A Rational Active-Site Redesign Converts a Decarboxylase into a C=C Hydratase: “Tethered Acetate” Supports Enantioselective Hydration of 4-Hydroxystyrenes

The promiscuous regio- and stereoselective hydration of 4-hydroxystyrenes catalyzed by ferulic acid decarboxylase from Enterobacter sp. (FDC_Es) depends on bicarbonate bound in the active site, which serves as a proton relay activating a water molecule for nucleophilic attack on a quinone methide electrophile. This “cofactor” is crucial for achieving improved conversions and high stereoselectivities for (S)-configured benzylic alcohol products. Similar effects were observed with simple aliphatic carboxylic acids as additives. A rational redesign of the active site by replacing the bicarbonate or acetate “cofactor” with a newly introduced side-chain carboxylate from an adjacent amino acid yielded mutants that efficiently acted as C=C hydratases. A single-point mutation of valine 46 to glutamate or aspartate improved the hydration activity by 40% and boosted the stereoselectivity 39-fold in the absence of bicarbonate or acetate.

T he ability of an enzyme to catalyze a reaction other than its annotated "natural" activity is known as catalytic promiscuity 1−3 and is the result of evolutionary processes upon the encounter of "non-natural" substrates and the organism's striving for survival. 4−6 This phenomenon is an important criterion when selecting enzymes for the development of biocatalysts for the selective transformation of synthetic compounds, using rationally guided or randomly based directed evolution protocols. 7 Coumaric acids and their derivatives constitute monomeric units of lignin in plant cell walls 8 and are a major waste product of palm oil manufacturing. 9,10 In nature, their degradation is achieved by ferulic and phenolic acid decarboxylases (FDCs and PADs, respectively). These enzymes cleave their substrates into CO 2 and their respective 4hydroxystyrenes [1 (Scheme 1a)], which constitute undesired off-flavor components in beer and wine 11 but have also found application as renewable building blocks for polymers with interesting dielectric properties. 12−14 By supplying an excess of CO 2 in the form of bicarbonate, researchers can perform the process as the reverse β-carboxylation for the production of substituted coumaric acid derivatives (Scheme 1a). 15−17 During studies of regioselective carboxylation, the promiscuous hydration of 4-hydroxystyrene derivatives by FDCs and PADs in the presence of bicarbonate was discovered (Scheme 1b), and the best results were obtained with a ferulic acid decarboxylase from Enterobacter sp. (FDC_Es). 18 The nucleophile scope apart from water was extended to methoxy-amine, cyanide, and propanethiol, which are added via an analogous mechanism to furnish (S)-configured benzylic amines, nitriles, and thioethers, however without the need for bicarbonate (Scheme 1b). 19 The biocatalytic hydration of 4-hydroxystyrenes allows easy access to the (S)-1-(4-hydroxyphenyl)ethanol structural motif (2) from renewable resources in a stereoselective fashion without the need for protecting groups. The product hydrate is a substructure of bioactive molecules exerting herbicidal, 20 insecticidal, 21,22 antiproliferative, 23 and (hepatitis C) protease inhibition activities. 24,25 Though little is known about the active enantiomeric forms of these congeners, an improvement in biocatalytic styrene hydration would be of practical interest to facilitate the exploitation of the benzylic alcohols as chiral building blocks.
Experimental data 18 and quantum mechanical calculations 26 suggest that the promiscuous hydration of 4-hydroxystyrene requires a bicarbonate anion as a "cofactor", which is bound to Arg49 in the active site of the decarboxylase to achieve good conversion and a high enantiomeric excess (ee) of the benzylic alcohol product, (S)-2 (Scheme 2). Potassium bicarbonate (0.5 M, pH 8.5) promotes the hydration of 4-vinylphenol (1) with ∼65% conversion and ∼80% ee using FDC_Es as a biocatalyst. 18 In the absence of bicarbonate, however, a water molecule occupies its position, resulting in (almost complete) loss of stereoselectivity. 26 Consequently, supplementation of bicarbonate (>0.5 M) is required to maintain stereoselectivity, which renders this process economically and operationally less attractive.
In initial studies, the compensability of bicarbonate as a "cofactor" was investigated by replacing it with different (anionic) additives ( Figure 1). Potassium phosphate buffer (50 mM) was used as a reaction buffer, and the pH was adjusted to 8.0 after dissolving the additive salts to avoid bicarbonate decomposition (pK aH = 7.7) and to allow for direct comparison of additive effects. Control reactions in neat phosphate buffer gave moderate conversion (∼60%) but completely lacked stereoselectivity. Addition of Cl − , HPO 4 2− , or SO 4 2− improved the conversion slighty, while imidazole had adverse effects, again without stereoinduction. Borate gave a low ee (15%), while the conversion and ee were significantly improved by bicarbonate. Remarkably, the best values for conversion and ee were obtained in the presence of acetate. Next, the concentration of bicarbonate and acetate was varied within a range of 10−500 mM ( Figure S3). While the conversion profiles for both ions showed a similar behavior at increasing ion concentrations (leveling off at ∼90−95% conversion and 250 and 300 mM), the product ee profile

ACS Catalysis
Research Article with bicarbonate differs from that with acetate in a shallow maximum of 33% ee (at ∼100 mM), while the ee rises in a linear fashion with an increased acetate concentration, reaching an ee of 45% at 500 mM. Because the carboxylate moiety appears to be an ideal promoter for stereoselective hydration, a set of carboxylic acid sodium salts with varying chain lengths, substituents, and branching patterns was compared at neutral pH (7.0) (Figure 1b). Significant positive effects on the stereoselectivity were detected with all aliphatic carboxylates, depending on the side-chain size (Et > i Pr > t Bu ≈ Me ≈ H) with a maximum for propanoate (74% ee). Trifluoroacetate enhanced neither conversion nor the ee, because of its low pK a , which disables it to act as a catalytic acid in proton transfer (cf. Scheme 2). Because of the formation of an unidentified side product, only 60% of the starting material was recovered in the presence of formate.
On the basis of these promising results, we envisioned the design of mutants bearing a "tethered carboxylate" acting as a catalytic residue in the active site to mediate the proton transfer, 27 thereby waiving the need for an external bicarbonate/carboxylate "cofactor". The amino acid residues introduced should meet two crucial requirements. (i) The spatial arrangement in the active site needs to mimic bound bicarbonate, suggesting residues near the bicarbonate binding site as targets for mutagenesis, and (ii) residues must be chemically competent surrogates of bicarbonate/carboxylate to shuttle protons between the substrate Cβ atom and Glu72.
After detailed inspection of active-site residues of FDC_Es, Val46 located on a mobile loop in the vicinity to the bicarbonate binding site was selected for mutations. On the basis of their ability to act as a three-atom proton shuttle, Val46 was exchanged with Glu, Gln, Asp, His, and Arg ( Figure 2). The validity of this strategy was proven by the fact that the introduction of a carboxylate group at position 46 with aspartate (Val46Asp) or glutamate (Val46Glu) enhanced the stereoselectivity 39-fold and boosted the conversion by ∼40%. The slightly higher stereoselectivity (∼6%) for the Asp versus the Glu mutant may be explained by the tether that is one CH 2 unit shorter and pulls the water nucleophile farther out of the plane of symmetry toward the si face of the substrate ( Figure  3). Apparently, only a carboxylate moiety is an efficient bicarbonate/acetate mimic, because mutations to histidine, glutamine, and arginine were not beneficial (maximum 57% conversion for Val46Arg). However, despite its lower activity, the Val46His mutant showed slight stereoselectivity (up to 17% ee).
A homology model of the Val46Asp/Glu mutants reveals that the additional carboxylate groups are well positioned in the proximity of a water molecule (W1) to activate it as nucleophile within the hydrogen bond network of Tyr27, Glu72, and Arg49 ( Figure 3). Changing the latter to a glutamate residue (thereby inverting the positive charge at this position into a negative one) could yield some additional hydration activity (86% conversion) but yielded a racemic product. Tyr39 was suggested to dictate the (S)-stereoselectivity through steric repulsion between its aromatic ring and the Cβ atom of the quinone methide intermediate, which consequently presents its si face to the water nucleophile located above the plane

ACS Catalysis
Research Article (Scheme 2). 26 A double variant having both the beneficial Val46Glu mutation and a neutral Arg49Met mutation did not affect the conversion but showed a decrease in stereoselectivity compared to that of the single mutant. Removal of the hydrogen bond donor Arg49 weakens the bonding network and renders Tyr39 less rigid, which is responsible for the lower ee of the product. The Val46Glu mutant was also tested with other nucleophiles (methoxyamine and cyanide), 19 which gave improved stereoselectivities compared to that of the wild-type enzyme (Table S3).
The reaction conditions for the rationally designed "hydratases" were optimized in terms of pH, and effects of various organic co-solvents were examined ( Figure S5). Furthermore, the stereoselectivity of the process was found to increase with a decrease in temperature ( Figure S4), indicating a major contribution of the enthalpy difference (ΔΔH ⧧ ) to the free energy difference between the enantiomeric transition states (ΔΔG ⧧ ). 28,29 Finally, hydration of 1 was achieved with the Val46Glu and Val46Asp variants with a maximum turnover number of ∼220 and a product ee of ≤91% with 50 mM substrate loading at pH 6.0 and 25°C (Tables S1 and S2). The process was subsequently performed on a 100 mg scale with 0.8 mol % of FDC_Es V46E to afford 68 mg of (S)-hydrate product (60% yield) with a high enantiomer purity (96% ee after recrystallization) underpinning the usability of this reaction on a preparative scale.
In conclusion, prompted by the observation of bicarbonateand acetate-assisted asymmetric hydration of hydroxystyrenes catalyzed by ferulic acid decarboxylase, we rationally designed a hydratase from this decarboxylase through mutation of an Aspor Glu-carboxylate moiety into the active site, which efficiently functions as a proton shuttle. The mutants showed 40% higher activity and 39-fold improved stereoselectivity, which allowed preparative-scale transformations with turnover numbers of ≤220.

Notes
The authors declare no competing financial interest.

■ ACKNOWLEDGMENTS
The authors thank Georg Steinkellner for his advice with respect to structural biology and Fahmi Himo for fruitful discussions about the reaction mechanism and thermodynamics. Funding by the Austrian Science Fund (FWF Project P26863) and the Austrian BMWFW, BMVIT, SFG, Standortagentur Tirol, Government of Lower Austria, and ZIT through the Austrian FFG-COMET-Funding Program is gratefully acknowledged.