ACS Publications. Most Trusted. Most Cited. Most Read
DNA–Nanoparticle Composites Synergistically Enhance Organophosphate Hydrolase Enzymatic Activity

OR SEARCH CITATIONS

My Activity
Publications

Figure 1Loading Img
Download Hi-Res ImageDownload to MS-PowerPointCite This:ACS Appl. Nano Mater. 2018, 1, 7, 3091-3097
    Letter

    DNA–Nanoparticle Composites Synergistically Enhance Organophosphate Hydrolase Enzymatic Activity
    Click to copy article linkArticle link copied!

    • Anirban Samanta
      Anirban Samanta
      Center for Bio/Molecular Science and Engineering, Code 6900, United States Naval Research Laboratory, Washington, D.C. 20375, United States
      College of Science, George Mason University, Fairfax, Virginia 22030, United States
      More by Anirban Samanta
    • Joyce C. Breger
      Joyce C. Breger
      Center for Bio/Molecular Science and Engineering, Code 6900, United States Naval Research Laboratory, Washington, D.C. 20375, United States
      More by Joyce C. Breger
    • Kimihiro Susumu
      Kimihiro Susumu
      Optical Sciences Division, Code 5600, United States Naval Research Laboratory, Washington, D.C. 20375, United States
      KeyW Corporation, Hanover, Maryland 21076, United States
      More by Kimihiro Susumu
      Orcidhttp://orcid.org/0000-0003-4389-2574
    • Eunkeu Oh
      Eunkeu Oh
      Optical Sciences Division, Code 5600, United States Naval Research Laboratory, Washington, D.C. 20375, United States
      KeyW Corporation, Hanover, Maryland 21076, United States
      More by Eunkeu Oh
      Orcidhttp://orcid.org/0000-0003-1641-522X
    • Scott A. Walper
      Scott A. Walper
      Center for Bio/Molecular Science and Engineering, Code 6900, United States Naval Research Laboratory, Washington, D.C. 20375, United States
      More by Scott A. Walper
      Orcidhttp://orcid.org/0000-0002-9436-3456
    • Nabil Bassim
      Nabil Bassim
      Materials Science and Technology Division, Code 6300, United States Naval Research Laboratory, Washington, D.C. 20375, United States
      More by Nabil Bassim
    • Igor L. Medintz*
      Igor L. Medintz
      Center for Bio/Molecular Science and Engineering, Code 6900, United States Naval Research Laboratory, Washington, D.C. 20375, United States
      *E-mail: [email protected]
      More by Igor L. Medintz
      Orcidhttp://orcid.org/0000-0002-8902-4687
    Other Access OptionsSupporting Information (1)

    ACS Applied Nano Materials

    Cite this: ACS Appl. Nano Mater. 2018, 1, 7, 3091–3097
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acsanm.8b00933
    Published July 17, 2018
    Copyright © 2018 American Chemical Society
    Request reuse permissions

    Abstract

    Click to copy section linkSection link copied!
    Abstract Image

    Cell-free synthetic biology relies on optimally exploiting enzymatic activity, and recent demonstrations that nanoparticle (NP) and DNA scaffolding can enhance enzyme activity suggest new avenues toward this. A modular architecture consisting of a DNA cage displaying semiconductor quantum dots (QDs) that, in turn, ratiometrically display the organophosphate hydrolase phosphotriesterase (PTE) was utilized as a model system. Increasing DNA cage concentration relative to QD-PTE and creating a dense composite enhanced PTE rates up to 12.5-fold, suggesting strong synergy between the NP and DNA components; this putatively arises from increased enzymatic stability and alleviation of its rate-limiting step. Such bioinorganic composites may offer new scaffolding approaches for synthetic biology.

    Copyright © 2018 American Chemical Society

    Subjects

    what are subjects

    Keywords

    what are keywords

    Read this article

    To access this article, please review the available access options below.

