Waterproof Cellulose-Based Substrates for In-Drop Plasmonic Colorimetric Sensing of Volatiles: Application to Acid-Labile Sulfide Determination in Waters

The present work reports on the assessment of widely available waterproof cellulose-based substrates for the development of sensitive in-drop plasmonic sensing approaches. The applicability of three inexpensive substrates, namely, Whatman 1PS, polyethylene-coated filter paper, and tracing paper, as holders for microvolumes of colloidal solutions was evaluated. Waterproof cellulose-based substrates demonstrated to be highly convenient platforms for analytical purposes, as they enabled in situ generation of volatiles and syringeless drop exposure unlike conventional single-drop microextraction approaches and can behave as sample compartments for smartphone-based colorimetric sensing in an integrated way. Remarkably, large drop volumes (≥20 μL) of colloidal solutions can be employed for enrichment processes when using Whatman 1PS as holder. In addition, the stability and potential applicability of spherical, rod-shaped, and core–shell metallic NPs onto waterproof cellulose-based substrates was evaluated. In particular, Au@AgNPs showed potential for the colorimetric detection of in situ generated H2S, I2, and Br2, whereas AuNRs hold promise for I2, Br2, and Hg0 colorimetric sensing. As a proof of concept, a smartphone-based colorimetric assay for determination of acid-labile sulfide in environmental water samples was developed with the proposed approach taking advantage of the ability of Au@AgNPs for H2S sensing. The assay showed a limit of detection of 0.46 μM and a repeatability of 4.4% (N = 8), yielding satisfactory recoveries (91–107%) when applied to the analysis of environmental waters.

T he development of analytical strategies enabling a straightforward, selective, and sensitive determination of target compounds by means of plasmonic nanoparticles (NPs) is a challenging task that can be tackled by the combination of miniaturized analytical approaches. 1−3 Of particular interest is the implementation of NPs in headspace microseparation approaches, since they potentially provide excellent enrichment factors with reduced matrix effects, while avoiding compatibility issues of NPs with complex samples. In this sense, a number of approaches have been reported in the literature to perform in-drop extraction of volatiles with simultaneous or sequential colorimetric detection. Downsized extraction approaches combined with miniaturized UV−vis spectrophotometers 2,4−6 and probes 7,8 have demonstrated their convenience for determination of target analytes at trace levels. More recently, nonconventional detection devices such as digital cameras and smartphones have received much interest for on-site analysis, mainly in combination with paperbased analytical devices (PADs). 9−13 These systems proved suitable when combined with both drops and cellulose substrates as platforms for enrichment of volatiles with subsequent colorimetric detection. 14−17 However, the use of PADs modified with plasmonic NPs for analyte sensing suffers from certain limitations, including potential aggregation and nonuniform distribution issues, as well as a reduced retention efficiency of NPs on cellulose substrates. 18 While certain NPs are conveniently retained in PADs by drop casting, 19 the retention of NPs at the surface of cellulose substrates is commonly limited, leading to faintly colored detection areas in PADs 20 that can affect their sensitivity when applied for colorimetric sensing. This drawback is usually minimized by immersion of cellulose substrates in colloidal solutions for extended exposure times or via vacuum filtration, 21 in spite of the relatively large amounts of colloidal solutions commonly required. NP-modified PADs can also be obtained by the dropcasting/heating approach 22 or by in situ formation of NPs in the detection areas of PADs. 22 The in situ synthesis of certain NPs with controlled sizes and morphologies in cellulose substrates is however a challenging task. Alternatively, colloidal solutions have been employed as extractant phases in singledrop microextraction (SDME), enabling the enrichment of volatiles with a reduced consumption of colloidal solution, typically in the range of 1−5 μL. However, the deposition of enriched microdrops on hydrophilic cellulose substrates for acquisition of the analytical signal results in a loss of sensitivity due to the aforementioned reduced retention of NPs on these materials. A remarkable and straightforward alternative involves the use of Eppendorf tubes for exposure of a drop of colloidal solution hanging from the cap with subsequent digitization for colorimetric analysis. 23 This approach, however, hinders the in situ generation of volatiles from nonvolatile analytes without losses of gaseous derivatives, and convection is not produced during extraction, thus potentially leading to extended extraction times and/or limited sensitivity. Solving the aforementioned limitations, while integrating steps, would be therefore highly desirable.
Cellulose substrates display excellent properties such as capillary-driven fluid transport, flexibility, and low cost that make them ideal for a broad range of analytical applications. 13,24−26 However, as discussed above, the hydrophilic nature and porosity of cellulose substrates hampers their applicability with colloidal solutions. 26 Hydrophobic cellulose substrates have been reported to overcome these drawbacks, enabling concentration of NPs in a small area. This strategy results in enhanced sensitivity and reproducibility, being exploited mainly in SERS analysis. 18,26,27 In addition, some hydrophobic and super-omniphobic materials have demonstrated their potential usefulness for the development of droplet-based optical assays with enhanced sensitivity due to the increased droplet height (i.e., path length). 28−30 In light of the above, cellulose substrates with significant water resistance could be particularly attractive as drop holders for both in-drop enrichment and plasmonic sensing purposes. To be applicable, solid substrates must be compatible with the microdrop of colloidal solution and display enough solid−liquid adhesion to prevent hanging drops from rolling off from the substrate. The latter requirement limits the use of super-omniphobic paper substrates with this aim, which nevertheless show much promise for colorimetric sensing. 30 To the best of our knowledge, this is the first work in which waterproof cellulose-based substrates are assessed as feasible surfaces to perform in-drop enrichment and colorimetric plasmonic sensing while integrating the different unitary steps.
Particularly, in situ generation of volatiles and extraction and preconcentration of evolved volatiles onto a microvolume of metallic NPs can be simultaneously performed, whereas the acquisition of the analytical signal can be rapidly carried out in the substrate itself without the need to collect and transfer the enriched drop. As a proof of concept, the proposed approach has been applied to the determination of acid-labile sulfide in waters.
Apparatus. A Samsung Galaxy A70 smartphone (Samsung, Seoul, South Korea) and a portable PULUZ photo studio lightbox (Shenzhen PULUZ Technology Limited, Shenzhen, China) equipped with 20 LEDs were used for digitization of microdrops of colloidal solutions. RGB Color Detector App (The Programmer, Google Play Store) and ImageJ, a free image processing and analysis software, 32 were used for data acquisition. The statistical package Statgraphics Centurion XVI.I (StatPoint Technologies, Warrengton, VA, USA) was employed for optimization purposes. With the aim of making comparative studies, a Xerox ColorQube 8580 printer (Rochester, New York, USA) and a Phoenix instrument RSM-02HP+ magnetic stirring hotplate (Garbsen, Germany) were also employed to define detection areas on hydrophilic cellulose substrates by wax-printing. 33 A Thermo Scientific Nanodrop ND-1000 microvolume UV−vis spectrophotometer (Wilmington, DE, USA) and a JEOL JSM-1010 (Tokyo, Japan) transmission electronic microscope with an acceleration voltage of 100 kV were used to characterize NPs in the absence and presence of volatile derivatives of target analytes. Drops of colloidal solutions were deposited onto carbon-coated copper grids and air-dried before TEM analysis.
Preparation of Colloidal Solutions. Spherical AuNPs, rodshaped AuNPs, and Au@Ag core−shell NPs have been prepared in accordance with synthetic procedures reported in the literature. 34−38 Detailed procedures are provided in the Supporting Material.
