Novel Double-Layer Dissolving Microneedles for Transmucosal Sequential Delivery of Multiple Drugs in the Treatment of Oral Mucosa DiseasesClick to copy article linkArticle link copied!
- Yang MengYang MengState Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Research Unit of Oral Carcinogenesis and Management, Chinese Academy of Medical Sciences, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. ChinaMore by Yang Meng
- Xin Jiao LiXin Jiao LiSchool of Chemical Engineering, Sichuan University, Chengdu, Sichuan 610065, P.R. ChinaMore by Xin Jiao Li
- Yao LiYao LiSchool of Chemical Engineering, Sichuan University, Chengdu, Sichuan 610065, P.R. ChinaMore by Yao Li
- Tian Yu ZhangTian Yu ZhangState Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Research Unit of Oral Carcinogenesis and Management, Chinese Academy of Medical Sciences, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. ChinaMore by Tian Yu Zhang
- Dan LiuDan LiuState Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Research Unit of Oral Carcinogenesis and Management, Chinese Academy of Medical Sciences, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. ChinaMore by Dan Liu
- Yu Qi WuYu Qi WuState Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Research Unit of Oral Carcinogenesis and Management, Chinese Academy of Medical Sciences, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. ChinaMore by Yu Qi Wu
- Fei Fei HouFei Fei HouState Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Research Unit of Oral Carcinogenesis and Management, Chinese Academy of Medical Sciences, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. ChinaMore by Fei Fei Hou
- Lu YeLu YeState Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Research Unit of Oral Carcinogenesis and Management, Chinese Academy of Medical Sciences, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. ChinaMore by Lu Ye
- Chuan Ji WuChuan Ji WuState Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Research Unit of Oral Carcinogenesis and Management, Chinese Academy of Medical Sciences, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. ChinaMore by Chuan Ji Wu
- Xiao Dong FengXiao Dong FengState Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Research Unit of Oral Carcinogenesis and Management, Chinese Academy of Medical Sciences, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. ChinaMore by Xiao Dong Feng
- Xiao Jie Ju*Xiao Jie Ju*Email: [email protected]School of Chemical Engineering, Sichuan University, Chengdu, Sichuan 610065, P.R. ChinaState Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu, Sichuan 610065, P.R. ChinaMore by Xiao Jie Ju
- Lu Jiang*Lu Jiang*Email: [email protected]State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Research Unit of Oral Carcinogenesis and Management, Chinese Academy of Medical Sciences, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. ChinaMore by Lu Jiang
Abstract
![Abstract Image](/cms/10.1021/acsami.2c19913/asset/images/medium/am2c19913_0011.gif)
The development of transmucosal drug delivery systems is a practical requirement in oral clinical practice, and controlled sequential delivery of multiple drugs is usually required. On the basis of the previous successful construction of monolayer microneedles (MNs) for transmucosal drug delivery, we designed transmucosal double-layer sequential dissolving MNs using hyaluronic acid methacryloyl (HAMA), hyaluronic acid (HA), and polyvinyl pyrrolidone (PVP). MNs have the advantages of small size, easy operation, good strength, rapid dissolution, and one-time delivery of two drugs. Morphological test results showed that the HAMA-HA-PVP MNs were small and intact in structure. The mechanical strength and mucosal insertion test results indicated the HAMA-HA-PVP MNs had appropriate strength and could penetrate the mucosal cuticle quickly to achieve transmucosal drug delivery. The in vitro and in vivo experiment results of the double-layer fluorescent dyes simulating drug release revealed that MNs had good solubility and achieved stratified release of the model drugs. The results of the in vivo and in vitro biosafety tests also indicated that the HAMA-HA-PVP MNs were biosafe materials. The therapeutic effect of drug-loaded HAMA-HA-PVP MNs in the rat oral mucosal ulcer model demonstrated that these novel HAMA-HA-PVP MNs quickly penetrated the mucosa, dissolved and effectively released the drug, and achieved sequential drug delivery. Compared to monolayer MNs, these HAMA-HA-PVP MNs can be used as double-layer drug reservoirs for controlled release, effectively releasing the drug in the MN stratification by dissolution in the presence of moisture. The need for secondary or multiple injections can be avoided, thus improving patient compliance. This drug delivery system can serve as an efficient, multipermeable, mucosal, and needle-free alternative for biomedical applications.
Cited By
This article is cited by 2 publications.
- Yunli Xia, Ke Xu, Maoqi Luo, Zeya Li, Siwuxie He, Tao Gong, Zhirong Zhang, Li Deng. A Bilayer Microneedle for Modulated Sequential Release of Adrenaline and Lidocaine for Prolonged Local Anesthesia. ACS Applied Bio Materials 2024, 7
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, 1229-1239. https://doi.org/10.1021/acsabm.3c01128
- Puxuan Zhao, Zhiming Li, Zhixin Ling, Yanting Zheng, Hao Chang. Efficient Loading and Sustained Delivery of Methotrexate Using a Tip-Swellable Microneedle Array Patch for Psoriasis Treatment. ACS Biomaterials Science & Engineering 2024, 10
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, 921-931. https://doi.org/10.1021/acsbiomaterials.3c01810
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