold lower activity compared to that for SaeS. This extra activity of PM-56 may explain slightly higher but nonsignificant improvement in the protection effect of PM-56 over the saeS knockout. Furthermore, PM-56 was 1.4-fold higher activity than 1 for SaeS, while this difference expanded to 2.4-fold for AgrC. Thus, this suggests a potential of the xanthoangelol B scaffold for further development of broad-spectrum or specific inhibitor of bacterial histidine kinases.

Journal of Medicinal Chemistry
Article Next, we attempted to measure the direct binding of 1 and PM-56 with SaeS using the intrinsic fluorescence quenching assay. Since SaeS contains seven Tyr (and zero Trp) residues, the Tyr fluorescence at 306 nm was monitored before and after binding of 1 and PM-56. The reduction of fluorescence upon ligand binding was expressed as the percent fluorescence quenching, and the K d value was calculated by nonlinear regression fitting ( Figure S9). The quenching assay revealed that 1 and PM-56 bound to SaeS with the K d values of 40 and 6.8 μM, respectively. These K d values are smaller than IC 50 values. It is not rare that IC 50 values are measured to be much higher than K d values, 46−48 and the data are clear indication that both compounds directly bind to SaeS with consistent trends of stronger interaction of SaeS with PM-56 than with 1.
Since the binding of PM-56 to SaeS was stronger than that of 1 as revealed in the inhibition and fluorescence quenching studies, we selected PM-56 for further dissection of the inhibitor binding to SaeS. To identify the binding of PM-56 to SaeS, one-dimensional transverse relaxation filtered experiment with a Carr−Purcell−Meiboom−Gill pulse trains (1D CPMG) and 1D saturation transfer difference experiment (1D STD) were performed in the presence and absence of the minimal kinase domain of SaeS. 49 The 1D CPMG experiment is able to detect the reduced T2-relaxation time of the PM-56 peaks caused by the interaction with SaeS, which results in the peak broadening and decreasing. The STD NMR spectra obtained with the minimal kinase domain of SaeS were virtually identical to those with the kinase domain of SaeS fused to maltose binding protein (MBP-SaeS) (Figure 7 and Figure S10), indicating that the presence of MBP does not influence the binding. Therefore, MBP-SaeS was used for interpretation of the 1D STD experiment to enhance the efficiency of saturation transfer from the protein to PM-56. The transferred saturation from the protein to the ligand is amplified by the binding exchange process and is efficient for target proteins with large molecular weight. 50 PM-56 clearly displayed the binding exchange process to SaeS. Simple 1D spectra also showed that the peaks of PM-56 were specifically broadened and shifted by SaeS, as compared to those of free PM-56 ( Figure 7A). Although the SaeS and MBP-SaeS proteins were dialyzed in the NMR-buffer before the NMR experiments, the HEPES molecule included in the purified proteins was not completely removed. Nevertheless, the spectra of the 1D CPMG ( Figure  7B) and 1D STD ( Figure 5C) clearly show that PM-56 specifically binds to SaeS, but HEPES did not. Furthermore, the similar peak intensities between the 1D spectrum ( Figure  7A, red spectrum) and the 1D STD spectrum ( Figure 7C) showed putative interactions involving most parts of PM-56. Hence, the entire molecular structure appears to be needed to exert a meaningful inhibition, which is consistent with the test of fragments. The presence of binding exchange process accompanied by the clear peak broadening of PM-56 indicates that the binding affinity between SaeS and PM-56 is likely not too strong (i.e., nanomolar K d ) or too weak (i.e., millimolar K d ), which is in agreement with inhibition and binding measurements.
The IC 50 values for HK activity were higher than expected considering the IC 50 values for the SaeRS TCS GFP reporter in intact bacteria cells. Nevertheless, an observation of substantially lower activity for purified enzymes than enzymes in intact cells is not rare. 51,52 This could be attributed to the different states of purified target proteins compared to proteins in intact cells. There are several factors that may affect protein activity in intact cells, such as the intracellular distribution of compounds, processing of target proteins, and presence of interacting proteins. 52 In our case, the purified SaeS comprised the minimal kinase domain, which contains only the DHp and CA domains (amino acid residues 118−351). However, native SaeS is a modular protein containing transmembrane domains, a linker, and cytoplasmic domains. The presence of the linker has a significant impact on the kinase activity. 53 Therefore, it is possible that integrity of the whole protein structure is crucial for its kinase activity. This would also affect its binding affinity to small molecules. Subsequently, the two inhibitors may appear to exert a lower inhibitory activity on the purified SaeS minimal kinase domain. Nevertheless, inhibition of the kinase activity of SaeS by 1 and PM-56, supported by STD NMR and the fluorescence quenching assay, is a significant indicator of their mechanism of action.

Article
We cannot rule out possible contributions to inhibition by other mechanisms, such as SaeS sensing or SaeR binding to the promoter regions of virulence genes. Although a majority of the inhibitors of TCSs reported thus far 54 have been HK inhibitors, a few have shown separate activities against the sensor 16 or the response regulator. 15 The evaluation of these possibilities is complicated, particularly for inhibitors of sensing activity because SaeS does not have a sensing domain and likely requires an unknown auxiliary protein. 53 Nevertheless, these studies are currently underway and will be reported in due course. Given that 1 and PM-56 did not suppress bacterial growth at IC 90 (while inhibiting the SaeRS TCS), these compounds are excellent candidates for specific action against the virulence of S. aureus. In light of this, the necessity of the whole xanthoangelol B molecule for optimal activity is an interesting finding. Neither the isoprenyl nor the chalcone unit alone displayed activity. Moreover, an overlapping fragment also failed to show activity. Compounds 1 and PM-56 are modular compounds composed of isoprene and chalcone. This result therefore deviates slightly from the logic of a fragmentbased approach. 55 This demonstrates that great caution is needed with fragment-based approaches (although these are still powerful methods of drug development). The major difference between 1 and PM-56 is the conversion of the α,βunsaturated carbonyl in the chalcone moiety of 1 to the βhydroxyl carbonyl in compound PM-56. This subtle change appears to confer a different activity profile. This will be valuable information in the further development of antivirulence agents. From the perspective of drug development, it may be beneficial to think about the route of administration. It was shown that orally administered xanthoangelol, a structurally related compound, was absorbed and distributed rapidly in mice in a single dose study, 56 which allows speculation that 1 and PM-56 may behave similarly. However, in the same study, the absorption efficiency of the chalcone was evaluated to be low, 56 potentially indicative of low oral bioavailability. The authors stated the limitation of a single dose study and the major difference between the results of bioavailability studies in animals and humans for possible reasons. Hence, this suggests that many aspects need to be taken into consideration, and it is considered that it is too early at the current stage to speculate the route of administration. Regardless, our data provide a strong starting point for further optimization.

■ CONCLUSION
In conclusion, we found a hit (xanthoangelol B 1) in a screening with inhibitory activity against the SaeRS TCS of S. aureus. A prenylated chalcone, xanthoangelol B 1, was synthesized in the racemic form and then resolved into an enantiomeric form. Synthesized xanthoangelol B 1 showed identical spectroscopic and optical properties to those of xanthoangelol B 1 extracted from a natural source. We also synthesized PM-56, a derivative of 1 with a hydrated chalcone double bond. The two compounds displayed similarly good inhibitory activities against the SaeRS TCS GFP reporter. They also inhibited the expression of downstream virulence factors, reducing hemolysis. PM-56 displayed a better tolerance for bacterial growth, which is desirable for antivirulence agents. Interestingly, fragments of the two compounds did not show any inhibitory activity. Thus, these compounds are required in their entirety for activity. Compounds 1 and PM-56 displayed excellent survival rates and low bacterial burdens in the G. mellonella infection model. These results were comparable to the effect of saeS knockout. The in vivo data demonstrated a good correlation with the in vitro data. Indeed, 1 and PM-56 showed substantial inhibition of the minimal kinase domain of SaeS, although measured IC 50 values were higher than expected from the in vitro (bacteria) and in vivo data. However, the purified minimal kinase domain is not likely to have the same kinase activity as the full-length membrane protein SaeS. This might explain the lower potency of 1 and PM-56 against the purified minimal SaeS. Fluorescence quenching assay and NMR data support the direct binding of PM-56 with the minimal kinase domain of SaeS. This study demonstrates that natural products are still a valuable source of compounds for therapeutic agents. Efforts toward the partial or total chemical synthesis or modification of natural products can help diversify a candidate's structure. With the expertise and insight gained from our synthesis of xanthoangelol B 1, we are now in a position to further develop inhibitors of the SaeRS TCS.

■ EXPERIMENTAL SECTION
General Methods. All reactions were carried out under open flask unless otherwise stated. THF, toluene, diethyl ether, and CH 2 Cl 2 were procured from Sigma-Aldrich. Reagents were purified prior to use unless otherwise stated. Purification of reaction products was carried out by flash chromatography using Fisher silica gel (35−70 mesh). NMR spectra were recorded on Bruker DPX 400. 1 H NMR spectra were measured at 400 and 500 MHz. 13 C NMR spectra were measured at 126 and 150 MHz using CDCl 3 , acetone-d 6 , or DMSO-d 6 as a solvent and internal reference. Coupling constants Js are given in Hz. Multiplicity as follows: s = singlet, d = doublet, t = triplet, q = quartet, m = multiplet, br = broad signal. Mass spectrometric data were obtained on a Waters Acquity UPLC and UPC 2 LC systems. Agilent 1260 infinity was used as an HPLC system to analyze and purify compounds. Synthesized xanthoangelol B 1 and PM-56 were screened for PAINS chemotypes using two online tools, PATTERNS (http://zinc15.docking.org/patterns/home) and FAFDrug4 (http:// fafdrugs3.mti.univ-paris-diderot.fr/), and the results were negative or PASS.