Experimental Procedure for Acid-Labile Sulfide Determination. 10 mL of aqueous solution (i.e., blank, standard or aqueous sample) was placed into a glass vial containing a stir bar. A 2.0-cmdiameter circular piece of a waterproof cellulose-based substrate, typically Whatman 1PS, was placed inside a screw cap over a Teflonfaced septum, and 10 μL of Au@AgNPs was deposited onto the water-resistant cellulose substrate before sealing the vial. Then, H 2 S was generated in situ by externally injecting 1.0 mL of HCl 1.0 M, and the hanging microdrop was exposed to the headspace above the aqueous solution stirred at 1200 rpm for a prescribed time. Finally, a smartphone was used for digitization of the microdrop and the analytical response (mean color intensity difference (ΔI c ) in the B color channel, calculated by subtracting the mean color intensity of a blank to the mean color intensity of a standard or sample) was obtained.
■ RESULTS AND DISCUSSION Assessment of NPs Stability on Waterproof Cellulose-Based Substrates. The stability of different colloidal solutions on waterproof cellulose-based substrates was first assessed. The potential applicability of NPs as receptors for volatile sensing depends largely on their stability on the cellulose-based substrates used as drop holders. With the timedependent nature of microextraction processes kept in mind, results derived from these studies are of paramount relevance. Thus, 5 μL of three different types of plasmonic NPs, namely, spherical AuNPs, gold nanorods (AuNRs), and Au@AgNPs, were deposited onto three inexpensive substrates, namely, Whatman 1PS, tracing paper, and polyethylene-coated filter paper. Images of the microdrops of colloidal solutions were obtained at regular intervals in the range of 0−30 min and analyzed by ImageJ. Figures S1−3 show the results derived from these studies. The stability of microdrops of colloidal solutions deposited onto cellulose-based substrates was highly dependent on the types of nanomaterials and substrates considered. In general terms, both AuNRs and Au@AgNPs showed a remarkable stability in the studied time interval, whereas the color changes produced within relatively short deposition times reflected the lack of stability of citrate-capped AuNPs, presumably due to ligand displacement and aggregation of AuNPs. Regarding the evaluated substrates, both Whatman 1PS and polyethylene-coated filter paper proved suitable for in-drop plasmonic sensing, since the mean color intensity of NPs in RGB channels remained steady. However, the tracing paper was found to be unsuitable with either colloidal solution attempted. The discoloration of microdrops deposited onto tracing paper can be ascribed to the increased interactions of NPs with this cellulose substrate that would lead to adsorption and presumably aggregation of NPs on the substrate.
The use of drop volumes larger than those typically used in SDME (i.e., 1−5 μL) is of particular interest in the development of in-drop enrichment/sensing assays. On one hand, larger drop volumes provide larger surface areas that can lead to extraction of higher amounts of volatiles, while at the same time, longer optical path lengths are obtained, 28 which leads to increased sensitivity. The potential spreading of colloidal solutions hanging from suitable waterproof substrates, namely, Whatman 1PS and polyethylene-coated filter paper, was then assessed. Particularly, drop volumes of AuNPs, AuNRs, and Au@AgNPs of up to 30 μL were hanged from the waterproof substrates in agreement with the procedure described in the Experimental Section, and the resulting drop diameter (D) was compared with the diameter of an analogous microdrop deposited at the same time onto the corresponding substrates (D 0 ). In general, Whatman 1PS enabled the exposure of larger drop volumes than polyethylene-coated filter paper and led to significantly lower D/D 0 values when drop volumes beyond 10 μL were attempted ( Figure S4). Remarkably, drops of colloidal solutions kept a quasi-spherical shape and displayed remarkable stability when Whatman 1PS substrates were used. As can be deduced from Figure S4, NP drop volumes of even 30 μL were found to be suitable for microextraction processes with the exception of AuNRs, which led to a significant rate of drop detachment when NP volumes beyond 20 μL were used. These effects can be attributed to the lower surface tension of CTAB-containing AuNRs drops. Remarkably, the increase in drop diameter of colloidal solutions was found to be lower than 20% in any case, regardless of the colloidal solutions considered and the substrates used. Furthermore, a good repeatability (RSD values below 2.0%) was observed in all cases when measuring the diameter of colloidal drops after the microextraction process.