    Get instant access

    Purchase Access

    Read this article for 48 hours. Check out below using your ACS ID or as a guest.

    Recommended

    Access through Your Institution

    You may have access to this article through your institution.

    Your institution does not have access to this content. Add or change your institution or let them know you’d like them to include access.

    Supporting Information

    Click to copy section linkSection link copied!

    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acsanm.8b00933.

    • Detailed experimental information and additional supporting data (PDF)

    Terms & Conditions

    Electronic Supporting Information files are available without a subscription to ACS Web Editions. The American Chemical Society holds a copyright ownership interest in any copyrightable Supporting Information. Files available from the ACS website may be downloaded for personal use only. Users are not otherwise permitted to reproduce, republish, redistribute, or sell any Supporting Information from the ACS website, either in whole or in part, in either machine-readable form or any other form without permission from the American Chemical Society. For permission to reproduce, republish and redistribute this material, requesters must process their own requests via the RightsLink permission system. Information about how to use the RightsLink permission system can be found at http://pubs.acs.org/page/copyright/permissions.html.

    Cited By

    Click to copy section linkSection link copied!
    Citation Statements
    Explore this article's citation statements on scite.ai
    powered by  Scite

    This article is cited by 17 publications.

    1. Kailin Feng, Jiayuan Alex Zhang, Wei-Ting Shen, Tianle Leng, Zhidong Zhou, Yiyan Yu, Weiwei Gao, Liangfang Zhang. Recent Development of Nanoparticle Platforms for Organophosphate Nerve Agent Detoxification. Langmuir 2025, 41 (4) , 2124-2140. https://doi.org/10.1021/acs.langmuir.4c04637
    2. Shelby Hooe, Joyce Breger, Scott Dean, Kimihiro Susumu, Eunkeu Oh, Scott Walper, Gregory A. Ellis, Igor L. Medintz. Benzaldehyde Lyase Kinetic Improvements, Potential Channeling to Alcohol Dehydrogenase, and Substrate Scope when Immobilized on Semiconductor Quantum Dots. ACS Applied Nano Materials 2022, 5 (8) , 10900-10911. https://doi.org/10.1021/acsanm.2c02196
    3. Sebastián A. Díaz, Priscilla Choo, Eunkeu Oh, Kimihiro Susumu, William P. Klein, Scott A. Walper, David A. Hastman, Teri W. Odom, Igor L. Medintz. Gold Nanoparticle Templating Increases the Catalytic Rate of an Amylase, Maltase, and Glucokinase Multienzyme Cascade through Substrate Channeling Independent of Surface Curvature. ACS Catalysis 2021, 11 (2) , 627-638. https://doi.org/10.1021/acscatal.0c03602
    4. Mônica A. Cotta (Associate Editor of ACS Applied Nano Materials). Quantum Dots and Their Applications: What Lies Ahead?. ACS Applied Nano Materials 2020, 3 (6) , 4920-4924. https://doi.org/10.1021/acsanm.0c01386
    5. Gregory A. Ellis, William P. Klein, Guillermo Lasarte-Aragonés, Meghna Thakur, Scott A. Walper, Igor L. Medintz. Artificial Multienzyme Scaffolds: Pursuing in Vitro Substrate Channeling with an Overview of Current Progress. ACS Catalysis 2019, 9 (12) , 10812-10869. https://doi.org/10.1021/acscatal.9b02413
    6. Mikael Madsen, Kurt V. Gothelf. Chemistries for DNA Nanotechnology. Chemical Reviews 2019, 119 (10) , 6384-6458. https://doi.org/10.1021/acs.chemrev.8b00570