Journal of Medicinal Chemistry
Article Bu-4-oxa-5-azahomoadamantane (2.4 μmol) were dissolved in CH 2 Cl 2 (5 mL). After the reaction mixture was cooled to −40°C, trichloroisocyanuric acid (TCCA, 0.024 mmol) was added, and the mixture was stirred at a fixed temperature (−40°C) for 12 h. The mixture was diluted with i-PrOH (1 mL) followed by saturated aqueous NaHCO 3 (10 mL) and warmed to room temperature. The aqueous layer was separated and extracted with CHCl 3 . The combined organic layers were dried over MgSO 4 and concentrated under reduced pressure. The residue was purified by column chromatography (45/55 ethyl acetate/hexane, R f = 0.3) to give the ketone (48%) and the desired chiral product (34%). Spectroscopic data were identical as above.
[α] D 20 = +13.5 (MeOH, c = 0.5). Geraniolene Oxide (8). A solution of geraniolene (8.85 mmol) in CHCl 3 (30 mL) was cooled and stirred in an ice-bath to which a solution of 80% mCPBA (9.74 mmol) in ether (5 mL) was added dropwise over 30 min and stirred further for an additional 30 min. The reaction mixture was washed with 10% aqueous Na 2 CO 3 (4 × 10 mL) and saturated NaCl (10 mL). The organic layer was dried (anh. Mg 2 SO 4 ), and the solvents were removed on a rotary evaporator to yield a colorless liquid. The crude product was purified by short path distillation under vacuum (ca. 1 mmHg) at room temperature to yield 45% of geraniolene oxide Yield 45%, pale yellow oil: 1  Allyl Aryl Ether (9). 2,4-Dihydroxy acetophenone (6.57 mmol) was dissolved in dry acetone (10 mL), and both anhydrous K 2 CO 3 (9.85 mmol) and allyl bromide (7.23 mmol) were added. The mixture was refluxed and monitored by thin-layer chromatography (TLC). After completion of the reaction (8 h), the reaction mixture was filtered and acetone was removed under vacuum. The residue was purified by flash chromatography (9/1 ethyl acetate/hexane, R f = 0.4) on silica gel to yield the desired ally aryl ether in an average yield of 76%. 1  1-(3-Allyl-2,4-dihydroxyphenyl)ethan-1-one (10). The allyl aryl ether (9) (10.41 mmol) was dissolved in 20 mL of N,N-diethylaniline and refluxed at 220°C overnight. The reaction upon completion was cooled to room temperature and poured into 50 mL of precooled hexane. The separated solid was filtered and washed further with an excess amount of hexane. The product was purified by column chromatography (4/6 ethyl acetate/hexane, R f = 0.5).
Yield 60%, yellow amorphous powder, 1  2,6-Dimethylhepta-1,5-diene (12). A sealed tube was charged with molecular sieves (4 Å, 15 g), citral (32.84 mmol), and palladium acetate (10 mol %). The tube was then purged with argon several times. Under a counter flow of argon, remaining liquid reagents were added, followed by cyclohexane (10 mL) by syringe. The tube was tightly closed by screw cap and placed in a preheated oil bath at required temperature. The reaction mixture was vigorously stirred for 24 h. The reaction mixture was cooled to room temperature and filtered through Celite. Reaction tube and residue were washed with ethyl acetate (20 mL). The filtrate was concentrated, and the resulting deformylated product was purified via column chromatography using silica gel (1/99 ethyl acetate/petroleum ether, R f = 0.7).
Yield 80% yellow solid. 1  mCPBA Oxidation of Geranyl Bromide (18). Geranyl bromide (13.35 mmol) was dissolved in 30 mL of chloroform and cooled to 0°C . mCPBA (14.02 mmol) was added in small portions over 30 min. The reaction mixture was stirred further for 30 min at 0°C. After completion of the reaction, it was extracted five times with 1 M NaHCO 3 . Organic layer was washed with water, brine, dried over MgSO 4 , and concentrated to give the crude product, which was used further without purification.
Yield 67%, dark yellow solid. 1 13  HPLC Analysis for Purity. Kinetically resolved xanthoangelol B 1 and PM-56 were analyzed for their purity using an HPLC. The used system was the Agilent Technologies 1260 Infinity system with a C18 Column (Kromasil 100−5-C18, 5 μm, 21.2 × 250 mm). The column was eluted with 20/80 acetonitrile/water and the elution monitored with a UV detector at 254 nm. The program was run for 42 min for 1 and 25 min for PM-56. HPLC showed a single peak for each product, and the purity is judged to be over 95% ( Figure S11).
HPLC Analysis. For all other HPLC analyses, the same system was used under the same condition except the mobile phase (15/85 acetonitrile/water). The program running time was typically 25 min.

* S Supporting Information
The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.jmedchem.8b01012.