The effect of drop volume (and hence, drop height) over the analytical response was also evaluated. This study was conducted by drop-casting colloidal solution volumes in the range of 2−30 μL onto Whatman 1PS. This substrate was selected on the basis of its compatibility with the colloidal solutions and its higher hydrophobicity (water contact angle of ca. 138°), 39 which ensured longer drop heights and, as a consequence, reduced deviations from the ideal spherical configuration. As shown in Figure S5, the analytical signal, i.e., mean color intensity in the corresponding RGB channel, significantly increased upon increasing drop volume, and a quasi-linear response was observed between the analytical signal and the theoretical drop height (as an estimation of the optical path length) assuming a perfectly spherical configuration, i.e., H = 2 × (3V/4π) 1/3 , where H is the theoretical drop height and V corresponds to the drop volume. 28 It is worth mentioning that larger volumes (e.g., 50 μL) of colloidal solutions showed significant stability when deposited onto Whatman 1PS or polyethylene-coated filter paper, thus being potentially applicable as cost-effective substrates for smartphone-based detection purposes. According to these results, Au@AgNPs and AuNRs were selected as the potential nanomaterials for sensing, whereas the hydrophobic Whatman 1PS was selected as the substrate of choice.
Sensing of Volatiles. Screening of target analytes was subsequently carried out to evaluate the potential applicability of the proposed approach. Different environmentally relevant analytes, i.e., bromide, iodide, sulfide, sulfite, nitrite, ammonia, methylamine, dimethylamine, trimethylamine, arsenic(III), antimony(III), and mercury(II), were tested. The process, schematically represented in Figure S6A, involved in situ generation of volatile derivatives, their transfer to the headspace and trapping onto a microdrop of colloidal solution with subsequent smartphone-based colorimetric detection. Accordingly, a microvolume of Au@AgNPs or AuNRs hanging from the hydrophobic cellulose substrate (Whatman 1PS) was exposed to volatiles generated in situ as described elsewhere (Table S1). 6,40−45 The digital images of AuNRs and Au@ AgNPs drops before and after exposure to the volatiles, as well as the corresponding UV−vis spectra and the analytical response (mean color intensity difference in each RGB channel), are shown in Figures 1 and 2. Obtained results and possible sensing mechanisms are discussed in the sections below.
Sensing Studies with AuNRs. Three of the evaluated volatile analyte derivatives, i.e., Br 2 , I 2 , and Hg 0 , yielded significant effects on the color of AuNRs-containing drops that could be exploited for sensing purposes (Figure 1). In particular, an apparent color change from blue-gray to yellow was produced when AuNRs were exposed to Br 2 . This effect can be ascribed to the oxidation of Au 0 with formation of AuBr 2 − in the drop. 4,46,47 Apart from a blue-shift and decrease in intensity of the longitudinal plasmonic band of AuNRs, the UV−vis spectra shows the appearance of the characteristic absorption peak of Br 3 − (272 nm), which acts as both an oxidizing and complexing agent, together with additional bands that could be attributed to the formation of the aforementioned gold bromide complexes. Less noticeable, even though significant, color changes associated with blue-shifts of the longitudinal plasmonic band of AuNRs occurred in the presence of I 2 and Hg 0 that can be ascribed to oxidative etching 6,48 and amalgamation, 5,49 respectively. TEM images of AuNRs in the absence and presence of Br 2 , I 2 , and Hg 0 are also provided in Figure 3. The obtained TEM images reinforced the assumption of oxidative etching and amalgamation as recognizing mechanisms for Br 2 , I 2 , and Hg 0 . As can be derived from the figure, the aspect ratio (AR) of AuNRs (2.6 ± 0.4) was significantly reduced when exposed to I 2 (1.5 ± 0.3), whereas less noticeable effects were produced in the presence of Br 2 (1.7 ± 0.4) and, specifically, Hg 0 (2.4 ± 0.5). With the results shown in Figure 1B kept in mind, colorimetric sensing of Br 2 , I 2 , and Hg 0 can be presumably carried out with AuNRs.  Thus, a ratiometric sensing approach involving R and B channels could be established for Br 2 sensing, the B channel could be employed for detection of Hg 0 , and all three channels could be employed for I 2 sensing even though with lower sensitivity ( Figure S6B).
