Pair your accounts.

Export articles to Mendeley

Get article recommendations from ACS based on references in your Mendeley library.

Pair your accounts.

Export articles to Mendeley

Get article recommendations from ACS based on references in your Mendeley library.

You’ve supercharged your research process with ACS and Mendeley!

STEP 1:
Click to create an ACS ID

Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

MENDELEY PAIRING EXPIRED
Your Mendeley pairing has expired. Please reconnect
ACS Publications. Most Trusted. Most Cited. Most Read
Proteomics Study of DNA–Protein Crosslinks in Methylmethanesulfonate and Fe2+-EDTA-Exposed Human Cells

OR SEARCH CITATIONS

My Activity
Publications
CONTENT TYPES

Figure 1Loading Img
Download Hi-Res ImageDownload to MS-PowerPointCite This:Chem. Res. Toxicol. 2020, 33, 11, 2739-2744
    Rapid Report

    Proteomics Study of DNA–Protein Crosslinks in Methylmethanesulfonate and Fe2+-EDTA-Exposed Human Cells
    Click to copy article linkArticle link copied!

    • Yat-Hing Ham
      Yat-Hing Ham
      Department of Chemistry and Hong Kong Branch of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
      More by Yat-Hing Ham
    • K. K. Jason Chan
      K. K. Jason Chan
      Department of Chemistry and Hong Kong Branch of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
      More by K. K. Jason Chan
    • Dominik Madej
      Dominik Madej
      Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
      More by Dominik Madej
    • Henry Lam
      Henry Lam
      Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
      More by Henry Lam
      Orcidhttp://orcid.org/0000-0001-7928-0364
    • Wan Chan*
      Wan Chan
      Department of Chemistry and Hong Kong Branch of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
      *Tel.: +852 2358-7370. Fax: +852 2358-1594. E-mail: [email protected]
      More by Wan Chan
      Orcidhttp://orcid.org/0000-0001-8479-3172
    Other Access OptionsSupporting Information (1)

    Chemical Research in Toxicology

    Cite this: Chem. Res. Toxicol. 2020, 33, 11, 2739–2744
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acs.chemrestox.0c00289
    Published October 26, 2020
    Copyright © 2020 American Chemical Society
    Request reuse permissions

    Abstract

    Click to copy section linkSection link copied!
    Abstract Image

    The formation of covalently bound DNA–protein crosslinks (DPCs) is linked to the pathophysiology of cancers and many other degenerative diseases. Knowledge of the proteins that were frequently involved in forming DPCs will improve our understanding of the etiological mechanism of diseases and facilitate the establishment of preventive measures and treatment methods. By using SDS-PAGE and nano-LC coupled Orbitrap LC-MS/MS analyses, we identified, for the first time, that the major DNA-cross-linked proteins in HeLa cells exposed to a methylating agent (methylmethanesulfonate) or hydroxyl free radicals are transcription-associated proteins. In particular, histone H2B3B and poly(rC) binding protein 2 were identified as the most frequent DPC-forming proteins.

    Copyright © 2020 American Chemical Society

    Subjects

    what are subjects

    Read this article

    To access this article, please review the available access options below.

    Get instant access

    Purchase Access

    Read this article for 48 hours. Check out below using your ACS ID or as a guest.

    Recommended

    Access through Your Institution

    You may have access to this article through your institution.

    Your institution does not have access to this content. Add or change your institution or let them know you’d like them to include access.

    Supporting Information

    Click to copy section linkSection link copied!

    The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.chemrestox.0c00289.

    • Materials and method section; list of DNA-binding proteins identified using gel-electrophoresis and LC-MS/MS analyses; position mapping of DPC formation in the major DNA-binding proteins; effect of AP site scavenger on the method used for identifying DNA-binding proteins; formation of the AP sites and dR-Cys cross-links in the genomic DNA isolated from MMS and Fe2+-EDTA-treated HeLa cells; representative LC-MS/MS chromatograms and calibration curves from analysis of dR-Cys and AP sites; typical MS/MS spectra of unique peptide used in identifying PCBP2 as one of the major DNA-binding proteins, together with that of deoxyribose-containing peptides (PDF)

    Terms & Conditions

    Electronic Supporting Information files are available without a subscription to ACS Web Editions. The American Chemical Society holds a copyright ownership interest in any copyrightable Supporting Information. Files available from the ACS website may be downloaded for personal use only. Users are not otherwise permitted to reproduce, republish, redistribute, or sell any Supporting Information from the ACS website, either in whole or in part, in either machine-readable form or any other form without permission from the American Chemical Society. For permission to reproduce, republish and redistribute this material, requesters must process their own requests via the RightsLink permission system. Information about how to use the RightsLink permission system can be found at http://pubs.acs.org/page/copyright/permissions.html.

    Cited By

    Click to copy section linkSection link copied!

    This article is cited by 5 publications.

    1. Cameron Bryan, Xiaoying Wei, Zhishuo Wang, Kun Yang. In vitro eradication of abasic site-mediated DNA–peptide/protein cross-links by Escherichia coli long-patch base excision repair. Journal of Biological Chemistry 2022, 298 (7) , 102055. https://doi.org/10.1016/j.jbc.2022.102055
    2. Xiaoying Wei, Zhishuo Wang, Caroline Hinson, Kun Yang. Human TDP1, APE1 and TREX1 repair 3′-DNA–peptide/protein cross-links arising from abasic sites in vitro. Nucleic Acids Research 2022, 50 (7) , 3638-3657. https://doi.org/10.1093/nar/gkac185
    3. Pratibha P. Ghodke, F. Peter Guengerich. DNA polymerases η and κ bypass N2-guanine-O6-alkylguanine DNA alkyltransferase cross-linked DNA-peptides. Journal of Biological Chemistry 2021, 297 (4) , 101124. https://doi.org/10.1016/j.jbc.2021.101124
    4. Xiaoying Wei, Ying Peng, Cameron Bryan, Kun Yang. Mechanisms of DNA−protein cross-link formation and repair. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 2021, 1869 (8) , 140669. https://doi.org/10.1016/j.bbapap.2021.140669
    5. Pratibha P. Ghodke, Gabriela Gonzalez-Vasquez, Hui Wang, Kevin M. Johnson, Carl A. Sedgeman, F. Peter Guengerich. Enzymatic bypass of an N6-deoxyadenosine DNA–ethylene dibromide–peptide cross-link by translesion DNA polymerases. Journal of Biological Chemistry 2021, 296 , 100444. https://doi.org/10.1016/j.jbc.2021.100444
    Download PDF
    Go to Chemical Research in Toxicology
    Get e-Alerts
    Get e-Alerts

    Chemical Research in Toxicology

    Cite this: Chem. Res. Toxicol. 2020, 33, 11, 2739–2744
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acs.chemrestox.0c00289
    Published October 26, 2020
    Copyright © 2020 American Chemical Society
    Request reuse permissions

    Article Views

    784

    Altmetric

    -

    Citations

    5
    Learn about these metrics

    Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.

    Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.

    The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated.