Analytical chemistry
Biological and Medicinal Chemistry
This webinar will discuss the limitations of Ion Pairing Reversed Phase (IP-RP) chromatography and explore Hydrophilic Interaction Chromatography as an alternative, along with ion pairing-free RP approaches for oligonucleotide analysis using LC/MS.
This webinar will delve into the limitations of traditional Ion Pairing Reversed Phase (IP-RP) chromatography, particularly its high costs, environmental impact, and need for specialized equipment. We will explore Hydrophilic Interaction Chromatography (HILIC) as a promising alternative, highlighting its compatibility with mass spectrometry (MS) and the absence of ion-pairing reagents, which offers greater flexibility in instrumentation.
Additionally, we’ll discuss recent advancements in ion pairing-free RP approaches for oligonucleotide analysis using LC/MS, showcasing studies on a range of oligonucleotides from 14mer to 103mer. Key parameters for optimizing LC/MS performance in these methods will also be covered.
Speakers
Global Product Manager HPLC Bio Columns, Agilent Technologies
Application Scientist, Agilent Technologies