Sensing Studies with Au@AgNPs. Results shown in Figure  2 point out that three of the evolved volatiles, namely, H 2 S, I 2 , and Br 2 , gave rise to a significant effect on the analytical response when Au@AgNPs were used. Particularly, the exposure of Au@AgNPs to H 2 S led to a decrease, widening, and red-shift of the plasmonic band with a color change from yellow to brown under the experimental conditions used in the study ( Figure 2C). Besides, Au@AgNPs droplets lost their yellow color when exposed to I 2 and Br 2 (Figure 2A and C). TEM images of Au@AgNPs in the absence and presence of these three volatiles are shown in Figure 4. No differences in morphology or in mean diameter (46 ± 11 nm vs 46 ± 14 nm) could be observed when Au@AgNPs were exposed to H 2 S ( Figure 4B). Nevertheless, a slight variation in polydispersity (ca. 25% increase) occurred after exposure of Au@AgNPs to the volatiles. The effect of H 2 S could be attributed to the formation of Ag 2 S, as described in the literature. 23,50,51 Unlike H 2 S, significant morphological changes of Au@AgNPs were observed when exposed to Br 2 and I 2 ( Figure 4C and D). Particularly, both halogens led to the formation of irregular structures probably derived from the partial oxidation of the metallic NPs, adsorption of formed halides over the surface of NPs, and fusion with the formation of metallic halides. 52−54 Regarding smartphone-based detection, the B channel enabled the detection of both bromide and iodide with higher sensitivity, whereas the highest analytical response for sulfide detection was achieved when using the G channel under the experimental conditions used in the study ( Figure 2B and S6C). These results suggest that Au@AgNPs could potentially be used for H 2 S, Br 2 , and I 2 colorimetric sensing by the proposed approach. As a proof of concept, the smartphonebased colorimetric determination of acid-labile sulfide in environmental waters was assessed in this work.
Assessment of Experimental Parameters for In-Drop Colorimetric Sensing of H 2 S. The effect of experimental variables on both in-drop trapping of H 2 S by Au@AgNPs and smartphone-based colorimetric detection was studied. In particular, the amount of formaldehyde employed for the preparation of Au@AgNPs of increasing size in accordance with the Tollens' reaction was first evaluated. In addition, digitization conditions, i.e., ISO and exposure value (EV), and two relevant microextraction parameters, i.e. microextraction time and drop volume, were evaluated for optimal response.
The amount of formaldehyde needed to prepare Au@ AgNPs in accordance with the Tollens' reaction was first evaluated. The use of increasing amounts of formaldehyde in the presence of spherical AuNPs and Tollens' reagent leads to the formation of Ag shells with increased thickness, 37 which could have a paramount effect on the analytical response. Thus, Au@AgNPs with increasing dimensional sizes were prepared, and the obtained colloidal solutions were evaluated for the enrichment and sensing of H 2 S. As can be observed in Figure 5, the highest analytical response was achieved when the volume of formaldehyde solution employed for preparing Au@ AgNPs was set at 125 μL, and consequently, these conditions were selected for further studies. It should be highlighted that the experimental conditions selected for the preparation of Au@AgNPs affected not only the sensitivity of the assay for sulfide determination but also the appropriate RGB color channel for data acquisition.
Digitization conditions were subsequently evaluated. ISO sensitivity, aperture, and shutter speed values define the amount of light reaching the camera sensor, and therefore, their assessment can be of paramount importance to ensure high sensitivity for analyte detection. The ISO setting enables the adjustment of the sensor's sensitivity to light. The higher the ISO, the higher the sensitivity to light. Besides, the EV is a combination of shutter speed and f-number, which defines the size of a lens aperture. As can be observed in Figure 6, the analytical response, ΔI c , was highly dependent on the EV and, to a lesser extent, on the ISO sensitivity. Remarkably, an appropriate selection of digitization conditions enabled a 5.5fold enhancement of the analytical response. The EV and ISO were set at +2.0 and 200, since these conditions ensured the highest sensitivity.  As described in the section Assessment of NPs Stability on Waterproof Cellulose-Based Substrates, pendant droplets of 20 μL can be easily employed for in-drop enrichment/sensing with hydrophobic cellulose substrates such as Whatman 1PS, unlike those exposed to the tip of a capillary or a syringe needle, which is standard practice in SDME. Increased droplet volumes could favorably affect not only the smartphone-based detection step as a result of the increased path length, but also the extractability of the volatile, due to the increased interfacial area of colloidal microdrops. However, extended exposure times could be required when increasing the droplet radius, 55 and partial dilution of extracted volatiles can occur, thus leading to lower enrichment factors. Thus, the combined effect of microextraction time and drop volume on the analytical response was evaluated for optimal performance. A central composite design (CCD) was selected with this aim. The levels of the experimental parameters, as well as the matrix of the CCD, are given in Table S2. As can be observed in the Pareto chart ( Figure S7), both experimental parameters as well as the interaction between both variables (denoted as AB in Figure S7) and the quadratic effects (denoted as AA and BB, respectively, in Figure S7) were found to be statistically significant at the 95% confidence level. An excellent relationship between experimental data and the fitted model was obtained, with R 2 = 0.99. As can be deduced from the response surface shown in Figure 7, the highest analytical response was achieved when using the longest microextraction times attempted (ca. 30 min) and drop volumes slightly above the central level (e.g., 12 μL). Thus, the possibility of using drop volumes greater than those typically used in SDME (i.e., 1−5 μL) proved to be convenient for in-drop trapping/sensing of H 2 S. The effect of Au@AgNPs drop volume on the analytical signal was highly dependent on the microextraction time used (Figure 7). Thus, the analytical response was largely dependent on the Au@AgNPs drop volume at reduced microextraction times, whereas the response was much less affected by the drop volume used when equilibrium conditions were reached. A microextraction time of 22 min and a drop volume of 10 μL were finally selected as a compromise between sensitivity and sample throughput.
Comparison with Alternative Approaches Involving Hydrophilic Cellulose Substrates. The proposed approach was compared with alternative strategies involving hydrophilic substrates for extraction and/or smartphone-based detection ( Figure S8). A hydrophilic substrate commonly used for the preparation of PADs, i.e., Whatman 1, was employed with this aim. For comparison purposes, studies were carried out with 2.0-cm-diameter circular pieces of both neat Whatman No. 1 and wax-printed Whatman No. 1 substrates with circular hydrophobic barriers defining a detection area with a diameter equivalent to the one of a drop of 10 μL of Ag@AuNPs deposited onto the hydrophobic substrate (i.e., ca. 0.27 cm). Hydrophobic barriers were fabricated as described elsewhere. 33 First, 2.0-cm-diameter circular pieces of both hydrophilic substrates were modified with a microvolume of Au@AgNPs and employed for both extraction and smartphone-based detection of sulfide. Alternatively, microdrops of Au@AgNPs enriched with H 2 S following the experimental procedure described in the Experimental Section were deposited on Whatman No. 1 substrates (with and without hydrophobic barriers) for digitization and analytical response acquisition. As can be noted in Figure S8, the analytical response achieved with the proposed approach was found to be substantially higher than that obtained using neat Whatman 1 substrates (7.5-to 7.8-fold) and higher than that obtained with waxprinted Whatman 1 substrates (1.4-to 1.7-fold), regardless of whether they were used for both extraction and nonconventional colorimetric detection or for smartphone-based detection only. Thus, the use of Au@AgNPs with Whatman 1PS proved advantageous in terms of sensitivity and simplicity for the smartphone-based determination of sulfide.
Analytical Performance. Under optimal conditions, the analytical performance for sulfide determination was evaluated. As can be observed in Figure 8A, the analytical response increased linearly with the concentration of the anion in the range of 1.6−13.0 μM, in agreement with the decrease of the characteristic peak of silver shell observed in the UV−vis spectra of Au@AgNPs drops exposed to increasing concentrations of H 2 S ( Figure 8B).
The limits of detection (LOD) and quantification (LOQ), calculated in accordance with the 3σ criterion, were found to be 0.46 μM and 1.5 μM, respectively. The repeatability, expressed as relative standard deviation (RSD), was 4.4% (N = 8). A comparison of analytical characteristics of the approach

ACS Sensors
pubs.acs.org/acssensors Article with previously described methods for H 2 S colorimetric sensing involving IT equipment is provided in Table 1.
A number of PADs and μPADs have been reported for H 2 S sensing, mainly based on the methylene blue formation at the detection area by reaction with the DMPD reagent, 56−58 but also on the CuS formation by reaction with a Cu(II) salt. 41 In addition, the potential of nanomaterials for H 2 S sensing, both incorporated into agarose gels and liquid phases, 23,59 has been assessed in some recent contributions. The reported assay showed excellent sensitivity and precision when compared with the aforementioned alternative colorimetric methods. Notably, the LOD achieved with the proposed system was comparable to or better than that displayed by other approaches involving enrichment in both liquid drops and solid substrates, and at least 10 times better than that of DMPD-based colorimetric systems. Furthermore, the proposed approach enables exposing relatively large drop volumes to the headspace with remarkable stability, performing in situ H 2 S generation without loses of the volatiles, and straightforward digitization and analysis of the microdrop. In addition, modification of the assessed waterproof substrates is not required for optimal performance, unlike in other approaches involving hydrophilic substrates. In this sense, functionalization of chromatographic paper with 3-aminopropyltriethoxysilane (APTES) followed by modification with the sensing materials was required to minimize the heterogeneity of the colored product upon reaction with H 2 S, 56 whereas hydrophobic barriers were commonly required to fabricate PADs and μPADs. 41,58 The applicability of the proposed method for determination of acid-labile sulfide in the environmental waters method was finally assessed, with analytical results shown in Table 2. Recoveries in the range of 91−107% were obtained in all cases, thereby showing the nonsignificant effect of matrix constituents on the analytical results.

■ CONCLUSIONS
In this work, the feasibility of waterproof cellulose-based substrates for the in-drop plasmonic sensing of volatiles with IT equipment was assessed. The novel approach encompasses the following features: (i) hydrophobic substrates demonstrate substantial compatibility with colloidal solutions and are particularly advantageous as drop holders, enabling the exposure of relatively large drop volumes (ca. 30 μL) for volatile enrichment with a negligible risk of detachment; (ii) the use of hydrophobic substrates enables the integration of unitary steps (i.e., in situ volatile generation, enrichment and   interaction of the volatile with the NPs, and image acquisition), thus simplifying the process; (iii) hydrophobic substrates behave as cuvetteless drop holders during image acquisition and ensure high sensitivity thanks to the increased path length; (iv) fabrication of PADs, commonly consisting of the formation of hydrophobic barriers for defining hydrophilic detection areas, is not required, and so, the sensitivity achieved with the drop-based approach is significantly larger than that obtained with PADs. Overall, the proposed approach was demonstrated to be straightforward and highly convenient for optical sensing purposes, and further developments based on the combination of a wide range of waterproof cellulose-based substrates with optically responsive materials for in-drop enrichment and sensing purposes can be anticipated.