    7. Tatiana Pashirova, Rym Salah-Tazdaït, Djaber Tazdaït, Patrick Masson. Applications of Microbial Organophosphate-Degrading Enzymes to Detoxification of Organophosphorous Compounds for Medical Countermeasures against Poisoning and Environmental Remediation. International Journal of Molecular Sciences 2024, 25 (14) , 7822. https://doi.org/10.3390/ijms25147822
    8. Shelby L. Hooe, Christopher M. Green, Kimihiro Susumu, Michael H. Stewart, Joyce C. Breger, Igor L. Medintz. Optimizing the conversion of phosphoenolpyruvate to lactate by enzymatic channeling with mixed nanoparticle display. Cell Reports Methods 2024, 4 (5) , 100764. https://doi.org/10.1016/j.crmeth.2024.100764
    9. Sandra Kröll, Teresa Burgahn, Kersten S. Rabe, Matthias Franzreb, Christof M. Niemeyer. Nano‐ and Microscale Confinements in DNA‐Scaffolded Enzyme Cascade Reactions. Small 2024, 20 (4) https://doi.org/10.1002/smll.202304578
    10. Himanshu Mali, Chandni Shah, B.H. Raghunandan, Anil S. Prajapati, Darshan H. Patel, Ujjval Trivedi, R.B. Subramanian. Organophosphate pesticides an emerging environmental contaminant: Pollution, toxicity, bioremediation progress, and remaining challenges. Journal of Environmental Sciences 2023, 127 , 234-250. https://doi.org/10.1016/j.jes.2022.04.023
    11. Elena Efremenko, Ilya Lyagin, Aysel Aslanli, Nikolay Stepanov, Olga Maslova, Olga Senko. Carrier Variety Used in Immobilization of His6-OPH Extends Its Application Areas. Polymers 2023, 15 (3) , 591. https://doi.org/10.3390/polym15030591
    12. Xiaoqi Wang, Zhenfu Wang, Linling Yu, Qinghong Shi, Xiaoyan Dong, Yan Sun. Zwitterionic polymer-mediated immobilization of organophosphorus hydrolase enhances hydrolysis of methyl parathion by substrate enrichment. Biochemical Engineering Journal 2022, 184 , 108491. https://doi.org/10.1016/j.bej.2022.108491
    13. Humaira Arshad, Abdul Majid, Muhammad Azmat Ullah Khan. Quantum Dots: Synthesis, Properties, and Applications. 2022, 11-45. https://doi.org/10.1007/978-3-031-10216-5_2
    14. Lijie Wang, Yan Sun. Engineering organophosphate hydrolase for enhanced biocatalytic performance: A review. Biochemical Engineering Journal 2021, 168 , 107945. https://doi.org/10.1016/j.bej.2021.107945
    15. Xuye Lang, Xiao Hong, Cetara A. Baker, Tamara C. Otto, Ian Wheeldon. Molecular binding scaffolds increase local substrate concentration enhancing the enzymatic hydrolysis of VX nerve agent. Biotechnology and Bioengineering 2020, 117 (7) , 1970-1978. https://doi.org/10.1002/bit.27346
    16. Meghna Thakur, Igor L. Medintz, Scott A. Walper. Enzymatic Bioremediation of Organophosphate Compounds—Progress and Remaining Challenges. Frontiers in Bioengineering and Biotechnology 2019, 7 https://doi.org/10.3389/fbioe.2019.00289
    17. Megan E. Muroski, Eunkeu Oh, JeffreyR. Deschamps, James B. Delehanty. Display of Potassium Channel–Blocking Tertiapin‐Q Peptides on Gold Nanoparticles Enhances Depolarization of Cellular Membrane Potential. Particle & Particle Systems Characterization 2019, 36 (3) https://doi.org/10.1002/ppsc.201800493
    Download PDF
    Go to ACS Applied Nano Materials
    Get e-Alerts
    Get e-Alerts

    ACS Applied Nano Materials

    Cite this: ACS Appl. Nano Mater. 2018, 1, 7, 3091–3097
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acsanm.8b00933
    Published July 17, 2018
    Copyright © 2018 American Chemical Society
    Request reuse permissions

    Article Views

    629

    Altmetric

    -

    Citations

    17
    Learn about these metrics

    Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.

    Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.

    The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated.