Proteomics Study of DNA–Protein Crosslinks in Methylmethanesulfonate and Fe2+-EDTA-Exposed Human CellsClick to copy article linkArticle link copied!
- Yat-Hing HamYat-Hing HamDepartment of Chemistry and Hong Kong Branch of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong KongMore by Yat-Hing Ham
- K. K. Jason ChanK. K. Jason ChanDepartment of Chemistry and Hong Kong Branch of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong KongMore by K. K. Jason Chan
- Dominik MadejDominik MadejDepartment of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong KongMore by Dominik Madej
- Henry LamHenry LamDepartment of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong KongMore by Henry Lam
- Wan Chan*Wan Chan*Tel.: +852 2358-7370. Fax: +852 2358-1594. E-mail: [email protected]Department of Chemistry and Hong Kong Branch of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong KongMore by Wan Chan
Abstract
![Abstract Image](/cms/10.1021/acs.chemrestox.0c00289/asset/images/medium/tx0c00289_0006.gif)
The formation of covalently bound DNA–protein crosslinks (DPCs) is linked to the pathophysiology of cancers and many other degenerative diseases. Knowledge of the proteins that were frequently involved in forming DPCs will improve our understanding of the etiological mechanism of diseases and facilitate the establishment of preventive measures and treatment methods. By using SDS-PAGE and nano-LC coupled Orbitrap LC-MS/MS analyses, we identified, for the first time, that the major DNA-cross-linked proteins in HeLa cells exposed to a methylating agent (methylmethanesulfonate) or hydroxyl free radicals are transcription-associated proteins. In particular, histone H2B3B and poly(rC) binding protein 2 were identified as the most frequent DPC-forming proteins.
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